Cargando…

The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling

Civilization diseases associated with memory disorders are important health problems occurring due to a prolonged life span. The manuscript shows the results of an in vivo study targeting the emergence of two drug candidates with anti-amnestic properties. The preceding quantitative structure–activit...

Descripción completa

Detalles Bibliográficos
Autores principales: Kukula-Koch, Wirginia, Kruk-Słomka, Marta, Stępnik, Katarzyna, Szalak, Radosław, Biała, Grażyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751114/
https://www.ncbi.nlm.nih.gov/pubmed/29186770
http://dx.doi.org/10.3390/ijms18122511
_version_ 1783289876101201920
author Kukula-Koch, Wirginia
Kruk-Słomka, Marta
Stępnik, Katarzyna
Szalak, Radosław
Biała, Grażyna
author_facet Kukula-Koch, Wirginia
Kruk-Słomka, Marta
Stępnik, Katarzyna
Szalak, Radosław
Biała, Grażyna
author_sort Kukula-Koch, Wirginia
collection PubMed
description Civilization diseases associated with memory disorders are important health problems occurring due to a prolonged life span. The manuscript shows the results of an in vivo study targeting the emergence of two drug candidates with anti-amnestic properties. The preceding quantitative structure–activity relationship (QSAR) studies provided information on the ability of berberine and magnoflorine to cross the blood–brain barrier (BBB). In the light of these findings, both compounds were purified from crude plant extracts of barberries: berberine—from Berberis siberica using a method published earlier, and magnoflorine—from Berberis cretica by centrifugal partition chromatography (solvent system: ethyl acetate:butanol:water-0.6:1.5:3 v/v/v). Both the compounds were evaluated for their memory enhancing and scopolamine inhibitory properties in an in vivo passive avoidance (PA) test on mice towards short-term and long-term memory. Cognition enhancing properties were observed at the following doses: 5 mg/kg (i.p.) for berberine and 20 mg/kg (i.p.) for magnoflorine. In addition, both the tested isoquinolines with the co-administered scopolamine were found to block long-term but not short-term memory impairment. No influence on the locomotor activity was observed for the tested doses. The results confirmed a marked central activity of magnoflorine and showed the necessity to lower the dosage of berberine. Optimized purification conditions have been elaborated for magnoflorine.
format Online
Article
Text
id pubmed-5751114
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57511142018-01-08 The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling Kukula-Koch, Wirginia Kruk-Słomka, Marta Stępnik, Katarzyna Szalak, Radosław Biała, Grażyna Int J Mol Sci Article Civilization diseases associated with memory disorders are important health problems occurring due to a prolonged life span. The manuscript shows the results of an in vivo study targeting the emergence of two drug candidates with anti-amnestic properties. The preceding quantitative structure–activity relationship (QSAR) studies provided information on the ability of berberine and magnoflorine to cross the blood–brain barrier (BBB). In the light of these findings, both compounds were purified from crude plant extracts of barberries: berberine—from Berberis siberica using a method published earlier, and magnoflorine—from Berberis cretica by centrifugal partition chromatography (solvent system: ethyl acetate:butanol:water-0.6:1.5:3 v/v/v). Both the compounds were evaluated for their memory enhancing and scopolamine inhibitory properties in an in vivo passive avoidance (PA) test on mice towards short-term and long-term memory. Cognition enhancing properties were observed at the following doses: 5 mg/kg (i.p.) for berberine and 20 mg/kg (i.p.) for magnoflorine. In addition, both the tested isoquinolines with the co-administered scopolamine were found to block long-term but not short-term memory impairment. No influence on the locomotor activity was observed for the tested doses. The results confirmed a marked central activity of magnoflorine and showed the necessity to lower the dosage of berberine. Optimized purification conditions have been elaborated for magnoflorine. MDPI 2017-11-24 /pmc/articles/PMC5751114/ /pubmed/29186770 http://dx.doi.org/10.3390/ijms18122511 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kukula-Koch, Wirginia
Kruk-Słomka, Marta
Stępnik, Katarzyna
Szalak, Radosław
Biała, Grażyna
The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling
title The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling
title_full The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling
title_fullStr The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling
title_full_unstemmed The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling
title_short The Evaluation of Pro-Cognitive and Antiamnestic Properties of Berberine and Magnoflorine Isolated from Barberry Species by Centrifugal Partition Chromatography (CPC), in Relation to QSAR Modelling
title_sort evaluation of pro-cognitive and antiamnestic properties of berberine and magnoflorine isolated from barberry species by centrifugal partition chromatography (cpc), in relation to qsar modelling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751114/
https://www.ncbi.nlm.nih.gov/pubmed/29186770
http://dx.doi.org/10.3390/ijms18122511
work_keys_str_mv AT kukulakochwirginia theevaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT kruksłomkamarta theevaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT stepnikkatarzyna theevaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT szalakradosław theevaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT białagrazyna theevaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT kukulakochwirginia evaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT kruksłomkamarta evaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT stepnikkatarzyna evaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT szalakradosław evaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling
AT białagrazyna evaluationofprocognitiveandantiamnesticpropertiesofberberineandmagnoflorineisolatedfrombarberryspeciesbycentrifugalpartitionchromatographycpcinrelationtoqsarmodelling