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Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain

Voltage-gated sodium channels serve an essential role in the initiation and propagation of action potentials for central neurons. Previous studies have demonstrated that two novel variants of Nav1.5, designated Nav1.5e and Nav1.5f, were expressed in the human brain cortex. To date, nine distinct sod...

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Autores principales: Wang, Jun, Ou, Shao-Wu, Zhang, Zhi-Yong, Qiu, Bo, Wang, Yun-Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752160/
https://www.ncbi.nlm.nih.gov/pubmed/29207052
http://dx.doi.org/10.3892/ijmm.2017.3286
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author Wang, Jun
Ou, Shao-Wu
Zhang, Zhi-Yong
Qiu, Bo
Wang, Yun-Jie
author_facet Wang, Jun
Ou, Shao-Wu
Zhang, Zhi-Yong
Qiu, Bo
Wang, Yun-Jie
author_sort Wang, Jun
collection PubMed
description Voltage-gated sodium channels serve an essential role in the initiation and propagation of action potentials for central neurons. Previous studies have demonstrated that two novel variants of Nav1.5, designated Nav1.5e and Nav1.5f, were expressed in the human brain cortex. To date, nine distinct sodium channel isoforms of Nav1.5 have been identified. In the present study, the expression of Nav1.5 splice variants in the frontal lobe of the human brain cortex was systematically investigated. The results demonstrated that wild Nav1.5 and its splice variants, Nav1.5c and Nav1.5e, were expressed in the frontal lobe of the human brain cortex. Nav1.5a, Nav1.5b and Nav1.5d splice variants were not detected. However, the expression level of different Nav1.5 variants was revealed to vary. The expression ratio of wild Nav1.5 vs. Nav1.5c and Nav1.5e was approximately 5:1 and 1:5, respectively. Immunochemistry results revealed that Nav1.5 immunoreactivity was predominantly in neuronal cell bodies and processes, including axons and dendrites, whereas little immunoreactivity was detected in the glial components. These results revealed that a minimum of four Nav1.5 splice variants are expressed in the frontal lobe of the human brain cortex. This indicates that the previously reported tetrodotoxin-resistant sodium current was a compound product of different Nav1.5 variants. The present study revealed that Nav1.5 channels have a more abundant expression in the human brain than previously considered. It also provided further insight into the complexity and functional significance of Nav1.5 channels in human brain neurons.
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spelling pubmed-57521602018-01-11 Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain Wang, Jun Ou, Shao-Wu Zhang, Zhi-Yong Qiu, Bo Wang, Yun-Jie Int J Mol Med Articles Voltage-gated sodium channels serve an essential role in the initiation and propagation of action potentials for central neurons. Previous studies have demonstrated that two novel variants of Nav1.5, designated Nav1.5e and Nav1.5f, were expressed in the human brain cortex. To date, nine distinct sodium channel isoforms of Nav1.5 have been identified. In the present study, the expression of Nav1.5 splice variants in the frontal lobe of the human brain cortex was systematically investigated. The results demonstrated that wild Nav1.5 and its splice variants, Nav1.5c and Nav1.5e, were expressed in the frontal lobe of the human brain cortex. Nav1.5a, Nav1.5b and Nav1.5d splice variants were not detected. However, the expression level of different Nav1.5 variants was revealed to vary. The expression ratio of wild Nav1.5 vs. Nav1.5c and Nav1.5e was approximately 5:1 and 1:5, respectively. Immunochemistry results revealed that Nav1.5 immunoreactivity was predominantly in neuronal cell bodies and processes, including axons and dendrites, whereas little immunoreactivity was detected in the glial components. These results revealed that a minimum of four Nav1.5 splice variants are expressed in the frontal lobe of the human brain cortex. This indicates that the previously reported tetrodotoxin-resistant sodium current was a compound product of different Nav1.5 variants. The present study revealed that Nav1.5 channels have a more abundant expression in the human brain than previously considered. It also provided further insight into the complexity and functional significance of Nav1.5 channels in human brain neurons. D.A. Spandidos 2018-02 2017-11-24 /pmc/articles/PMC5752160/ /pubmed/29207052 http://dx.doi.org/10.3892/ijmm.2017.3286 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Jun
Ou, Shao-Wu
Zhang, Zhi-Yong
Qiu, Bo
Wang, Yun-Jie
Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain
title Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain
title_full Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain
title_fullStr Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain
title_full_unstemmed Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain
title_short Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain
title_sort molecular expression of multiple nav1.5 splice variants in the frontal lobe of the human brain
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752160/
https://www.ncbi.nlm.nih.gov/pubmed/29207052
http://dx.doi.org/10.3892/ijmm.2017.3286
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