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Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells
OBJECTIVE(S): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some plurip...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Mashhad University of Medical Sciences
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749353/ https://www.ncbi.nlm.nih.gov/pubmed/29299196 http://dx.doi.org/10.22038/IJBMS.2017.9464 |
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author | Salehi, Paria Motamen Foroutan, Tahereh Javeri, Arash Taha, Masoumeh Fakhr |
author_facet | Salehi, Paria Motamen Foroutan, Tahereh Javeri, Arash Taha, Masoumeh Fakhr |
author_sort | Salehi, Paria Motamen |
collection | PubMed |
description | OBJECTIVE(S): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). MATERIALS AND METHODS: Human ADSCs were isolated from subcutaneous abdominal adipose tissue and characterized by flow cytometric analysis for the expression of some mesenchymal stem cell markers and adipogenic and osteogenic differentiation. Frequent freeze-thaw technique was used to prepare cytoplasmic extract of ESCs. Plasma membranes of the ADSCs were reversibly permeabilized by streptolysin-O (SLO). Then the permeabilized ADSCs were incubated with the ESC extract and cultured in resealing medium. After reprogramming, the expression of some pluripotency genes was evaluated by RT-PCR and quantitative real-time PCR (qPCR) analyses. RESULTS: Third-passaged ADSCs showed a fibroblast-like morphology and expressed mesenchymal stem cell markers. They also showed adipogenic and osteogenic differentiation potential. QPCR analysis revealed a significant upregulation in the expression of some pluripotency genes including OCT4, SOX2, NANOG, REX1 and ESG1 in the reprogrammed ADSCs compared to the control group. CONCLUSION: These findings showed that mouse ESC extract can be used to induce reprogramming of human ADSCs. In fact, this method is applicable for reprogramming of human adult stem cells to a more pluripotent sate and may have a potential in regenerative medicine. |
format | Online Article Text |
id | pubmed-5749353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-57493532018-01-03 Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells Salehi, Paria Motamen Foroutan, Tahereh Javeri, Arash Taha, Masoumeh Fakhr Iran J Basic Med Sci Original Article OBJECTIVE(S): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). MATERIALS AND METHODS: Human ADSCs were isolated from subcutaneous abdominal adipose tissue and characterized by flow cytometric analysis for the expression of some mesenchymal stem cell markers and adipogenic and osteogenic differentiation. Frequent freeze-thaw technique was used to prepare cytoplasmic extract of ESCs. Plasma membranes of the ADSCs were reversibly permeabilized by streptolysin-O (SLO). Then the permeabilized ADSCs were incubated with the ESC extract and cultured in resealing medium. After reprogramming, the expression of some pluripotency genes was evaluated by RT-PCR and quantitative real-time PCR (qPCR) analyses. RESULTS: Third-passaged ADSCs showed a fibroblast-like morphology and expressed mesenchymal stem cell markers. They also showed adipogenic and osteogenic differentiation potential. QPCR analysis revealed a significant upregulation in the expression of some pluripotency genes including OCT4, SOX2, NANOG, REX1 and ESG1 in the reprogrammed ADSCs compared to the control group. CONCLUSION: These findings showed that mouse ESC extract can be used to induce reprogramming of human ADSCs. In fact, this method is applicable for reprogramming of human adult stem cells to a more pluripotent sate and may have a potential in regenerative medicine. Mashhad University of Medical Sciences 2017-11 /pmc/articles/PMC5749353/ /pubmed/29299196 http://dx.doi.org/10.22038/IJBMS.2017.9464 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Salehi, Paria Motamen Foroutan, Tahereh Javeri, Arash Taha, Masoumeh Fakhr Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
title | Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
title_full | Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
title_fullStr | Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
title_full_unstemmed | Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
title_short | Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
title_sort | extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749353/ https://www.ncbi.nlm.nih.gov/pubmed/29299196 http://dx.doi.org/10.22038/IJBMS.2017.9464 |
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