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Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress

Small RNAs play an important role in regulating plant responses to abiotic stress. Depending on the method of salt application, whether sudden or gradual, plants may experience either salt shock or salt stress, respectively. In this study, small RNA expression in response to salt shock and long-term...

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Autores principales: Yin, Zujun, Han, Xiulan, Li, Yan, Wang, Junjuan, Wang, Delong, Wang, Shuai, Fu, Xiaoqiong, Ye, Wuwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748687/
https://www.ncbi.nlm.nih.gov/pubmed/29206160
http://dx.doi.org/10.3390/genes8120369
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author Yin, Zujun
Han, Xiulan
Li, Yan
Wang, Junjuan
Wang, Delong
Wang, Shuai
Fu, Xiaoqiong
Ye, Wuwei
author_facet Yin, Zujun
Han, Xiulan
Li, Yan
Wang, Junjuan
Wang, Delong
Wang, Shuai
Fu, Xiaoqiong
Ye, Wuwei
author_sort Yin, Zujun
collection PubMed
description Small RNAs play an important role in regulating plant responses to abiotic stress. Depending on the method of salt application, whether sudden or gradual, plants may experience either salt shock or salt stress, respectively. In this study, small RNA expression in response to salt shock and long-term salt stress in parallel experiments was described. Cotton small RNA libraries were constructed and sequenced under normal conditions, as well as sudden and gradual salt application. A total of 225 cotton microRNAs (miRNAs) were identified and of these 24 were novel miRNAs. There were 88 and 75 miRNAs with differential expression under the salt shock and long-term salt stress, respectively. Thirty one transcripts were found to be targets of 20 miRNA families. Eight targets showed a negative correlation in expression with their corresponding miRNAs. We also identified two TAS3s with two near-identical 21-nt trans-acting small interfering RNA (tasiRNA)-Auxin Response Factors (ARFs) that coaligned with the phases D7(+) and D8(+) in three Gossypium species. The miR390/tasiRNA-ARFs/ARF4 pathway was identified and showed altered expression under salt stress. The identification of these small RNAs as well as elucidating their functional significance broadens our understanding of post-transcriptional gene regulation in response to salt stress.
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spelling pubmed-57486872018-01-07 Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress Yin, Zujun Han, Xiulan Li, Yan Wang, Junjuan Wang, Delong Wang, Shuai Fu, Xiaoqiong Ye, Wuwei Genes (Basel) Article Small RNAs play an important role in regulating plant responses to abiotic stress. Depending on the method of salt application, whether sudden or gradual, plants may experience either salt shock or salt stress, respectively. In this study, small RNA expression in response to salt shock and long-term salt stress in parallel experiments was described. Cotton small RNA libraries were constructed and sequenced under normal conditions, as well as sudden and gradual salt application. A total of 225 cotton microRNAs (miRNAs) were identified and of these 24 were novel miRNAs. There were 88 and 75 miRNAs with differential expression under the salt shock and long-term salt stress, respectively. Thirty one transcripts were found to be targets of 20 miRNA families. Eight targets showed a negative correlation in expression with their corresponding miRNAs. We also identified two TAS3s with two near-identical 21-nt trans-acting small interfering RNA (tasiRNA)-Auxin Response Factors (ARFs) that coaligned with the phases D7(+) and D8(+) in three Gossypium species. The miR390/tasiRNA-ARFs/ARF4 pathway was identified and showed altered expression under salt stress. The identification of these small RNAs as well as elucidating their functional significance broadens our understanding of post-transcriptional gene regulation in response to salt stress. MDPI 2017-12-05 /pmc/articles/PMC5748687/ /pubmed/29206160 http://dx.doi.org/10.3390/genes8120369 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yin, Zujun
Han, Xiulan
Li, Yan
Wang, Junjuan
Wang, Delong
Wang, Shuai
Fu, Xiaoqiong
Ye, Wuwei
Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress
title Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress
title_full Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress
title_fullStr Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress
title_full_unstemmed Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress
title_short Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress
title_sort comparative analysis of cotton small rnas and their target genes in response to salt stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748687/
https://www.ncbi.nlm.nih.gov/pubmed/29206160
http://dx.doi.org/10.3390/genes8120369
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