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Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms
Treating infections organized in biofilms is a challenge due to the resistance of the pathogens against antibiotics and host immune cells. Many fungi grow in a wet environment, favorable for the growth of bacterial biofilms, and we speculated that fungi possess some strategies to control these bacte...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748589/ https://www.ncbi.nlm.nih.gov/pubmed/29231891 http://dx.doi.org/10.3390/microorganisms5040080 |
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author | Yuyama, Kamila Tomoko Chepkirui, Clara Wendt, Lucile Fortkamp, Diana Stadler, Marc Abraham, Wolf-Rainer |
author_facet | Yuyama, Kamila Tomoko Chepkirui, Clara Wendt, Lucile Fortkamp, Diana Stadler, Marc Abraham, Wolf-Rainer |
author_sort | Yuyama, Kamila Tomoko |
collection | PubMed |
description | Treating infections organized in biofilms is a challenge due to the resistance of the pathogens against antibiotics and host immune cells. Many fungi grow in a wet environment, favorable for the growth of bacterial biofilms, and we speculated that fungi possess some strategies to control these bacterial biofilms. A fungus identified as Hypoxylon fragiforme, was collected in the Harz Mountains, Germany, and its mycelial culture was fermented in different culture media for 67 days to test its biological potential against bacterial biofilms. Sclerin, sclerin diacid and its 3-methyl monoester (methyl 1-(5-hydroxy-6-carboxylic-2,3,4-trimethylphenyl) propionate) are here described for the first time from this fungus. Sclerin and its diacid interfered with the biofilm formation of the pathogen Staphylococcus aureus, inhibiting 86% and 80% of the biofilm at 256 μg mL(−1), respectively, but not killing the bacterium. Interestingly, the monomethylester of sclerin diacid was inactive. Although these compounds did not possess any activity against a pre-formed biofilm, they prevented its formation at subtoxic concentrations. Furthermore, sclerin and its diacid displayed a high specificity against Staphylococcus aureus, indicating a good strategy against pathogenic biofilms when combined with antibiotics. |
format | Online Article Text |
id | pubmed-5748589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57485892018-01-07 Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms Yuyama, Kamila Tomoko Chepkirui, Clara Wendt, Lucile Fortkamp, Diana Stadler, Marc Abraham, Wolf-Rainer Microorganisms Article Treating infections organized in biofilms is a challenge due to the resistance of the pathogens against antibiotics and host immune cells. Many fungi grow in a wet environment, favorable for the growth of bacterial biofilms, and we speculated that fungi possess some strategies to control these bacterial biofilms. A fungus identified as Hypoxylon fragiforme, was collected in the Harz Mountains, Germany, and its mycelial culture was fermented in different culture media for 67 days to test its biological potential against bacterial biofilms. Sclerin, sclerin diacid and its 3-methyl monoester (methyl 1-(5-hydroxy-6-carboxylic-2,3,4-trimethylphenyl) propionate) are here described for the first time from this fungus. Sclerin and its diacid interfered with the biofilm formation of the pathogen Staphylococcus aureus, inhibiting 86% and 80% of the biofilm at 256 μg mL(−1), respectively, but not killing the bacterium. Interestingly, the monomethylester of sclerin diacid was inactive. Although these compounds did not possess any activity against a pre-formed biofilm, they prevented its formation at subtoxic concentrations. Furthermore, sclerin and its diacid displayed a high specificity against Staphylococcus aureus, indicating a good strategy against pathogenic biofilms when combined with antibiotics. MDPI 2017-12-12 /pmc/articles/PMC5748589/ /pubmed/29231891 http://dx.doi.org/10.3390/microorganisms5040080 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yuyama, Kamila Tomoko Chepkirui, Clara Wendt, Lucile Fortkamp, Diana Stadler, Marc Abraham, Wolf-Rainer Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms |
title | Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms |
title_full | Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms |
title_fullStr | Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms |
title_full_unstemmed | Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms |
title_short | Bioactive Compounds Produced by Hypoxylon fragiforme against Staphylococcus aureus Biofilms |
title_sort | bioactive compounds produced by hypoxylon fragiforme against staphylococcus aureus biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748589/ https://www.ncbi.nlm.nih.gov/pubmed/29231891 http://dx.doi.org/10.3390/microorganisms5040080 |
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