Cargando…
Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model
The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748115/ https://www.ncbi.nlm.nih.gov/pubmed/29392139 http://dx.doi.org/10.1155/2017/8306736 |
_version_ | 1783289361015504896 |
---|---|
author | Lan, Chuangxin Chen, Dong Liang, Xiongfa Huang, Jian Zeng, Tao Duan, Xiaolu Chen, Kang Lai, Yongchang Yang, Dong Li, Shujue Jiang, Chonghe Wu, Wenqi |
author_facet | Lan, Chuangxin Chen, Dong Liang, Xiongfa Huang, Jian Zeng, Tao Duan, Xiaolu Chen, Kang Lai, Yongchang Yang, Dong Li, Shujue Jiang, Chonghe Wu, Wenqi |
author_sort | Lan, Chuangxin |
collection | PubMed |
description | The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and stone-forming group. 24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks. MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles. To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed. A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group. Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway. Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination. The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism. |
format | Online Article Text |
id | pubmed-5748115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-57481152018-02-01 Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model Lan, Chuangxin Chen, Dong Liang, Xiongfa Huang, Jian Zeng, Tao Duan, Xiaolu Chen, Kang Lai, Yongchang Yang, Dong Li, Shujue Jiang, Chonghe Wu, Wenqi Biomed Res Int Research Article The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and stone-forming group. 24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks. MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles. To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed. A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group. Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway. Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination. The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism. Hindawi 2017 2017-12-17 /pmc/articles/PMC5748115/ /pubmed/29392139 http://dx.doi.org/10.1155/2017/8306736 Text en Copyright © 2017 Chuangxin Lan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lan, Chuangxin Chen, Dong Liang, Xiongfa Huang, Jian Zeng, Tao Duan, Xiaolu Chen, Kang Lai, Yongchang Yang, Dong Li, Shujue Jiang, Chonghe Wu, Wenqi Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model |
title | Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model |
title_full | Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model |
title_fullStr | Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model |
title_full_unstemmed | Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model |
title_short | Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model |
title_sort | integrative analysis of mirna and mrna expression profiles in calcium oxalate nephrolithiasis rat model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748115/ https://www.ncbi.nlm.nih.gov/pubmed/29392139 http://dx.doi.org/10.1155/2017/8306736 |
work_keys_str_mv | AT lanchuangxin integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT chendong integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT liangxiongfa integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT huangjian integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT zengtao integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT duanxiaolu integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT chenkang integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT laiyongchang integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT yangdong integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT lishujue integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT jiangchonghe integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel AT wuwenqi integrativeanalysisofmirnaandmrnaexpressionprofilesincalciumoxalatenephrolithiasisratmodel |