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Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos

We report the biological functions of a zebrafish homologue of RING-finger protein 152 (rnf152) during embryogenesis. rnf152 was initially identified as a brain-enriched E3 ligase involved in early embryogenesis of zebrafish. Expression of rnf152 was ubiquitous in the brain at 24 hpf but restricted...

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Autores principales: Kumar, Ajeet, Huh, Tae-Lin, Choe, Joonho, Rhee, Myungchull
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750713/
https://www.ncbi.nlm.nih.gov/pubmed/29276941
http://dx.doi.org/10.14348/molcells.2017.0216
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author Kumar, Ajeet
Huh, Tae-Lin
Choe, Joonho
Rhee, Myungchull
author_facet Kumar, Ajeet
Huh, Tae-Lin
Choe, Joonho
Rhee, Myungchull
author_sort Kumar, Ajeet
collection PubMed
description We report the biological functions of a zebrafish homologue of RING-finger protein 152 (rnf152) during embryogenesis. rnf152 was initially identified as a brain-enriched E3 ligase involved in early embryogenesis of zebrafish. Expression of rnf152 was ubiquitous in the brain at 24 hpf but restricted to the eyes, midbrain-hindbrain boundary (MHB), and rhombomeres at 48 hpf. Knockdown of rnf152 in zebrafish embryos caused defects in the eyes, MHB, and rhombomeres (r1–7) at 24 hpf. These defects in rnf152-deficient embryos were analyzed by whole-mount in situ hybridization (WISH) using neuroD, deltaD, notch1a, and notch3 probes. NeuroD expression was abolished in the marginal zone, outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL) of the eyes at 27 hpf. Furthermore, deltaD and notch1a expression was remarkably reduced in the ONL, INL, subpallium, tectum, cerebellum, and rhombomeres (r1–7) at 24 hpf, whereas notch3 expression was reduced in the tectum, cerebellum, and rhombomeres at 24 hpf. Finally, we confirmed that expression of Notch target genes, her4 and ascl1a, also decreased significantly in these areas at 24 hpf. Thus, we propose that Rnf152 is essential for development of the eyes, midbrain and hindbrain, and that Delta-Notch signaling is involved.
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spelling pubmed-57507132018-01-19 Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos Kumar, Ajeet Huh, Tae-Lin Choe, Joonho Rhee, Myungchull Mol Cells Article We report the biological functions of a zebrafish homologue of RING-finger protein 152 (rnf152) during embryogenesis. rnf152 was initially identified as a brain-enriched E3 ligase involved in early embryogenesis of zebrafish. Expression of rnf152 was ubiquitous in the brain at 24 hpf but restricted to the eyes, midbrain-hindbrain boundary (MHB), and rhombomeres at 48 hpf. Knockdown of rnf152 in zebrafish embryos caused defects in the eyes, MHB, and rhombomeres (r1–7) at 24 hpf. These defects in rnf152-deficient embryos were analyzed by whole-mount in situ hybridization (WISH) using neuroD, deltaD, notch1a, and notch3 probes. NeuroD expression was abolished in the marginal zone, outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL) of the eyes at 27 hpf. Furthermore, deltaD and notch1a expression was remarkably reduced in the ONL, INL, subpallium, tectum, cerebellum, and rhombomeres (r1–7) at 24 hpf, whereas notch3 expression was reduced in the tectum, cerebellum, and rhombomeres at 24 hpf. Finally, we confirmed that expression of Notch target genes, her4 and ascl1a, also decreased significantly in these areas at 24 hpf. Thus, we propose that Rnf152 is essential for development of the eyes, midbrain and hindbrain, and that Delta-Notch signaling is involved. Korean Society for Molecular and Cellular Biology 2017-12-31 2017-12-22 /pmc/articles/PMC5750713/ /pubmed/29276941 http://dx.doi.org/10.14348/molcells.2017.0216 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Kumar, Ajeet
Huh, Tae-Lin
Choe, Joonho
Rhee, Myungchull
Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos
title Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos
title_full Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos
title_fullStr Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos
title_full_unstemmed Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos
title_short Rnf152 Is Essential for NeuroD Expression and Delta-Notch Signaling in the Zebrafish Embryos
title_sort rnf152 is essential for neurod expression and delta-notch signaling in the zebrafish embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750713/
https://www.ncbi.nlm.nih.gov/pubmed/29276941
http://dx.doi.org/10.14348/molcells.2017.0216
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