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Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures

The elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the inter...

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Autores principales: Nealon, John Oliver, Philomina, Limcy Seby, McGuffin, Liam James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751226/
https://www.ncbi.nlm.nih.gov/pubmed/29206185
http://dx.doi.org/10.3390/ijms18122623
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author Nealon, John Oliver
Philomina, Limcy Seby
McGuffin, Liam James
author_facet Nealon, John Oliver
Philomina, Limcy Seby
McGuffin, Liam James
author_sort Nealon, John Oliver
collection PubMed
description The elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the interacting partner proteins of predicted protein structures is very time-consuming when using in vitro methods, this can be somewhat alleviated by use of predictive methods. However, developing reliably accurate predictive tools has proved to be difficult. We review the current state of the art in predictive protein interaction software and discuss the problem of scoring and therefore ranking predictions. Current community-based predictive exercises are discussed in relation to the growth of protein interaction prediction as an area within these exercises. We suggest a fusion of experimental and predictive methods that make use of sparse experimental data to determine higher resolution predicted protein interactions as being necessary to drive forward development.
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spelling pubmed-57512262018-01-08 Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures Nealon, John Oliver Philomina, Limcy Seby McGuffin, Liam James Int J Mol Sci Review The elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the interacting partner proteins of predicted protein structures is very time-consuming when using in vitro methods, this can be somewhat alleviated by use of predictive methods. However, developing reliably accurate predictive tools has proved to be difficult. We review the current state of the art in predictive protein interaction software and discuss the problem of scoring and therefore ranking predictions. Current community-based predictive exercises are discussed in relation to the growth of protein interaction prediction as an area within these exercises. We suggest a fusion of experimental and predictive methods that make use of sparse experimental data to determine higher resolution predicted protein interactions as being necessary to drive forward development. MDPI 2017-12-05 /pmc/articles/PMC5751226/ /pubmed/29206185 http://dx.doi.org/10.3390/ijms18122623 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Nealon, John Oliver
Philomina, Limcy Seby
McGuffin, Liam James
Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
title Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
title_full Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
title_fullStr Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
title_full_unstemmed Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
title_short Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures
title_sort predictive and experimental approaches for elucidating protein–protein interactions and quaternary structures
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751226/
https://www.ncbi.nlm.nih.gov/pubmed/29206185
http://dx.doi.org/10.3390/ijms18122623
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