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Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea

Deciphering the ecological traits of abundant marine bacteria is a major challenge in marine microbial ecology. In the current study, we linked compositional and functional predictions to elucidate such traits for abundant bacterioplankton lineages in the North Sea. For this purpose, we investigated...

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Autores principales: Wemheuer, Bernd, Wemheuer, Franziska, Meier, Dimitri, Billerbeck, Sara, Giebel, Helge-Ansgar, Simon, Meinhard, Scherber, Christoph, Daniel, Rolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748577/
https://www.ncbi.nlm.nih.gov/pubmed/29113091
http://dx.doi.org/10.3390/microorganisms5040068
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author Wemheuer, Bernd
Wemheuer, Franziska
Meier, Dimitri
Billerbeck, Sara
Giebel, Helge-Ansgar
Simon, Meinhard
Scherber, Christoph
Daniel, Rolf
author_facet Wemheuer, Bernd
Wemheuer, Franziska
Meier, Dimitri
Billerbeck, Sara
Giebel, Helge-Ansgar
Simon, Meinhard
Scherber, Christoph
Daniel, Rolf
author_sort Wemheuer, Bernd
collection PubMed
description Deciphering the ecological traits of abundant marine bacteria is a major challenge in marine microbial ecology. In the current study, we linked compositional and functional predictions to elucidate such traits for abundant bacterioplankton lineages in the North Sea. For this purpose, we investigated entire and active bacterioplankton composition along a transect ranging from the German Bight to the northern North Sea by pyrotag sequencing of bacterial 16S rRNA genes and transcripts. Functional profiles were inferred from 16S rRNA data using Tax4Fun. Bacterioplankton communities were dominated by well-known marine lineages including clusters/genera that are affiliated with the Roseobacter group and the Flavobacteria. Variations in community composition and function were significantly explained by measured environmental and microbial properties. Turnover of dissolved free amino acids (DFAA) showed the strongest correlation to community composition and function. We applied multinomial models, which enabled us to identify bacterial lineages involved in DFAA turnover. For instance, the genus Planktomarina was more abundant at higher DFAA turnover rates, suggesting its vital role in amino acid degradation. Functional predictions further indicated that Planktomarina is involved in leucine and isoleucine degradation. Overall, our results provide novel insights into the biogeochemical significance of abundant bacterioplankton lineages in the North Sea.
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spelling pubmed-57485772018-01-07 Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea Wemheuer, Bernd Wemheuer, Franziska Meier, Dimitri Billerbeck, Sara Giebel, Helge-Ansgar Simon, Meinhard Scherber, Christoph Daniel, Rolf Microorganisms Article Deciphering the ecological traits of abundant marine bacteria is a major challenge in marine microbial ecology. In the current study, we linked compositional and functional predictions to elucidate such traits for abundant bacterioplankton lineages in the North Sea. For this purpose, we investigated entire and active bacterioplankton composition along a transect ranging from the German Bight to the northern North Sea by pyrotag sequencing of bacterial 16S rRNA genes and transcripts. Functional profiles were inferred from 16S rRNA data using Tax4Fun. Bacterioplankton communities were dominated by well-known marine lineages including clusters/genera that are affiliated with the Roseobacter group and the Flavobacteria. Variations in community composition and function were significantly explained by measured environmental and microbial properties. Turnover of dissolved free amino acids (DFAA) showed the strongest correlation to community composition and function. We applied multinomial models, which enabled us to identify bacterial lineages involved in DFAA turnover. For instance, the genus Planktomarina was more abundant at higher DFAA turnover rates, suggesting its vital role in amino acid degradation. Functional predictions further indicated that Planktomarina is involved in leucine and isoleucine degradation. Overall, our results provide novel insights into the biogeochemical significance of abundant bacterioplankton lineages in the North Sea. MDPI 2017-11-05 /pmc/articles/PMC5748577/ /pubmed/29113091 http://dx.doi.org/10.3390/microorganisms5040068 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wemheuer, Bernd
Wemheuer, Franziska
Meier, Dimitri
Billerbeck, Sara
Giebel, Helge-Ansgar
Simon, Meinhard
Scherber, Christoph
Daniel, Rolf
Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea
title Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea
title_full Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea
title_fullStr Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea
title_full_unstemmed Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea
title_short Linking Compositional and Functional Predictions to Decipher the Biogeochemical Significance in DFAA Turnover of Abundant Bacterioplankton Lineages in the North Sea
title_sort linking compositional and functional predictions to decipher the biogeochemical significance in dfaa turnover of abundant bacterioplankton lineages in the north sea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748577/
https://www.ncbi.nlm.nih.gov/pubmed/29113091
http://dx.doi.org/10.3390/microorganisms5040068
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