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Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels

Given the importance of the extracellular medium during tissue formation, it was wise to develop an artificial structure that mimics the extracellular matrix while having improved physico-chemical properties. That is why the choice was focused on gelatin methacryloyl (GelMA), an inexpensive biocompa...

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Autores principales: Rahali, Kamel, Ben Messaoud, Ghazi, Kahn, Cyril J.F., Sanchez-Gonzalez, Laura, Kaci, Mouna, Cleymand, Franck, Fleutot, Solenne, Linder, Michel, Desobry, Stéphane, Arab-Tehrany, Elmira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751277/
https://www.ncbi.nlm.nih.gov/pubmed/29232870
http://dx.doi.org/10.3390/ijms18122675
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author Rahali, Kamel
Ben Messaoud, Ghazi
Kahn, Cyril J.F.
Sanchez-Gonzalez, Laura
Kaci, Mouna
Cleymand, Franck
Fleutot, Solenne
Linder, Michel
Desobry, Stéphane
Arab-Tehrany, Elmira
author_facet Rahali, Kamel
Ben Messaoud, Ghazi
Kahn, Cyril J.F.
Sanchez-Gonzalez, Laura
Kaci, Mouna
Cleymand, Franck
Fleutot, Solenne
Linder, Michel
Desobry, Stéphane
Arab-Tehrany, Elmira
author_sort Rahali, Kamel
collection PubMed
description Given the importance of the extracellular medium during tissue formation, it was wise to develop an artificial structure that mimics the extracellular matrix while having improved physico-chemical properties. That is why the choice was focused on gelatin methacryloyl (GelMA), an inexpensive biocompatible hydrogel. Physicochemical and mechanical properties were improved by the incorporation of nanoparticles developed from two innovative fabrication processes: High shear fluid and low frequencies/high frequencies ultrasounds. Both rapeseed nanoliposomes and nanodroplets were successfully incorporated in the GelMA networks during the photo polymerization process. The impact on polymer microstructure was investigated by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and enzymatic degradation investigations. Mechanical stability and viscoelastic tests were conducted to demonstrate the beneficial effect of the functionalization on GelMA hydrogels. Adding nanoparticles to GelMA improved the surface properties (porosity), tuned swelling, and degradability properties. In addition, we observed that nanoemulsion didn’t change significantly the mechanical properties to shear and compression solicitations, whereas nanoliposome addition decreased Young’s modulus under compression solicitations. Thus, these ways of functionalization allow controlling the design of the material by choosing the type of nanoparticle (nanoliposome or nanoemulsion) in function of the application.
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spelling pubmed-57512772018-01-08 Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels Rahali, Kamel Ben Messaoud, Ghazi Kahn, Cyril J.F. Sanchez-Gonzalez, Laura Kaci, Mouna Cleymand, Franck Fleutot, Solenne Linder, Michel Desobry, Stéphane Arab-Tehrany, Elmira Int J Mol Sci Article Given the importance of the extracellular medium during tissue formation, it was wise to develop an artificial structure that mimics the extracellular matrix while having improved physico-chemical properties. That is why the choice was focused on gelatin methacryloyl (GelMA), an inexpensive biocompatible hydrogel. Physicochemical and mechanical properties were improved by the incorporation of nanoparticles developed from two innovative fabrication processes: High shear fluid and low frequencies/high frequencies ultrasounds. Both rapeseed nanoliposomes and nanodroplets were successfully incorporated in the GelMA networks during the photo polymerization process. The impact on polymer microstructure was investigated by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and enzymatic degradation investigations. Mechanical stability and viscoelastic tests were conducted to demonstrate the beneficial effect of the functionalization on GelMA hydrogels. Adding nanoparticles to GelMA improved the surface properties (porosity), tuned swelling, and degradability properties. In addition, we observed that nanoemulsion didn’t change significantly the mechanical properties to shear and compression solicitations, whereas nanoliposome addition decreased Young’s modulus under compression solicitations. Thus, these ways of functionalization allow controlling the design of the material by choosing the type of nanoparticle (nanoliposome or nanoemulsion) in function of the application. MDPI 2017-12-10 /pmc/articles/PMC5751277/ /pubmed/29232870 http://dx.doi.org/10.3390/ijms18122675 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rahali, Kamel
Ben Messaoud, Ghazi
Kahn, Cyril J.F.
Sanchez-Gonzalez, Laura
Kaci, Mouna
Cleymand, Franck
Fleutot, Solenne
Linder, Michel
Desobry, Stéphane
Arab-Tehrany, Elmira
Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
title Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
title_full Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
title_fullStr Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
title_full_unstemmed Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
title_short Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
title_sort synthesis and characterization of nanofunctionalized gelatin methacrylate hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751277/
https://www.ncbi.nlm.nih.gov/pubmed/29232870
http://dx.doi.org/10.3390/ijms18122675
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