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Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples

Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera...

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Autores principales: Masiero, Eva, Banik, Dipanwita, Abson, John, Greene, Paul, Slater, Adrian, Sgamma, Tiziana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750629/
https://www.ncbi.nlm.nih.gov/pubmed/29072582
http://dx.doi.org/10.3390/plants6040053
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author Masiero, Eva
Banik, Dipanwita
Abson, John
Greene, Paul
Slater, Adrian
Sgamma, Tiziana
author_facet Masiero, Eva
Banik, Dipanwita
Abson, John
Greene, Paul
Slater, Adrian
Sgamma, Tiziana
author_sort Masiero, Eva
collection PubMed
description Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs.
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spelling pubmed-57506292018-01-08 Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples Masiero, Eva Banik, Dipanwita Abson, John Greene, Paul Slater, Adrian Sgamma, Tiziana Plants (Basel) Article Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs. MDPI 2017-10-26 /pmc/articles/PMC5750629/ /pubmed/29072582 http://dx.doi.org/10.3390/plants6040053 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Masiero, Eva
Banik, Dipanwita
Abson, John
Greene, Paul
Slater, Adrian
Sgamma, Tiziana
Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
title Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
title_full Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
title_fullStr Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
title_full_unstemmed Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
title_short Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
title_sort genus-specific real-time pcr and hrm assays to distinguish liriope from ophiopogon samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750629/
https://www.ncbi.nlm.nih.gov/pubmed/29072582
http://dx.doi.org/10.3390/plants6040053
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