Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells

Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the “Bax-negative” MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to select...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Honghong, Tassone, Cecilie, Lin, Nora, Mañas, Adriana, Zhao, Yu, Xiang, Jialing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749267/
https://www.ncbi.nlm.nih.gov/pubmed/29302381
http://dx.doi.org/10.4172/2157-7013.S8-002
_version_ 1783289554735726592
author Zhang, Honghong
Tassone, Cecilie
Lin, Nora
Mañas, Adriana
Zhao, Yu
Xiang, Jialing
author_facet Zhang, Honghong
Tassone, Cecilie
Lin, Nora
Mañas, Adriana
Zhao, Yu
Xiang, Jialing
author_sort Zhang, Honghong
collection PubMed
description Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the “Bax-negative” MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to selective chemotherapeutics. Here we show the detection of Bax microsatellite mutations and expression of BaxΔ2 proteins in human buccal cells. Our study provides a sensitive and non-invasive approach and a potential clinical application in diagnosis and treatment of MSI colon cancer patients.
format Online
Article
Text
id pubmed-5749267
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-57492672018-01-02 Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells Zhang, Honghong Tassone, Cecilie Lin, Nora Mañas, Adriana Zhao, Yu Xiang, Jialing J Cell Sci Ther Article Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the “Bax-negative” MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to selective chemotherapeutics. Here we show the detection of Bax microsatellite mutations and expression of BaxΔ2 proteins in human buccal cells. Our study provides a sensitive and non-invasive approach and a potential clinical application in diagnosis and treatment of MSI colon cancer patients. 2015-07-17 2015-09 /pmc/articles/PMC5749267/ /pubmed/29302381 http://dx.doi.org/10.4172/2157-7013.S8-002 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Zhang, Honghong
Tassone, Cecilie
Lin, Nora
Mañas, Adriana
Zhao, Yu
Xiang, Jialing
Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
title Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
title_full Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
title_fullStr Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
title_full_unstemmed Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
title_short Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells
title_sort detection of bax microsatellite mutations and baxδ2 isoform in human buccal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749267/
https://www.ncbi.nlm.nih.gov/pubmed/29302381
http://dx.doi.org/10.4172/2157-7013.S8-002
work_keys_str_mv AT zhanghonghong detectionofbaxmicrosatellitemutationsandbaxd2isoforminhumanbuccalcells
AT tassonececilie detectionofbaxmicrosatellitemutationsandbaxd2isoforminhumanbuccalcells
AT linnora detectionofbaxmicrosatellitemutationsandbaxd2isoforminhumanbuccalcells
AT manasadriana detectionofbaxmicrosatellitemutationsandbaxd2isoforminhumanbuccalcells
AT zhaoyu detectionofbaxmicrosatellitemutationsandbaxd2isoforminhumanbuccalcells
AT xiangjialing detectionofbaxmicrosatellitemutationsandbaxd2isoforminhumanbuccalcells

Ejemplares similares