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Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis

Secretion of extracellular vesicles (EVs) is a common feature of both eukaryotic and prokaryotic cells. Isolated EVs have been shown to contain different types of molecules, including proteins and nucleic acids, and are reported to be key players in intercellular communication. Little is known, howe...

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Autores principales: Lagos, Leidy, Tandberg, Julia, Kashulin-Bekkelund, Alexander, Colquhoun, Duncan J., Sørum, Henning, Winther-Larsen, Hanne C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748569/
https://www.ncbi.nlm.nih.gov/pubmed/29194379
http://dx.doi.org/10.3390/proteomes5040034
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author Lagos, Leidy
Tandberg, Julia
Kashulin-Bekkelund, Alexander
Colquhoun, Duncan J.
Sørum, Henning
Winther-Larsen, Hanne C.
author_facet Lagos, Leidy
Tandberg, Julia
Kashulin-Bekkelund, Alexander
Colquhoun, Duncan J.
Sørum, Henning
Winther-Larsen, Hanne C.
author_sort Lagos, Leidy
collection PubMed
description Secretion of extracellular vesicles (EVs) is a common feature of both eukaryotic and prokaryotic cells. Isolated EVs have been shown to contain different types of molecules, including proteins and nucleic acids, and are reported to be key players in intercellular communication. Little is known, however, of EV secretion in fish, or the effect of infection on EV release and content. In the present study, EVs were isolated from the serum of healthy and Piscirickettsia salmonis infected Atlantic salmon in order to evaluate the effect of infection on EV secretion. P. salmonis is facultative intracellular bacterium that causes a systemic infection disease in farmed salmonids. EVs isolated from both infected and non-infected fish had an average diameter of 230–300 nm, as confirmed by transmission electron microscopy, nanoparticle tracking, and flow cytometry. Mass spectrometry identified 180 proteins in serum EVs from both groups of fish. Interestingly, 35 unique proteins were identified in serum EVs isolated from the fish infected with P. salmonis. These unique proteins included proteasomes subunits, granulins, and major histocompatibility class I and II. Our results suggest that EV release could be part of a mechanism in which host stimulatory molecules are released from infected cells to promote an immune response.
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spelling pubmed-57485692018-01-07 Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis Lagos, Leidy Tandberg, Julia Kashulin-Bekkelund, Alexander Colquhoun, Duncan J. Sørum, Henning Winther-Larsen, Hanne C. Proteomes Article Secretion of extracellular vesicles (EVs) is a common feature of both eukaryotic and prokaryotic cells. Isolated EVs have been shown to contain different types of molecules, including proteins and nucleic acids, and are reported to be key players in intercellular communication. Little is known, however, of EV secretion in fish, or the effect of infection on EV release and content. In the present study, EVs were isolated from the serum of healthy and Piscirickettsia salmonis infected Atlantic salmon in order to evaluate the effect of infection on EV secretion. P. salmonis is facultative intracellular bacterium that causes a systemic infection disease in farmed salmonids. EVs isolated from both infected and non-infected fish had an average diameter of 230–300 nm, as confirmed by transmission electron microscopy, nanoparticle tracking, and flow cytometry. Mass spectrometry identified 180 proteins in serum EVs from both groups of fish. Interestingly, 35 unique proteins were identified in serum EVs isolated from the fish infected with P. salmonis. These unique proteins included proteasomes subunits, granulins, and major histocompatibility class I and II. Our results suggest that EV release could be part of a mechanism in which host stimulatory molecules are released from infected cells to promote an immune response. MDPI 2017-12-01 /pmc/articles/PMC5748569/ /pubmed/29194379 http://dx.doi.org/10.3390/proteomes5040034 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lagos, Leidy
Tandberg, Julia
Kashulin-Bekkelund, Alexander
Colquhoun, Duncan J.
Sørum, Henning
Winther-Larsen, Hanne C.
Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis
title Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis
title_full Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis
title_fullStr Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis
title_full_unstemmed Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis
title_short Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis
title_sort isolation and characterization of serum extracellular vesicles (evs) from atlantic salmon infected with piscirickettsia salmonis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748569/
https://www.ncbi.nlm.nih.gov/pubmed/29194379
http://dx.doi.org/10.3390/proteomes5040034
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