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Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway

Marine algae possess a variety of beneficial effects on human health. In this study, we investigated whether diphlorethohydroxycarmalol (DPHC), isolated from Ishige okamurae, a brown alga, suppresses receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. DPHC sign...

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Autores principales: Ihn, Hye Jung, Kim, Ju Ang, Cho, Hye Sung, Shin, Hong-In, Kim, Gi-Young, Choi, Yung Hyun, Jeon, You-Jin, Park, Eui Kyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751238/
https://www.ncbi.nlm.nih.gov/pubmed/29211036
http://dx.doi.org/10.3390/ijms18122635
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author Ihn, Hye Jung
Kim, Ju Ang
Cho, Hye Sung
Shin, Hong-In
Kim, Gi-Young
Choi, Yung Hyun
Jeon, You-Jin
Park, Eui Kyun
author_facet Ihn, Hye Jung
Kim, Ju Ang
Cho, Hye Sung
Shin, Hong-In
Kim, Gi-Young
Choi, Yung Hyun
Jeon, You-Jin
Park, Eui Kyun
author_sort Ihn, Hye Jung
collection PubMed
description Marine algae possess a variety of beneficial effects on human health. In this study, we investigated whether diphlorethohydroxycarmalol (DPHC), isolated from Ishige okamurae, a brown alga, suppresses receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. DPHC significantly suppressed RANKL-induced osteoclast differentiation and macrophage-colony stimulating factor (M-CSF) expression in a dose-dependent manner. In addition, it significantly inhibited actin ring formation, the expression of osteoclast marker genes, such as tartrate-resistant acid phosphatase (TRAP), nuclear factor of activated T-cells cytoplasmic 1 (Nfatc1), cathepsin K (Ctsk), and dendritic cell-specific transmembrane protein (Dcstamp), and osteoclast-induced bone resorption. Analysis of the RANKL-mediated signaling pathway showed that the phosphorylation of both IκB and p65 was specifically inhibited by DPHC. These results suggest that DPHC substantially suppresses osteoclastogenesis by downregulating the RANK-NF-κB signaling pathway. Thus, it holds significant potential for the treatment of skeletal diseases associated with an enhanced osteoclast activity.
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spelling pubmed-57512382018-01-08 Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway Ihn, Hye Jung Kim, Ju Ang Cho, Hye Sung Shin, Hong-In Kim, Gi-Young Choi, Yung Hyun Jeon, You-Jin Park, Eui Kyun Int J Mol Sci Article Marine algae possess a variety of beneficial effects on human health. In this study, we investigated whether diphlorethohydroxycarmalol (DPHC), isolated from Ishige okamurae, a brown alga, suppresses receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. DPHC significantly suppressed RANKL-induced osteoclast differentiation and macrophage-colony stimulating factor (M-CSF) expression in a dose-dependent manner. In addition, it significantly inhibited actin ring formation, the expression of osteoclast marker genes, such as tartrate-resistant acid phosphatase (TRAP), nuclear factor of activated T-cells cytoplasmic 1 (Nfatc1), cathepsin K (Ctsk), and dendritic cell-specific transmembrane protein (Dcstamp), and osteoclast-induced bone resorption. Analysis of the RANKL-mediated signaling pathway showed that the phosphorylation of both IκB and p65 was specifically inhibited by DPHC. These results suggest that DPHC substantially suppresses osteoclastogenesis by downregulating the RANK-NF-κB signaling pathway. Thus, it holds significant potential for the treatment of skeletal diseases associated with an enhanced osteoclast activity. MDPI 2017-12-06 /pmc/articles/PMC5751238/ /pubmed/29211036 http://dx.doi.org/10.3390/ijms18122635 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ihn, Hye Jung
Kim, Ju Ang
Cho, Hye Sung
Shin, Hong-In
Kim, Gi-Young
Choi, Yung Hyun
Jeon, You-Jin
Park, Eui Kyun
Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway
title Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway
title_full Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway
title_fullStr Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway
title_full_unstemmed Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway
title_short Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway
title_sort diphlorethohydroxycarmalol from ishige okamurae suppresses osteoclast differentiation by downregulating the nf-κb signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751238/
https://www.ncbi.nlm.nih.gov/pubmed/29211036
http://dx.doi.org/10.3390/ijms18122635
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