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DEP1 gene in wheat species with normal, compactoid and compact spikes

BACKGROUND: In rice, a variant of DEP1 gene results in erect panicle architecture, well-developed vascular bundles, an increase in the number of grains per panicle and a consequent increase in the grain yield. Interestingly, DEP1 homologs are present in the other cereals including species of wheat a...

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Autores principales: Vavilova, Valeriya, Konopatskaia, Irina, Kuznetsova, Anastasia E., Blinov, Alexandr, Goncharov, Nikolay P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751790/
https://www.ncbi.nlm.nih.gov/pubmed/29297308
http://dx.doi.org/10.1186/s12863-017-0583-6
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author Vavilova, Valeriya
Konopatskaia, Irina
Kuznetsova, Anastasia E.
Blinov, Alexandr
Goncharov, Nikolay P.
author_facet Vavilova, Valeriya
Konopatskaia, Irina
Kuznetsova, Anastasia E.
Blinov, Alexandr
Goncharov, Nikolay P.
author_sort Vavilova, Valeriya
collection PubMed
description BACKGROUND: In rice, a variant of DEP1 gene results in erect panicle architecture, well-developed vascular bundles, an increase in the number of grains per panicle and a consequent increase in the grain yield. Interestingly, DEP1 homologs are present in the other cereals including species of wheat and barley (Hordeum vulgare), even though they do not produce panicles but spikes. In barley, HvDEP1 alleles do not differ between strains of various ear types and geographic origins, while in at least three OsDEP1 variants have been described. RESULTS: In this work, we have studied the DEP1 gene from eight accessions which belong to four wheat species, T. monococcum, T. durum, T. compactum, and T. spelta, with either compact, compactoid or normal spike phenotypes. The nucleotide sequences of the 5th exon of DEP1 were determined for all eight accessions. Obtained sequences were species specific. Despite the interspecies diversity, all wheat sequences encoded polypeptides of the same size, similarly to the 5th exons of the DEP1 homologs in T. aestivum, T. urartu, and H. vulgare. For further study, the full-length sequences of the DEP1 gene for all four species were studied. The full-length DEP1 genomic copies were isolated from the genomic sequences of T. aestivum, T. urartu, and Aegilops tauschii. The genome of tetraploid wheat T. durum contains two variants of the DEP1 originating from A and B genomes. In the hexaploid wheats T. aestivum, T. compactum, and T. spelta, three variants of this gene originating from A, B, and D genomes were detected. DEP1 genes of the diploid wheats T. monococcum and T. urartu differ. It seems that a precursor of the DEP1 gene in T. monococcum originates from the wild progenitor T. boeoticum. CONCLUSION: No DEP1-related differences of nucleotide sequences between the compact (or compactoid) and normal spike phenotypes in the tested wheat species were detected. Therefore, DEP1 gene does not directly participate in the control of the spike architecture in wheats. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12863-017-0583-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-57517902018-01-05 DEP1 gene in wheat species with normal, compactoid and compact spikes Vavilova, Valeriya Konopatskaia, Irina Kuznetsova, Anastasia E. Blinov, Alexandr Goncharov, Nikolay P. BMC Genet Research BACKGROUND: In rice, a variant of DEP1 gene results in erect panicle architecture, well-developed vascular bundles, an increase in the number of grains per panicle and a consequent increase in the grain yield. Interestingly, DEP1 homologs are present in the other cereals including species of wheat and barley (Hordeum vulgare), even though they do not produce panicles but spikes. In barley, HvDEP1 alleles do not differ between strains of various ear types and geographic origins, while in at least three OsDEP1 variants have been described. RESULTS: In this work, we have studied the DEP1 gene from eight accessions which belong to four wheat species, T. monococcum, T. durum, T. compactum, and T. spelta, with either compact, compactoid or normal spike phenotypes. The nucleotide sequences of the 5th exon of DEP1 were determined for all eight accessions. Obtained sequences were species specific. Despite the interspecies diversity, all wheat sequences encoded polypeptides of the same size, similarly to the 5th exons of the DEP1 homologs in T. aestivum, T. urartu, and H. vulgare. For further study, the full-length sequences of the DEP1 gene for all four species were studied. The full-length DEP1 genomic copies were isolated from the genomic sequences of T. aestivum, T. urartu, and Aegilops tauschii. The genome of tetraploid wheat T. durum contains two variants of the DEP1 originating from A and B genomes. In the hexaploid wheats T. aestivum, T. compactum, and T. spelta, three variants of this gene originating from A, B, and D genomes were detected. DEP1 genes of the diploid wheats T. monococcum and T. urartu differ. It seems that a precursor of the DEP1 gene in T. monococcum originates from the wild progenitor T. boeoticum. CONCLUSION: No DEP1-related differences of nucleotide sequences between the compact (or compactoid) and normal spike phenotypes in the tested wheat species were detected. Therefore, DEP1 gene does not directly participate in the control of the spike architecture in wheats. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12863-017-0583-6) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-28 /pmc/articles/PMC5751790/ /pubmed/29297308 http://dx.doi.org/10.1186/s12863-017-0583-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Vavilova, Valeriya
Konopatskaia, Irina
Kuznetsova, Anastasia E.
Blinov, Alexandr
Goncharov, Nikolay P.
DEP1 gene in wheat species with normal, compactoid and compact spikes
title DEP1 gene in wheat species with normal, compactoid and compact spikes
title_full DEP1 gene in wheat species with normal, compactoid and compact spikes
title_fullStr DEP1 gene in wheat species with normal, compactoid and compact spikes
title_full_unstemmed DEP1 gene in wheat species with normal, compactoid and compact spikes
title_short DEP1 gene in wheat species with normal, compactoid and compact spikes
title_sort dep1 gene in wheat species with normal, compactoid and compact spikes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751790/
https://www.ncbi.nlm.nih.gov/pubmed/29297308
http://dx.doi.org/10.1186/s12863-017-0583-6
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