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In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages

Recent studies have shown promising results concerning the effectiveness of 3D plates in terms of stabilization of condylar fractures. Despite the use of new techniques and new materials, we can still observe certain side effects, including the immune reaction of the body, which may lead to the exce...

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Autores principales: Sikora, Maciej, Goschorska, Marta, Baranowska-Bosiacka, Irena, Chlubek, Dariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751241/
https://www.ncbi.nlm.nih.gov/pubmed/29292766
http://dx.doi.org/10.3390/ijms18122638
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author Sikora, Maciej
Goschorska, Marta
Baranowska-Bosiacka, Irena
Chlubek, Dariusz
author_facet Sikora, Maciej
Goschorska, Marta
Baranowska-Bosiacka, Irena
Chlubek, Dariusz
author_sort Sikora, Maciej
collection PubMed
description Recent studies have shown promising results concerning the effectiveness of 3D plates in terms of stabilization of condylar fractures. Despite the use of new techniques and new materials, we can still observe certain side effects, including the immune reaction of the body, which may lead to the excessive inflammation. The aim of this paper was to determine how the production of prostaglandin E(2) (PGE(2)) and thromboxane B(2) (TXB(2)) in THP-1 monocytes/macrophages is influenced by the titanium 3D plates and dedicated screws. The experiments were conducted on THP-1 monocytic cell line and macrophages derived from a THP-1cells. The concentrations of PGE(2) and TXB(2) released were measured by using immunoassay kit. Verification of plate-induced activation of THP-1 monocytes and macrophages and initiation of inflammatory reaction was conducted by flow cytometry. Despite some differences in the content of the implant devices our results showed that these plates did not statistically significantly increase the production of these prostanoids. Osteosynthesis of condylar fractures using 3D titanium mini-plates seems to be a good alternative to traditional plates due to their lack of stimulating the cyclooxygenase-dependent production of prostanoids; limiting the development of inflammatory reactions.
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spelling pubmed-57512412018-01-08 In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages Sikora, Maciej Goschorska, Marta Baranowska-Bosiacka, Irena Chlubek, Dariusz Int J Mol Sci Article Recent studies have shown promising results concerning the effectiveness of 3D plates in terms of stabilization of condylar fractures. Despite the use of new techniques and new materials, we can still observe certain side effects, including the immune reaction of the body, which may lead to the excessive inflammation. The aim of this paper was to determine how the production of prostaglandin E(2) (PGE(2)) and thromboxane B(2) (TXB(2)) in THP-1 monocytes/macrophages is influenced by the titanium 3D plates and dedicated screws. The experiments were conducted on THP-1 monocytic cell line and macrophages derived from a THP-1cells. The concentrations of PGE(2) and TXB(2) released were measured by using immunoassay kit. Verification of plate-induced activation of THP-1 monocytes and macrophages and initiation of inflammatory reaction was conducted by flow cytometry. Despite some differences in the content of the implant devices our results showed that these plates did not statistically significantly increase the production of these prostanoids. Osteosynthesis of condylar fractures using 3D titanium mini-plates seems to be a good alternative to traditional plates due to their lack of stimulating the cyclooxygenase-dependent production of prostanoids; limiting the development of inflammatory reactions. MDPI 2017-12-08 /pmc/articles/PMC5751241/ /pubmed/29292766 http://dx.doi.org/10.3390/ijms18122638 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sikora, Maciej
Goschorska, Marta
Baranowska-Bosiacka, Irena
Chlubek, Dariusz
In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages
title In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages
title_full In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages
title_fullStr In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages
title_full_unstemmed In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages
title_short In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E(2) (PGE(2)) and Thromboxane B(2) (TXB(2)) Concentration in THP-1 Macrophages
title_sort in vitro effect of 3d plates used for surgical treatment of condylar fractures on prostaglandin e(2) (pge(2)) and thromboxane b(2) (txb(2)) concentration in thp-1 macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751241/
https://www.ncbi.nlm.nih.gov/pubmed/29292766
http://dx.doi.org/10.3390/ijms18122638
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