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Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750629/ https://www.ncbi.nlm.nih.gov/pubmed/29072582 http://dx.doi.org/10.3390/plants6040053 |
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author | Masiero, Eva Banik, Dipanwita Abson, John Greene, Paul Slater, Adrian Sgamma, Tiziana |
author_facet | Masiero, Eva Banik, Dipanwita Abson, John Greene, Paul Slater, Adrian Sgamma, Tiziana |
author_sort | Masiero, Eva |
collection | PubMed |
description | Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs. |
format | Online Article Text |
id | pubmed-5750629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57506292018-01-08 Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples Masiero, Eva Banik, Dipanwita Abson, John Greene, Paul Slater, Adrian Sgamma, Tiziana Plants (Basel) Article Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs. MDPI 2017-10-26 /pmc/articles/PMC5750629/ /pubmed/29072582 http://dx.doi.org/10.3390/plants6040053 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Masiero, Eva Banik, Dipanwita Abson, John Greene, Paul Slater, Adrian Sgamma, Tiziana Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples |
title | Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples |
title_full | Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples |
title_fullStr | Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples |
title_full_unstemmed | Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples |
title_short | Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples |
title_sort | genus-specific real-time pcr and hrm assays to distinguish liriope from ophiopogon samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750629/ https://www.ncbi.nlm.nih.gov/pubmed/29072582 http://dx.doi.org/10.3390/plants6040053 |
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