Cargando…

Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo

Nitric oxide (NO) is involved in several biological processes, but its role in human melanogenesis is still not well understood. Exposure to UVA and UVB induces nitric oxide production in keratinocytes and melanocytes through the activation of constitutive nitric oxide synthase, increasing tyrosinas...

Descripción completa

Detalles Bibliográficos
Autores principales: Vaccaro, Mario, Irrera, Natasha, Cutroneo, Giuseppina, Rizzo, Giuseppina, Vaccaro, Federico, Anastasi, Giuseppe P., Borgia, Francesco, Cannavò, Serafinella P., Altavilla, Domenica, Squadrito, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751136/
https://www.ncbi.nlm.nih.gov/pubmed/29186858
http://dx.doi.org/10.3390/ijms18122533
_version_ 1783289881242370048
author Vaccaro, Mario
Irrera, Natasha
Cutroneo, Giuseppina
Rizzo, Giuseppina
Vaccaro, Federico
Anastasi, Giuseppe P.
Borgia, Francesco
Cannavò, Serafinella P.
Altavilla, Domenica
Squadrito, Francesco
author_facet Vaccaro, Mario
Irrera, Natasha
Cutroneo, Giuseppina
Rizzo, Giuseppina
Vaccaro, Federico
Anastasi, Giuseppe P.
Borgia, Francesco
Cannavò, Serafinella P.
Altavilla, Domenica
Squadrito, Francesco
author_sort Vaccaro, Mario
collection PubMed
description Nitric oxide (NO) is involved in several biological processes, but its role in human melanogenesis is still not well understood. Exposure to UVA and UVB induces nitric oxide production in keratinocytes and melanocytes through the activation of constitutive nitric oxide synthase, increasing tyrosinase activity and melanin synthesis, whereas inducible nitric oxide synthase over expression might be involved in hypopigmentary disorders. The aim of this study was to evaluate whether inducible nitric oxide synthase and neuronal nitric oxide synthase expression were modified in vitiligo skin compared to healthy controls. Skin biopsies were obtained from inflammatory/lesional and white/lesional skin in 12 patients with active, non-segmental vitiligo; site-matched biopsies of normal skin from eight patients were used as controls. Nitric oxide synthase isoforms expression was evaluated by confocal laser scanning microscopy and Western Blot analysis. Inducible nitric oxide synthase expression was significantly increased in inflammatory/lesional skin compared to healthy skin; melanocytes showed a moderate neuronal nitric oxide synthase expression in white/lesional skin, demonstrating that metabolic function still goes on. The obtained data demonstrated that vitiligo lesions were characterized by modifications of nitric oxide synthase isoforms, thus confirming the hypothesis that nitric oxide imbalance is involved in vitiligo and supporting the idea that nitric oxide synthase inhibitors might be used as a possible therapeutic approach for the management of vitiligo.
format Online
Article
Text
id pubmed-5751136
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57511362018-01-08 Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo Vaccaro, Mario Irrera, Natasha Cutroneo, Giuseppina Rizzo, Giuseppina Vaccaro, Federico Anastasi, Giuseppe P. Borgia, Francesco Cannavò, Serafinella P. Altavilla, Domenica Squadrito, Francesco Int J Mol Sci Article Nitric oxide (NO) is involved in several biological processes, but its role in human melanogenesis is still not well understood. Exposure to UVA and UVB induces nitric oxide production in keratinocytes and melanocytes through the activation of constitutive nitric oxide synthase, increasing tyrosinase activity and melanin synthesis, whereas inducible nitric oxide synthase over expression might be involved in hypopigmentary disorders. The aim of this study was to evaluate whether inducible nitric oxide synthase and neuronal nitric oxide synthase expression were modified in vitiligo skin compared to healthy controls. Skin biopsies were obtained from inflammatory/lesional and white/lesional skin in 12 patients with active, non-segmental vitiligo; site-matched biopsies of normal skin from eight patients were used as controls. Nitric oxide synthase isoforms expression was evaluated by confocal laser scanning microscopy and Western Blot analysis. Inducible nitric oxide synthase expression was significantly increased in inflammatory/lesional skin compared to healthy skin; melanocytes showed a moderate neuronal nitric oxide synthase expression in white/lesional skin, demonstrating that metabolic function still goes on. The obtained data demonstrated that vitiligo lesions were characterized by modifications of nitric oxide synthase isoforms, thus confirming the hypothesis that nitric oxide imbalance is involved in vitiligo and supporting the idea that nitric oxide synthase inhibitors might be used as a possible therapeutic approach for the management of vitiligo. MDPI 2017-11-26 /pmc/articles/PMC5751136/ /pubmed/29186858 http://dx.doi.org/10.3390/ijms18122533 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vaccaro, Mario
Irrera, Natasha
Cutroneo, Giuseppina
Rizzo, Giuseppina
Vaccaro, Federico
Anastasi, Giuseppe P.
Borgia, Francesco
Cannavò, Serafinella P.
Altavilla, Domenica
Squadrito, Francesco
Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo
title Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo
title_full Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo
title_fullStr Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo
title_full_unstemmed Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo
title_short Differential Expression of Nitric Oxide Synthase Isoforms nNOS and iNOS in Patients with Non-Segmental Generalized Vitiligo
title_sort differential expression of nitric oxide synthase isoforms nnos and inos in patients with non-segmental generalized vitiligo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751136/
https://www.ncbi.nlm.nih.gov/pubmed/29186858
http://dx.doi.org/10.3390/ijms18122533
work_keys_str_mv AT vaccaromario differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT irreranatasha differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT cutroneogiuseppina differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT rizzogiuseppina differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT vaccarofederico differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT anastasigiuseppep differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT borgiafrancesco differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT cannavoserafinellap differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT altavilladomenica differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo
AT squadritofrancesco differentialexpressionofnitricoxidesynthaseisoformsnnosandinosinpatientswithnonsegmentalgeneralizedvitiligo