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The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway
Rab GTPases serve as molecular switches to regulate eukaryotic membrane trafficking pathways. The transport protein particle (TRAPP) complexes activate Rab GTPases by catalyzing GDP/GTP nucleotide exchange. In mammalian cells, there are two distinct TRAPP complexes, yet in budding yeast, four distin...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748984/ https://www.ncbi.nlm.nih.gov/pubmed/29109089 http://dx.doi.org/10.1083/jcb.201705214 |
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author | Thomas, Laura L. Joiner, Aaron M.N. Fromme, J. Christopher |
author_facet | Thomas, Laura L. Joiner, Aaron M.N. Fromme, J. Christopher |
author_sort | Thomas, Laura L. |
collection | PubMed |
description | Rab GTPases serve as molecular switches to regulate eukaryotic membrane trafficking pathways. The transport protein particle (TRAPP) complexes activate Rab GTPases by catalyzing GDP/GTP nucleotide exchange. In mammalian cells, there are two distinct TRAPP complexes, yet in budding yeast, four distinct TRAPP complexes have been reported. The apparent differences between the compositions of yeast and mammalian TRAPP complexes have prevented a clear understanding of the specific functions of TRAPP complexes in all cell types. In this study, we demonstrate that akin to mammalian cells, wild-type yeast possess only two TRAPP complexes, TRAPPII and TRAPPIII. We find that TRAPPIII plays a major role in regulating Rab activation and trafficking at the Golgi in addition to its established role in autophagy. These disparate pathways share a common regulatory GTPase Ypt1 (Rab1) that is activated by TRAPPIII. Our findings lead to a simple yet comprehensive model for TRAPPIII function in both normal and starved eukaryotic cells. |
format | Online Article Text |
id | pubmed-5748984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57489842018-07-02 The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway Thomas, Laura L. Joiner, Aaron M.N. Fromme, J. Christopher J Cell Biol Research Articles Rab GTPases serve as molecular switches to regulate eukaryotic membrane trafficking pathways. The transport protein particle (TRAPP) complexes activate Rab GTPases by catalyzing GDP/GTP nucleotide exchange. In mammalian cells, there are two distinct TRAPP complexes, yet in budding yeast, four distinct TRAPP complexes have been reported. The apparent differences between the compositions of yeast and mammalian TRAPP complexes have prevented a clear understanding of the specific functions of TRAPP complexes in all cell types. In this study, we demonstrate that akin to mammalian cells, wild-type yeast possess only two TRAPP complexes, TRAPPII and TRAPPIII. We find that TRAPPIII plays a major role in regulating Rab activation and trafficking at the Golgi in addition to its established role in autophagy. These disparate pathways share a common regulatory GTPase Ypt1 (Rab1) that is activated by TRAPPIII. Our findings lead to a simple yet comprehensive model for TRAPPIII function in both normal and starved eukaryotic cells. The Rockefeller University Press 2018-01-02 /pmc/articles/PMC5748984/ /pubmed/29109089 http://dx.doi.org/10.1083/jcb.201705214 Text en © 2018 Thomas et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Thomas, Laura L. Joiner, Aaron M.N. Fromme, J. Christopher The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway |
title | The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway |
title_full | The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway |
title_fullStr | The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway |
title_full_unstemmed | The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway |
title_short | The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway |
title_sort | trappiii complex activates the gtpase ypt1 (rab1) in the secretory pathway |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748984/ https://www.ncbi.nlm.nih.gov/pubmed/29109089 http://dx.doi.org/10.1083/jcb.201705214 |
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