Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System

The recent advancement in genome editing such a CRISPR/Cas9 system has enabled isolation of cells with knocked multiple alleles through a one-step transfection. Somatic cell nuclear transfer (SCNT) has been frequently employed as one of the efficient tools for the production of genetically modified...

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Detalles Bibliográficos
Autores principales: Sato, Masahiro, Miyoshi, Kazuchika, Nakamura, Shingo, Ohtsuka, Masato, Sakurai, Takayuki, Watanabe, Satoshi, Kawaguchi, Hiroaki, Tanimoto, Akihide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751213/
https://www.ncbi.nlm.nih.gov/pubmed/29207527
http://dx.doi.org/10.3390/ijms18122610

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