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Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors

Serotonin is an important neurotransmitter that plays a major role in the pathogenesis of a variety of conditions, including psychiatric disorders. The detection of serotonin typically relies on high-performance liquid chromatography (HPLC), an expensive technique that requires sophisticated equipme...

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Autores principales: Vandenryt, Thijs, van Grinsven, Bart, Eersels, Kasper, Cornelis, Peter, Kholwadia, Safira, Cleij, Thomas J., Thoelen, Ronald, De Ceuninck, Ward, Peeters, Marloes, Wagner, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751640/
https://www.ncbi.nlm.nih.gov/pubmed/29168733
http://dx.doi.org/10.3390/s17122701
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author Vandenryt, Thijs
van Grinsven, Bart
Eersels, Kasper
Cornelis, Peter
Kholwadia, Safira
Cleij, Thomas J.
Thoelen, Ronald
De Ceuninck, Ward
Peeters, Marloes
Wagner, Patrick
author_facet Vandenryt, Thijs
van Grinsven, Bart
Eersels, Kasper
Cornelis, Peter
Kholwadia, Safira
Cleij, Thomas J.
Thoelen, Ronald
De Ceuninck, Ward
Peeters, Marloes
Wagner, Patrick
author_sort Vandenryt, Thijs
collection PubMed
description Serotonin is an important neurotransmitter that plays a major role in the pathogenesis of a variety of conditions, including psychiatric disorders. The detection of serotonin typically relies on high-performance liquid chromatography (HPLC), an expensive technique that requires sophisticated equipment and trained personnel, and is not suitable for point-of-care applications. In this contribution, we introduce a novel sensor platform that can measure spiked neurotransmitter concentrations in whole blood samples in a fast and low-cost manner by combining synthetic receptors with a thermal readout technique—the heat-transfer method. In addition, the design of a miniaturized version of the sensing platform is presented that aims to bridge the gap between measurements in a laboratory setting and point-of-care measurements. This fully automated and integrated, user-friendly design features a capillary pumping unit that is compatible with point-of-care sampling techniques such as a blood lancet device (sample volume—between 50 µL and 300 µL). Sample pre-treatment is limited to the addition of an anti-coagulant. With this fully integrated setup, it is possible to successfully discriminate serotonin from a competitor neurotransmitter (histamine) in whole blood samples. This is the first demonstration of a point-of-care ready device based on synthetic receptors for the screening of neurotransmitters in complex matrices, illustrating the sensor’s potential application in clinical research and diagnosis of e.g., early stage depression.
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spelling pubmed-57516402018-01-10 Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors Vandenryt, Thijs van Grinsven, Bart Eersels, Kasper Cornelis, Peter Kholwadia, Safira Cleij, Thomas J. Thoelen, Ronald De Ceuninck, Ward Peeters, Marloes Wagner, Patrick Sensors (Basel) Article Serotonin is an important neurotransmitter that plays a major role in the pathogenesis of a variety of conditions, including psychiatric disorders. The detection of serotonin typically relies on high-performance liquid chromatography (HPLC), an expensive technique that requires sophisticated equipment and trained personnel, and is not suitable for point-of-care applications. In this contribution, we introduce a novel sensor platform that can measure spiked neurotransmitter concentrations in whole blood samples in a fast and low-cost manner by combining synthetic receptors with a thermal readout technique—the heat-transfer method. In addition, the design of a miniaturized version of the sensing platform is presented that aims to bridge the gap between measurements in a laboratory setting and point-of-care measurements. This fully automated and integrated, user-friendly design features a capillary pumping unit that is compatible with point-of-care sampling techniques such as a blood lancet device (sample volume—between 50 µL and 300 µL). Sample pre-treatment is limited to the addition of an anti-coagulant. With this fully integrated setup, it is possible to successfully discriminate serotonin from a competitor neurotransmitter (histamine) in whole blood samples. This is the first demonstration of a point-of-care ready device based on synthetic receptors for the screening of neurotransmitters in complex matrices, illustrating the sensor’s potential application in clinical research and diagnosis of e.g., early stage depression. MDPI 2017-11-23 /pmc/articles/PMC5751640/ /pubmed/29168733 http://dx.doi.org/10.3390/s17122701 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vandenryt, Thijs
van Grinsven, Bart
Eersels, Kasper
Cornelis, Peter
Kholwadia, Safira
Cleij, Thomas J.
Thoelen, Ronald
De Ceuninck, Ward
Peeters, Marloes
Wagner, Patrick
Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors
title Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors
title_full Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors
title_fullStr Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors
title_full_unstemmed Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors
title_short Single-Shot Detection of Neurotransmitters in Whole-Blood Samples by Means of the Heat-Transfer Method in Combination with Synthetic Receptors
title_sort single-shot detection of neurotransmitters in whole-blood samples by means of the heat-transfer method in combination with synthetic receptors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751640/
https://www.ncbi.nlm.nih.gov/pubmed/29168733
http://dx.doi.org/10.3390/s17122701
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