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Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients
BACKGROUND AND PURPOSE: Gastroenteritis and the clinical profile caused by Microsporidia, an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Mazandaran University of Medical Sciences
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747587/ https://www.ncbi.nlm.nih.gov/pubmed/29302628 http://dx.doi.org/10.29252/cmm.3.1.30 |
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author | Nooshadokht, M Sharifi, I Mohammadi, MA Pirestani, M Afgar, A Mahootchi, A Salari, S |
author_facet | Nooshadokht, M Sharifi, I Mohammadi, MA Pirestani, M Afgar, A Mahootchi, A Salari, S |
author_sort | Nooshadokht, M |
collection | PubMed |
description | BACKGROUND AND PURPOSE: Gastroenteritis and the clinical profile caused by Microsporidia, an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to the limitations of the microscopy-based methods, several DNA-based methods such as polymerase chain reaction (PCR) have recently been developed to enhance diagnosis sensitivity. Therefore, we sought to evaluate the rate of infection in immunocompromised patients as compared with immunocompetent patients in Kerman, Iran. MATERIALS AND METHODS: We collected stool samples of 199 human subjects (116 males and 83 females), aged 1 to 69 years old. They were divided into immunocompromised (i.e., AIDS [n=72] and cancer-positive patients [n=59]) and immunocompetent (n=68) groups. We comparatively examined the fecal materials using the microscopy and PCR methods. RESULTS: The overall prevalence rate of Microsporidia infection was 10.05% (20/199). Entrocytozoon bieneusi was the only species within the Microsporidia genus that was identified in 14.5% (19/131) of the immunocompromised patients and 1.47% (1/68) of the immunocompetent individuals. CONCLUSION: Considering the higher prevalence rate of microsporidiosis in patients with immunodeficiency (10.03%), we suggest performing sensitive and specific techniques such as PCR for the detection of these parasites in immunocompromised patients. |
format | Online Article Text |
id | pubmed-5747587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Mazandaran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-57475872018-01-04 Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients Nooshadokht, M Sharifi, I Mohammadi, MA Pirestani, M Afgar, A Mahootchi, A Salari, S Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Gastroenteritis and the clinical profile caused by Microsporidia, an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to the limitations of the microscopy-based methods, several DNA-based methods such as polymerase chain reaction (PCR) have recently been developed to enhance diagnosis sensitivity. Therefore, we sought to evaluate the rate of infection in immunocompromised patients as compared with immunocompetent patients in Kerman, Iran. MATERIALS AND METHODS: We collected stool samples of 199 human subjects (116 males and 83 females), aged 1 to 69 years old. They were divided into immunocompromised (i.e., AIDS [n=72] and cancer-positive patients [n=59]) and immunocompetent (n=68) groups. We comparatively examined the fecal materials using the microscopy and PCR methods. RESULTS: The overall prevalence rate of Microsporidia infection was 10.05% (20/199). Entrocytozoon bieneusi was the only species within the Microsporidia genus that was identified in 14.5% (19/131) of the immunocompromised patients and 1.47% (1/68) of the immunocompetent individuals. CONCLUSION: Considering the higher prevalence rate of microsporidiosis in patients with immunodeficiency (10.03%), we suggest performing sensitive and specific techniques such as PCR for the detection of these parasites in immunocompromised patients. Mazandaran University of Medical Sciences 2017-03 /pmc/articles/PMC5747587/ /pubmed/29302628 http://dx.doi.org/10.29252/cmm.3.1.30 Text en © 2017, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nooshadokht, M Sharifi, I Mohammadi, MA Pirestani, M Afgar, A Mahootchi, A Salari, S Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients |
title | Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients |
title_full | Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients |
title_fullStr | Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients |
title_full_unstemmed | Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients |
title_short | Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients |
title_sort | intestinal microsporidiosis in iran: infection in immune-compromised and immunocompetent patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747587/ https://www.ncbi.nlm.nih.gov/pubmed/29302628 http://dx.doi.org/10.29252/cmm.3.1.30 |
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