Cargando…
An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study
Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that ar...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751004/ https://www.ncbi.nlm.nih.gov/pubmed/29258219 http://dx.doi.org/10.3390/ijerph14121587 |
_version_ | 1783289850654359552 |
---|---|
author | Petrachi, Tiziana Resca, Elisa Piccinno, Maria Serena Biagi, Francesco Strusi, Valentina Dominici, Massimo Veronesi, Elena |
author_facet | Petrachi, Tiziana Resca, Elisa Piccinno, Maria Serena Biagi, Francesco Strusi, Valentina Dominici, Massimo Veronesi, Elena |
author_sort | Petrachi, Tiziana |
collection | PubMed |
description | Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method. |
format | Online Article Text |
id | pubmed-5751004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57510042018-01-10 An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study Petrachi, Tiziana Resca, Elisa Piccinno, Maria Serena Biagi, Francesco Strusi, Valentina Dominici, Massimo Veronesi, Elena Int J Environ Res Public Health Communication Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method. MDPI 2017-12-17 2017-12 /pmc/articles/PMC5751004/ /pubmed/29258219 http://dx.doi.org/10.3390/ijerph14121587 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Petrachi, Tiziana Resca, Elisa Piccinno, Maria Serena Biagi, Francesco Strusi, Valentina Dominici, Massimo Veronesi, Elena An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study |
title | An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study |
title_full | An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study |
title_fullStr | An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study |
title_full_unstemmed | An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study |
title_short | An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study |
title_sort | alternative approach to investigate biofilm in medical devices: a feasibility study |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751004/ https://www.ncbi.nlm.nih.gov/pubmed/29258219 http://dx.doi.org/10.3390/ijerph14121587 |
work_keys_str_mv | AT petrachitiziana analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT rescaelisa analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT piccinnomariaserena analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT biagifrancesco analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT strusivalentina analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT dominicimassimo analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT veronesielena analternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT petrachitiziana alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT rescaelisa alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT piccinnomariaserena alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT biagifrancesco alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT strusivalentina alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT dominicimassimo alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy AT veronesielena alternativeapproachtoinvestigatebiofilminmedicaldevicesafeasibilitystudy |