Cargando…

Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs

Sialic acid (SA) is a key component of gangliosides and neural cell adhesion molecules important during neurodevelopment. Human milk contains SA in the form of sialyllactose (SL) an abundant oligosaccharide. To better understand the potential role of dietary SL on neurodevelopment, the effects of va...

Descripción completa

Detalles Bibliográficos
Autores principales: Mudd, Austin T., Fleming, Stephen A., Labhart, Beau, Chichlowski, Maciej, Berg, Brian M., Donovan, Sharon M., Dilger, Ryan N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748748/
https://www.ncbi.nlm.nih.gov/pubmed/29182578
http://dx.doi.org/10.3390/nu9121297
_version_ 1783289463832576000
author Mudd, Austin T.
Fleming, Stephen A.
Labhart, Beau
Chichlowski, Maciej
Berg, Brian M.
Donovan, Sharon M.
Dilger, Ryan N.
author_facet Mudd, Austin T.
Fleming, Stephen A.
Labhart, Beau
Chichlowski, Maciej
Berg, Brian M.
Donovan, Sharon M.
Dilger, Ryan N.
author_sort Mudd, Austin T.
collection PubMed
description Sialic acid (SA) is a key component of gangliosides and neural cell adhesion molecules important during neurodevelopment. Human milk contains SA in the form of sialyllactose (SL) an abundant oligosaccharide. To better understand the potential role of dietary SL on neurodevelopment, the effects of varying doses of dietary SL on brain SA content and neuroimaging markers of development were assessed in a newborn piglet model. Thirty-eight male pigs were provided one of four experimental diets from 2 to 32 days of age. Diets were formulated to contain: 0 mg SL/L (CON), 130 mg SL/L (LOW), 380 mg SL/L (MOD) or 760 mg SL/L (HIGH). At 32 or 33 days of age, all pigs were subjected to magnetic resonance imaging (MRI) to assess brain development. After MRI, pig serum and brains were collected and total, free and bound SA was analyzed. Results from this study indicate dietary SL influenced (p = 0.05) bound SA in the prefrontal cortex and the ratio of free SA to bound SA in the hippocampus (p = 0.04). Diffusion tensor imaging indicated treatment effects in mean (p < 0.01), axial (p < 0.01) and radial (p = 0.01) diffusivity in the corpus callosum. Tract-based spatial statistics (TBSS) indicated differences (p < 0.05) in white matter tracts and voxel-based morphometry (VBM) indicated differences (p < 0.05) in grey matter between LOW and MOD pigs. CONT and HIGH pigs were not included in the TBSS and VBM assessments. These findings suggest the corpus callosum, prefrontal cortex and hippocampus may be differentially sensitive to dietary SL supplementation.
format Online
Article
Text
id pubmed-5748748
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57487482018-01-07 Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs Mudd, Austin T. Fleming, Stephen A. Labhart, Beau Chichlowski, Maciej Berg, Brian M. Donovan, Sharon M. Dilger, Ryan N. Nutrients Article Sialic acid (SA) is a key component of gangliosides and neural cell adhesion molecules important during neurodevelopment. Human milk contains SA in the form of sialyllactose (SL) an abundant oligosaccharide. To better understand the potential role of dietary SL on neurodevelopment, the effects of varying doses of dietary SL on brain SA content and neuroimaging markers of development were assessed in a newborn piglet model. Thirty-eight male pigs were provided one of four experimental diets from 2 to 32 days of age. Diets were formulated to contain: 0 mg SL/L (CON), 130 mg SL/L (LOW), 380 mg SL/L (MOD) or 760 mg SL/L (HIGH). At 32 or 33 days of age, all pigs were subjected to magnetic resonance imaging (MRI) to assess brain development. After MRI, pig serum and brains were collected and total, free and bound SA was analyzed. Results from this study indicate dietary SL influenced (p = 0.05) bound SA in the prefrontal cortex and the ratio of free SA to bound SA in the hippocampus (p = 0.04). Diffusion tensor imaging indicated treatment effects in mean (p < 0.01), axial (p < 0.01) and radial (p = 0.01) diffusivity in the corpus callosum. Tract-based spatial statistics (TBSS) indicated differences (p < 0.05) in white matter tracts and voxel-based morphometry (VBM) indicated differences (p < 0.05) in grey matter between LOW and MOD pigs. CONT and HIGH pigs were not included in the TBSS and VBM assessments. These findings suggest the corpus callosum, prefrontal cortex and hippocampus may be differentially sensitive to dietary SL supplementation. MDPI 2017-11-28 /pmc/articles/PMC5748748/ /pubmed/29182578 http://dx.doi.org/10.3390/nu9121297 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mudd, Austin T.
Fleming, Stephen A.
Labhart, Beau
Chichlowski, Maciej
Berg, Brian M.
Donovan, Sharon M.
Dilger, Ryan N.
Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
title Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
title_full Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
title_fullStr Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
title_full_unstemmed Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
title_short Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
title_sort dietary sialyllactose influences sialic acid concentrations in the prefrontal cortex and magnetic resonance imaging measures in corpus callosum of young pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748748/
https://www.ncbi.nlm.nih.gov/pubmed/29182578
http://dx.doi.org/10.3390/nu9121297
work_keys_str_mv AT muddaustint dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs
AT flemingstephena dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs
AT labhartbeau dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs
AT chichlowskimaciej dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs
AT bergbrianm dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs
AT donovansharonm dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs
AT dilgerryann dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs