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Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice

Increased life expectancy has promoted research on healthy aging. Aging is accompanied by increased non-specific immune activation (inflammaging) which favors the appearance of several disorders. Here, we study whether dietary supplementation with spray-dried animal plasma (SDP), which has been show...

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Autores principales: Miró, Lluïsa, Garcia-Just, Alba, Amat, Concepció, Polo, Javier, Moretó, Miquel, Pérez-Bosque, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748796/
https://www.ncbi.nlm.nih.gov/pubmed/29232896
http://dx.doi.org/10.3390/nu9121346
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author Miró, Lluïsa
Garcia-Just, Alba
Amat, Concepció
Polo, Javier
Moretó, Miquel
Pérez-Bosque, Anna
author_facet Miró, Lluïsa
Garcia-Just, Alba
Amat, Concepció
Polo, Javier
Moretó, Miquel
Pérez-Bosque, Anna
author_sort Miró, Lluïsa
collection PubMed
description Increased life expectancy has promoted research on healthy aging. Aging is accompanied by increased non-specific immune activation (inflammaging) which favors the appearance of several disorders. Here, we study whether dietary supplementation with spray-dried animal plasma (SDP), which has been shown to reduce the activation of gut-associated lymphoid tissue (GALT) in rodents challenged by S. aureus enterotoxin B (SEB), and can also prevent the effects of aging on immune system homeostasis. We first characterized GALT in a mouse model of accelerated senescence (SAMP8) at different ages (compared to mice resistant to accelerated senescence; SAMR1). Second, we analyzed the SDP effects on GALT response to an SEB challenge in SAMP8 mice. In GALT characterization, aging increased the cell number and the percentage of activated Th lymphocytes in mesenteric lymph nodes and Peyer’s patches (all, p < 0.05), as well as the expression of IL-6 and TNF-α in intestinal mucosa (both, p < 0.05). With respect to GALT response to the SEB challenge, young mice showed increased expression of intestinal IL-6 and TNF-α, as well as lymphocyte recruitment and activation (all, p < 0.05). However, the immune response of senescent mice to the SEB challenge was weak, since SEB did not change cell recruitment or the percentage of activated Th lymphocytes. Mice supplemented with SDP showed improved capacity to respond to the SEB challenge, similar to the response of the young mice. These results indicate that senescent mice have an impaired mucosal immune response characterized by unspecific GALT activation and a weak specific immune response. SDP supplementation reduces non-specific basal immune activation, allowing for the generation of specific responses.
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spelling pubmed-57487962018-01-07 Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice Miró, Lluïsa Garcia-Just, Alba Amat, Concepció Polo, Javier Moretó, Miquel Pérez-Bosque, Anna Nutrients Article Increased life expectancy has promoted research on healthy aging. Aging is accompanied by increased non-specific immune activation (inflammaging) which favors the appearance of several disorders. Here, we study whether dietary supplementation with spray-dried animal plasma (SDP), which has been shown to reduce the activation of gut-associated lymphoid tissue (GALT) in rodents challenged by S. aureus enterotoxin B (SEB), and can also prevent the effects of aging on immune system homeostasis. We first characterized GALT in a mouse model of accelerated senescence (SAMP8) at different ages (compared to mice resistant to accelerated senescence; SAMR1). Second, we analyzed the SDP effects on GALT response to an SEB challenge in SAMP8 mice. In GALT characterization, aging increased the cell number and the percentage of activated Th lymphocytes in mesenteric lymph nodes and Peyer’s patches (all, p < 0.05), as well as the expression of IL-6 and TNF-α in intestinal mucosa (both, p < 0.05). With respect to GALT response to the SEB challenge, young mice showed increased expression of intestinal IL-6 and TNF-α, as well as lymphocyte recruitment and activation (all, p < 0.05). However, the immune response of senescent mice to the SEB challenge was weak, since SEB did not change cell recruitment or the percentage of activated Th lymphocytes. Mice supplemented with SDP showed improved capacity to respond to the SEB challenge, similar to the response of the young mice. These results indicate that senescent mice have an impaired mucosal immune response characterized by unspecific GALT activation and a weak specific immune response. SDP supplementation reduces non-specific basal immune activation, allowing for the generation of specific responses. MDPI 2017-12-11 /pmc/articles/PMC5748796/ /pubmed/29232896 http://dx.doi.org/10.3390/nu9121346 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miró, Lluïsa
Garcia-Just, Alba
Amat, Concepció
Polo, Javier
Moretó, Miquel
Pérez-Bosque, Anna
Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
title Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
title_full Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
title_fullStr Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
title_full_unstemmed Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
title_short Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice
title_sort dietary animal plasma proteins improve the intestinal immune response in senescent mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748796/
https://www.ncbi.nlm.nih.gov/pubmed/29232896
http://dx.doi.org/10.3390/nu9121346
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