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Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model

The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and...

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Autores principales: Lan, Chuangxin, Chen, Dong, Liang, Xiongfa, Huang, Jian, Zeng, Tao, Duan, Xiaolu, Chen, Kang, Lai, Yongchang, Yang, Dong, Li, Shujue, Jiang, Chonghe, Wu, Wenqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748115/
https://www.ncbi.nlm.nih.gov/pubmed/29392139
http://dx.doi.org/10.1155/2017/8306736
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author Lan, Chuangxin
Chen, Dong
Liang, Xiongfa
Huang, Jian
Zeng, Tao
Duan, Xiaolu
Chen, Kang
Lai, Yongchang
Yang, Dong
Li, Shujue
Jiang, Chonghe
Wu, Wenqi
author_facet Lan, Chuangxin
Chen, Dong
Liang, Xiongfa
Huang, Jian
Zeng, Tao
Duan, Xiaolu
Chen, Kang
Lai, Yongchang
Yang, Dong
Li, Shujue
Jiang, Chonghe
Wu, Wenqi
author_sort Lan, Chuangxin
collection PubMed
description The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and stone-forming group. 24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks. MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles. To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed. A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group. Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway. Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination. The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism.
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spelling pubmed-57481152018-02-01 Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model Lan, Chuangxin Chen, Dong Liang, Xiongfa Huang, Jian Zeng, Tao Duan, Xiaolu Chen, Kang Lai, Yongchang Yang, Dong Li, Shujue Jiang, Chonghe Wu, Wenqi Biomed Res Int Research Article The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and stone-forming group. 24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks. MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles. To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed. A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group. Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway. Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination. The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism. Hindawi 2017 2017-12-17 /pmc/articles/PMC5748115/ /pubmed/29392139 http://dx.doi.org/10.1155/2017/8306736 Text en Copyright © 2017 Chuangxin Lan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lan, Chuangxin
Chen, Dong
Liang, Xiongfa
Huang, Jian
Zeng, Tao
Duan, Xiaolu
Chen, Kang
Lai, Yongchang
Yang, Dong
Li, Shujue
Jiang, Chonghe
Wu, Wenqi
Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
title Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
title_full Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
title_fullStr Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
title_full_unstemmed Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
title_short Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
title_sort integrative analysis of mirna and mrna expression profiles in calcium oxalate nephrolithiasis rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748115/
https://www.ncbi.nlm.nih.gov/pubmed/29392139
http://dx.doi.org/10.1155/2017/8306736
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