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Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata

The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial st...

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Autores principales: Nguyen, Hien P., Miwa, Hiroki, Kaneko, Takakazu, Sato, Shusei, Okazaki, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748692/
https://www.ncbi.nlm.nih.gov/pubmed/29292795
http://dx.doi.org/10.3390/genes8120374
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author Nguyen, Hien P.
Miwa, Hiroki
Kaneko, Takakazu
Sato, Shusei
Okazaki, Shin
author_facet Nguyen, Hien P.
Miwa, Hiroki
Kaneko, Takakazu
Sato, Shusei
Okazaki, Shin
author_sort Nguyen, Hien P.
collection PubMed
description The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1) and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS), which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1.
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spelling pubmed-57486922018-01-07 Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata Nguyen, Hien P. Miwa, Hiroki Kaneko, Takakazu Sato, Shusei Okazaki, Shin Genes (Basel) Article The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1) and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS), which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1. MDPI 2017-12-08 /pmc/articles/PMC5748692/ /pubmed/29292795 http://dx.doi.org/10.3390/genes8120374 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nguyen, Hien P.
Miwa, Hiroki
Kaneko, Takakazu
Sato, Shusei
Okazaki, Shin
Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
title Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
title_full Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
title_fullStr Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
title_full_unstemmed Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
title_short Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
title_sort identification of bradyrhizobium elkanii genes involved in incompatibility with vigna radiata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748692/
https://www.ncbi.nlm.nih.gov/pubmed/29292795
http://dx.doi.org/10.3390/genes8120374
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