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Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial st...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748692/ https://www.ncbi.nlm.nih.gov/pubmed/29292795 http://dx.doi.org/10.3390/genes8120374 |
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author | Nguyen, Hien P. Miwa, Hiroki Kaneko, Takakazu Sato, Shusei Okazaki, Shin |
author_facet | Nguyen, Hien P. Miwa, Hiroki Kaneko, Takakazu Sato, Shusei Okazaki, Shin |
author_sort | Nguyen, Hien P. |
collection | PubMed |
description | The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1) and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS), which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1. |
format | Online Article Text |
id | pubmed-5748692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57486922018-01-07 Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata Nguyen, Hien P. Miwa, Hiroki Kaneko, Takakazu Sato, Shusei Okazaki, Shin Genes (Basel) Article The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1) and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS), which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1. MDPI 2017-12-08 /pmc/articles/PMC5748692/ /pubmed/29292795 http://dx.doi.org/10.3390/genes8120374 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nguyen, Hien P. Miwa, Hiroki Kaneko, Takakazu Sato, Shusei Okazaki, Shin Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata |
title | Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata |
title_full | Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata |
title_fullStr | Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata |
title_full_unstemmed | Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata |
title_short | Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata |
title_sort | identification of bradyrhizobium elkanii genes involved in incompatibility with vigna radiata |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748692/ https://www.ncbi.nlm.nih.gov/pubmed/29292795 http://dx.doi.org/10.3390/genes8120374 |
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