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Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis

Myocarditis is an inflammatory disease of the myocardium that causes cardiogenic shock and death. However, endomyocardial biopsy that is, the gold standard for a diagnosis is limited. Apurinic/apyrimidinic endonuclease 1/redox effector factor-1 (APE1/Ref-1) is a multifunctional protein, which is inv...

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Autores principales: Jin, Seon-Ah, Lim, Byung-Kwan, Seo, Hee Jung, Kim, Sun Kyeong, Ahn, Kye Taek, Jeon, Byeong Hwa, Jeong, Jin-Ok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751266/
https://www.ncbi.nlm.nih.gov/pubmed/29292734
http://dx.doi.org/10.3390/ijms18122664
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author Jin, Seon-Ah
Lim, Byung-Kwan
Seo, Hee Jung
Kim, Sun Kyeong
Ahn, Kye Taek
Jeon, Byeong Hwa
Jeong, Jin-Ok
author_facet Jin, Seon-Ah
Lim, Byung-Kwan
Seo, Hee Jung
Kim, Sun Kyeong
Ahn, Kye Taek
Jeon, Byeong Hwa
Jeong, Jin-Ok
author_sort Jin, Seon-Ah
collection PubMed
description Myocarditis is an inflammatory disease of the myocardium that causes cardiogenic shock and death. However, endomyocardial biopsy that is, the gold standard for a diagnosis is limited. Apurinic/apyrimidinic endonuclease 1/redox effector factor-1 (APE1/Ref-1) is a multifunctional protein, which is involved in DNA-based excision repair pathway, and in redox signaling, its changes are observed in various cardiovascular diseases including hypertension and coronary artery disease. We analyzed serum APE1/Ref-1 in experimental murine myocarditis. To induce myocarditis, coxsackievirus B3 was injected intraperitoneally to BALB/c mice. The serum APE1/Ref-1, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and troponin I were measured. The histology and virus titers measurements were performed. The troponin I and inflammation were significantly elevated at day 3, peaked to day 7 and decreased at day 10. The NT-proBNP and virus titers were significantly peaked at day 3, and dropped at day 7 and 10. The serum APE1/Ref-1 was gradually raised and its elevation is still maintained until a later time, namely day 10. Also, its level was positively correlated with myocardial inflammation, reflecting severity of myocardial injury. We suggest that serum APE1/Ref-1 can be used to assess for myocardial injury in viral myocarditis without endomyocardial biopsy.
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spelling pubmed-57512662018-01-08 Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis Jin, Seon-Ah Lim, Byung-Kwan Seo, Hee Jung Kim, Sun Kyeong Ahn, Kye Taek Jeon, Byeong Hwa Jeong, Jin-Ok Int J Mol Sci Article Myocarditis is an inflammatory disease of the myocardium that causes cardiogenic shock and death. However, endomyocardial biopsy that is, the gold standard for a diagnosis is limited. Apurinic/apyrimidinic endonuclease 1/redox effector factor-1 (APE1/Ref-1) is a multifunctional protein, which is involved in DNA-based excision repair pathway, and in redox signaling, its changes are observed in various cardiovascular diseases including hypertension and coronary artery disease. We analyzed serum APE1/Ref-1 in experimental murine myocarditis. To induce myocarditis, coxsackievirus B3 was injected intraperitoneally to BALB/c mice. The serum APE1/Ref-1, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and troponin I were measured. The histology and virus titers measurements were performed. The troponin I and inflammation were significantly elevated at day 3, peaked to day 7 and decreased at day 10. The NT-proBNP and virus titers were significantly peaked at day 3, and dropped at day 7 and 10. The serum APE1/Ref-1 was gradually raised and its elevation is still maintained until a later time, namely day 10. Also, its level was positively correlated with myocardial inflammation, reflecting severity of myocardial injury. We suggest that serum APE1/Ref-1 can be used to assess for myocardial injury in viral myocarditis without endomyocardial biopsy. MDPI 2017-12-08 /pmc/articles/PMC5751266/ /pubmed/29292734 http://dx.doi.org/10.3390/ijms18122664 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jin, Seon-Ah
Lim, Byung-Kwan
Seo, Hee Jung
Kim, Sun Kyeong
Ahn, Kye Taek
Jeon, Byeong Hwa
Jeong, Jin-Ok
Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis
title Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis
title_full Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis
title_fullStr Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis
title_full_unstemmed Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis
title_short Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis
title_sort elevation of serum ape1/ref-1 in experimental murine myocarditis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751266/
https://www.ncbi.nlm.nih.gov/pubmed/29292734
http://dx.doi.org/10.3390/ijms18122664
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