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Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling
We investigated the mechanisms that induce atrophy of the chicken bursa of Fabricius (BF) upon lipopolysaccharide (LPS) treatment in young chicks. LPS treatment resulted in ∼36% decrease in bursal weight within 36 h (P < 0.01). Histological analysis showed infiltration of eosinophilic heterophils...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752450/ https://www.ncbi.nlm.nih.gov/pubmed/29312537 http://dx.doi.org/10.18632/oncotarget.19964 |
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author | Ansari, Abdur Rahman Li, Ning-Ya Sun, Zhi-Jian Huang, Hai-Bo Zhao, Xing Cui, Lei Hu, Ya-Fang Zhong, Ju-Ming Karrow, Niel A. Liu, Hua-Zhen |
author_facet | Ansari, Abdur Rahman Li, Ning-Ya Sun, Zhi-Jian Huang, Hai-Bo Zhao, Xing Cui, Lei Hu, Ya-Fang Zhong, Ju-Ming Karrow, Niel A. Liu, Hua-Zhen |
author_sort | Ansari, Abdur Rahman |
collection | PubMed |
description | We investigated the mechanisms that induce atrophy of the chicken bursa of Fabricius (BF) upon lipopolysaccharide (LPS) treatment in young chicks. LPS treatment resulted in ∼36% decrease in bursal weight within 36 h (P < 0.01). Histological analysis showed infiltration of eosinophilic heterophils and nucleated oval shaped RBCs in or near blood vessels of the BF from LPS-treated chicks. Scanning electron micrographs showed severe erosion and breaks in the mucosal membrane at 12 h and complete exuviation of bursal mucosal epithelial cells at 36 h. We observed decreased cell proliferation (low PCNA positivity) and increased apoptosis (high TUNEL and ssDNA positivity) in the BF 12-72 h after LPS treatment. RNA-seq analysis of the BF transcriptome showed 736 differentially expressed genes with most expression changes (637/736) 12 h after LPS treatment. KEGG pathway analysis identified TLR4-MAPK-NF-κB/AP-1 as the key signaling pathway affected in response to LPS stimulation. These findings indicate LPS activates the TLR4-MAPK-NF-κB/AP-1 signaling pathway that mediates acute atrophy of the chicken bursa of Fabricius by inducing inflammation and apoptosis. |
format | Online Article Text |
id | pubmed-5752450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57524502018-01-08 Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling Ansari, Abdur Rahman Li, Ning-Ya Sun, Zhi-Jian Huang, Hai-Bo Zhao, Xing Cui, Lei Hu, Ya-Fang Zhong, Ju-Ming Karrow, Niel A. Liu, Hua-Zhen Oncotarget Research Paper: Immunology We investigated the mechanisms that induce atrophy of the chicken bursa of Fabricius (BF) upon lipopolysaccharide (LPS) treatment in young chicks. LPS treatment resulted in ∼36% decrease in bursal weight within 36 h (P < 0.01). Histological analysis showed infiltration of eosinophilic heterophils and nucleated oval shaped RBCs in or near blood vessels of the BF from LPS-treated chicks. Scanning electron micrographs showed severe erosion and breaks in the mucosal membrane at 12 h and complete exuviation of bursal mucosal epithelial cells at 36 h. We observed decreased cell proliferation (low PCNA positivity) and increased apoptosis (high TUNEL and ssDNA positivity) in the BF 12-72 h after LPS treatment. RNA-seq analysis of the BF transcriptome showed 736 differentially expressed genes with most expression changes (637/736) 12 h after LPS treatment. KEGG pathway analysis identified TLR4-MAPK-NF-κB/AP-1 as the key signaling pathway affected in response to LPS stimulation. These findings indicate LPS activates the TLR4-MAPK-NF-κB/AP-1 signaling pathway that mediates acute atrophy of the chicken bursa of Fabricius by inducing inflammation and apoptosis. Impact Journals LLC 2017-08-05 /pmc/articles/PMC5752450/ /pubmed/29312537 http://dx.doi.org/10.18632/oncotarget.19964 Text en Copyright: © 2017 Ansari et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Immunology Ansari, Abdur Rahman Li, Ning-Ya Sun, Zhi-Jian Huang, Hai-Bo Zhao, Xing Cui, Lei Hu, Ya-Fang Zhong, Ju-Ming Karrow, Niel A. Liu, Hua-Zhen Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling |
title | Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling |
title_full | Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling |
title_fullStr | Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling |
title_full_unstemmed | Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling |
title_short | Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling |
title_sort | lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating tlr4-mapk-nf-κb/ap-1 signaling |
topic | Research Paper: Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752450/ https://www.ncbi.nlm.nih.gov/pubmed/29312537 http://dx.doi.org/10.18632/oncotarget.19964 |
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