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An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study

Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that ar...

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Detalles Bibliográficos
Autores principales: Petrachi, Tiziana, Resca, Elisa, Piccinno, Maria Serena, Biagi, Francesco, Strusi, Valentina, Dominici, Massimo, Veronesi, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751004/
https://www.ncbi.nlm.nih.gov/pubmed/29258219
http://dx.doi.org/10.3390/ijerph14121587
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author Petrachi, Tiziana
Resca, Elisa
Piccinno, Maria Serena
Biagi, Francesco
Strusi, Valentina
Dominici, Massimo
Veronesi, Elena
author_facet Petrachi, Tiziana
Resca, Elisa
Piccinno, Maria Serena
Biagi, Francesco
Strusi, Valentina
Dominici, Massimo
Veronesi, Elena
author_sort Petrachi, Tiziana
collection PubMed
description Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method.
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spelling pubmed-57510042018-01-10 An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study Petrachi, Tiziana Resca, Elisa Piccinno, Maria Serena Biagi, Francesco Strusi, Valentina Dominici, Massimo Veronesi, Elena Int J Environ Res Public Health Communication Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method. MDPI 2017-12-17 2017-12 /pmc/articles/PMC5751004/ /pubmed/29258219 http://dx.doi.org/10.3390/ijerph14121587 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Petrachi, Tiziana
Resca, Elisa
Piccinno, Maria Serena
Biagi, Francesco
Strusi, Valentina
Dominici, Massimo
Veronesi, Elena
An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
title An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
title_full An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
title_fullStr An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
title_full_unstemmed An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
title_short An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
title_sort alternative approach to investigate biofilm in medical devices: a feasibility study
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751004/
https://www.ncbi.nlm.nih.gov/pubmed/29258219
http://dx.doi.org/10.3390/ijerph14121587
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