Cargando…
Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs
Sialic acid (SA) is a key component of gangliosides and neural cell adhesion molecules important during neurodevelopment. Human milk contains SA in the form of sialyllactose (SL) an abundant oligosaccharide. To better understand the potential role of dietary SL on neurodevelopment, the effects of va...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748748/ https://www.ncbi.nlm.nih.gov/pubmed/29182578 http://dx.doi.org/10.3390/nu9121297 |
_version_ | 1783289463832576000 |
---|---|
author | Mudd, Austin T. Fleming, Stephen A. Labhart, Beau Chichlowski, Maciej Berg, Brian M. Donovan, Sharon M. Dilger, Ryan N. |
author_facet | Mudd, Austin T. Fleming, Stephen A. Labhart, Beau Chichlowski, Maciej Berg, Brian M. Donovan, Sharon M. Dilger, Ryan N. |
author_sort | Mudd, Austin T. |
collection | PubMed |
description | Sialic acid (SA) is a key component of gangliosides and neural cell adhesion molecules important during neurodevelopment. Human milk contains SA in the form of sialyllactose (SL) an abundant oligosaccharide. To better understand the potential role of dietary SL on neurodevelopment, the effects of varying doses of dietary SL on brain SA content and neuroimaging markers of development were assessed in a newborn piglet model. Thirty-eight male pigs were provided one of four experimental diets from 2 to 32 days of age. Diets were formulated to contain: 0 mg SL/L (CON), 130 mg SL/L (LOW), 380 mg SL/L (MOD) or 760 mg SL/L (HIGH). At 32 or 33 days of age, all pigs were subjected to magnetic resonance imaging (MRI) to assess brain development. After MRI, pig serum and brains were collected and total, free and bound SA was analyzed. Results from this study indicate dietary SL influenced (p = 0.05) bound SA in the prefrontal cortex and the ratio of free SA to bound SA in the hippocampus (p = 0.04). Diffusion tensor imaging indicated treatment effects in mean (p < 0.01), axial (p < 0.01) and radial (p = 0.01) diffusivity in the corpus callosum. Tract-based spatial statistics (TBSS) indicated differences (p < 0.05) in white matter tracts and voxel-based morphometry (VBM) indicated differences (p < 0.05) in grey matter between LOW and MOD pigs. CONT and HIGH pigs were not included in the TBSS and VBM assessments. These findings suggest the corpus callosum, prefrontal cortex and hippocampus may be differentially sensitive to dietary SL supplementation. |
format | Online Article Text |
id | pubmed-5748748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57487482018-01-07 Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs Mudd, Austin T. Fleming, Stephen A. Labhart, Beau Chichlowski, Maciej Berg, Brian M. Donovan, Sharon M. Dilger, Ryan N. Nutrients Article Sialic acid (SA) is a key component of gangliosides and neural cell adhesion molecules important during neurodevelopment. Human milk contains SA in the form of sialyllactose (SL) an abundant oligosaccharide. To better understand the potential role of dietary SL on neurodevelopment, the effects of varying doses of dietary SL on brain SA content and neuroimaging markers of development were assessed in a newborn piglet model. Thirty-eight male pigs were provided one of four experimental diets from 2 to 32 days of age. Diets were formulated to contain: 0 mg SL/L (CON), 130 mg SL/L (LOW), 380 mg SL/L (MOD) or 760 mg SL/L (HIGH). At 32 or 33 days of age, all pigs were subjected to magnetic resonance imaging (MRI) to assess brain development. After MRI, pig serum and brains were collected and total, free and bound SA was analyzed. Results from this study indicate dietary SL influenced (p = 0.05) bound SA in the prefrontal cortex and the ratio of free SA to bound SA in the hippocampus (p = 0.04). Diffusion tensor imaging indicated treatment effects in mean (p < 0.01), axial (p < 0.01) and radial (p = 0.01) diffusivity in the corpus callosum. Tract-based spatial statistics (TBSS) indicated differences (p < 0.05) in white matter tracts and voxel-based morphometry (VBM) indicated differences (p < 0.05) in grey matter between LOW and MOD pigs. CONT and HIGH pigs were not included in the TBSS and VBM assessments. These findings suggest the corpus callosum, prefrontal cortex and hippocampus may be differentially sensitive to dietary SL supplementation. MDPI 2017-11-28 /pmc/articles/PMC5748748/ /pubmed/29182578 http://dx.doi.org/10.3390/nu9121297 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mudd, Austin T. Fleming, Stephen A. Labhart, Beau Chichlowski, Maciej Berg, Brian M. Donovan, Sharon M. Dilger, Ryan N. Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs |
title | Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs |
title_full | Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs |
title_fullStr | Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs |
title_full_unstemmed | Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs |
title_short | Dietary Sialyllactose Influences Sialic Acid Concentrations in the Prefrontal Cortex and Magnetic Resonance Imaging Measures in Corpus Callosum of Young Pigs |
title_sort | dietary sialyllactose influences sialic acid concentrations in the prefrontal cortex and magnetic resonance imaging measures in corpus callosum of young pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748748/ https://www.ncbi.nlm.nih.gov/pubmed/29182578 http://dx.doi.org/10.3390/nu9121297 |
work_keys_str_mv | AT muddaustint dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs AT flemingstephena dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs AT labhartbeau dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs AT chichlowskimaciej dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs AT bergbrianm dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs AT donovansharonm dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs AT dilgerryann dietarysialyllactoseinfluencessialicacidconcentrationsintheprefrontalcortexandmagneticresonanceimagingmeasuresincorpuscallosumofyoungpigs |