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In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats
Postmenopausal osteoporosis (PMO) increases bone fragility and the risk of fractures, and impairs the healing procedure of bone defects in aged women. The stromal cell-derived factor-1α (SDF-1α)/CXC chemokine receptor type 4 (CXCR4) axis helps to maintain the biological and physiological functions o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752170/ https://www.ncbi.nlm.nih.gov/pubmed/29207050 http://dx.doi.org/10.3892/ijmm.2017.3280 |
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author | Liu, Qian Zhang, Xiaoxia Jiao, Yang Liu, Xin Wang, Yirong Li, Song-Lun Zhang, Wei Chen, Fa-Ming Ding, Yin Jiang, Chuan Jin, Zuolin |
author_facet | Liu, Qian Zhang, Xiaoxia Jiao, Yang Liu, Xin Wang, Yirong Li, Song-Lun Zhang, Wei Chen, Fa-Ming Ding, Yin Jiang, Chuan Jin, Zuolin |
author_sort | Liu, Qian |
collection | PubMed |
description | Postmenopausal osteoporosis (PMO) increases bone fragility and the risk of fractures, and impairs the healing procedure of bone defects in aged women. The stromal cell-derived factor-1α (SDF-1α)/CXC chemokine receptor type 4 (CXCR4) axis helps to maintain the biological and physiological functions of bone marrow mesenchymal stem cells (BMSCs) and increase the homing efficiency of BMSCs. The present study aimed to provide insights into the possible association between migration and osteogenic ability and the SDF-1α/CXCR4 axis in BMSCs derived from a rat model of PMO. In order to do this, the general and SDF-1α/CXCR4-associated biological characteristics as well as associated molecular mechanisms in BMSCs isolated from a PMO rat model (OVX-BMSCs) and normal rats (Sham-BMSCs) were investigated and compared. In comparison with Sham-BMSCs, OVX-BMSCs exhibited an impaired osteogenic ability, but a stronger adipogenic activity as well as a higher proliferative ability. In addition, OVX-BMSCs presented a lower chemotactic activity towards SDF-1α, lower expression levels of CXCR4 and reduced levels of phosphorylated AKT (p-AKT). Therefore, the lower expression levels of CXCR4 and p-AKT may be responsible for the impaired osteogenic ability and lower chemotactic activity towards SDF-1α of OVX-BMSCs. |
format | Online Article Text |
id | pubmed-5752170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57521702018-01-11 In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats Liu, Qian Zhang, Xiaoxia Jiao, Yang Liu, Xin Wang, Yirong Li, Song-Lun Zhang, Wei Chen, Fa-Ming Ding, Yin Jiang, Chuan Jin, Zuolin Int J Mol Med Articles Postmenopausal osteoporosis (PMO) increases bone fragility and the risk of fractures, and impairs the healing procedure of bone defects in aged women. The stromal cell-derived factor-1α (SDF-1α)/CXC chemokine receptor type 4 (CXCR4) axis helps to maintain the biological and physiological functions of bone marrow mesenchymal stem cells (BMSCs) and increase the homing efficiency of BMSCs. The present study aimed to provide insights into the possible association between migration and osteogenic ability and the SDF-1α/CXCR4 axis in BMSCs derived from a rat model of PMO. In order to do this, the general and SDF-1α/CXCR4-associated biological characteristics as well as associated molecular mechanisms in BMSCs isolated from a PMO rat model (OVX-BMSCs) and normal rats (Sham-BMSCs) were investigated and compared. In comparison with Sham-BMSCs, OVX-BMSCs exhibited an impaired osteogenic ability, but a stronger adipogenic activity as well as a higher proliferative ability. In addition, OVX-BMSCs presented a lower chemotactic activity towards SDF-1α, lower expression levels of CXCR4 and reduced levels of phosphorylated AKT (p-AKT). Therefore, the lower expression levels of CXCR4 and p-AKT may be responsible for the impaired osteogenic ability and lower chemotactic activity towards SDF-1α of OVX-BMSCs. D.A. Spandidos 2018-02 2017-11-22 /pmc/articles/PMC5752170/ /pubmed/29207050 http://dx.doi.org/10.3892/ijmm.2017.3280 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Qian Zhang, Xiaoxia Jiao, Yang Liu, Xin Wang, Yirong Li, Song-Lun Zhang, Wei Chen, Fa-Ming Ding, Yin Jiang, Chuan Jin, Zuolin In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
title | In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
title_full | In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
title_fullStr | In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
title_full_unstemmed | In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
title_short | In vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
title_sort | in vitro cell behaviors of bone mesenchymal stem cells derived from normal and postmenopausal osteoporotic rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752170/ https://www.ncbi.nlm.nih.gov/pubmed/29207050 http://dx.doi.org/10.3892/ijmm.2017.3280 |
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