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Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana

Salix matsudana is a large and rapidly-growing tree, with erect or spreading branchlets (upright willow). However, S. matsudana var. pseudomatsudana is one of the varietas, with pendulous branchlets (weeping willow). It has high ornamental value for its graceful pendulous branches. In order to study...

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Autores principales: Liu, Juanjuan, Zeng, Yanfei, Yan, Pengcheng, He, Caiyun, Zhang, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748677/
https://www.ncbi.nlm.nih.gov/pubmed/29189719
http://dx.doi.org/10.3390/genes8120359
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author Liu, Juanjuan
Zeng, Yanfei
Yan, Pengcheng
He, Caiyun
Zhang, Jianguo
author_facet Liu, Juanjuan
Zeng, Yanfei
Yan, Pengcheng
He, Caiyun
Zhang, Jianguo
author_sort Liu, Juanjuan
collection PubMed
description Salix matsudana is a large and rapidly-growing tree, with erect or spreading branchlets (upright willow). However, S. matsudana var. pseudomatsudana is one of the varietas, with pendulous branchlets (weeping willow). It has high ornamental value for its graceful pendulous branches. In order to study the molecular basis for this weeping trait, leaves and stems collected at different developmental stages were analyzed using RNA-seq coupled with digital gene expression. Although weeping trees are used worldwide as landscape plants, little is known about the genes that control weeping. Our growth results indicated that branches in weeping willow developed and elongated throughout all developmental stages, but branches in upright willow grew rapidly in the initial stages and then grew slowly and began shoot branching in the middle stages. A total of 613 hormone-related genes were differentially expressed in willow development. Among these, genes associated with auxin and gibberellin (GA) were highly likely to be responsible for the weeping trait, and genes associated with auxin and ethylene probably play crucial roles in shoot elongation. The genes with differential expression patterns were used to construct a network that regulated stem development, and auxin-related genes were identified as hub genes in the network in the weeping willow. Our results suggest an important role of gibberellin and auxin in regulating the weeping trait in Salix matsudana. This is the first report on the molecular aspects of hormonal effects on weeping trait in willow using transcriptomics and helps in dissecting the molecular mechanisms by which the weeping trait is controlled.
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spelling pubmed-57486772018-01-07 Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana Liu, Juanjuan Zeng, Yanfei Yan, Pengcheng He, Caiyun Zhang, Jianguo Genes (Basel) Article Salix matsudana is a large and rapidly-growing tree, with erect or spreading branchlets (upright willow). However, S. matsudana var. pseudomatsudana is one of the varietas, with pendulous branchlets (weeping willow). It has high ornamental value for its graceful pendulous branches. In order to study the molecular basis for this weeping trait, leaves and stems collected at different developmental stages were analyzed using RNA-seq coupled with digital gene expression. Although weeping trees are used worldwide as landscape plants, little is known about the genes that control weeping. Our growth results indicated that branches in weeping willow developed and elongated throughout all developmental stages, but branches in upright willow grew rapidly in the initial stages and then grew slowly and began shoot branching in the middle stages. A total of 613 hormone-related genes were differentially expressed in willow development. Among these, genes associated with auxin and gibberellin (GA) were highly likely to be responsible for the weeping trait, and genes associated with auxin and ethylene probably play crucial roles in shoot elongation. The genes with differential expression patterns were used to construct a network that regulated stem development, and auxin-related genes were identified as hub genes in the network in the weeping willow. Our results suggest an important role of gibberellin and auxin in regulating the weeping trait in Salix matsudana. This is the first report on the molecular aspects of hormonal effects on weeping trait in willow using transcriptomics and helps in dissecting the molecular mechanisms by which the weeping trait is controlled. MDPI 2017-11-30 /pmc/articles/PMC5748677/ /pubmed/29189719 http://dx.doi.org/10.3390/genes8120359 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Juanjuan
Zeng, Yanfei
Yan, Pengcheng
He, Caiyun
Zhang, Jianguo
Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana
title Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana
title_full Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana
title_fullStr Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana
title_full_unstemmed Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana
title_short Transcriptional and Hormonal Regulation of Weeping Trait in Salix matsudana
title_sort transcriptional and hormonal regulation of weeping trait in salix matsudana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748677/
https://www.ncbi.nlm.nih.gov/pubmed/29189719
http://dx.doi.org/10.3390/genes8120359
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