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Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells

Hepatocellular carcinoma (HCC) remains the third cause of cancer-related mortality. Resection and transplantation are the only curative treatments available but are greatly hampered by high recurrence rates and development of metastasis, the initiation of cancer metastasis requires migration and inv...

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Autores principales: Cao, Meng-Ting, Liu, Hui-Fang, Liu, Zhi-Gang, Xiao, Ping, Chen, Jing-Jing, Tan, Yuan, Jiang, Xiao-Xin, Jiang, Zhi-Chao, Qiu, Yu, Huang, Hong-Jun, Zhang, Qiu-Gui, Jiang, Guan-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752459/
https://www.ncbi.nlm.nih.gov/pubmed/29312546
http://dx.doi.org/10.18632/oncotarget.22590
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author Cao, Meng-Ting
Liu, Hui-Fang
Liu, Zhi-Gang
Xiao, Ping
Chen, Jing-Jing
Tan, Yuan
Jiang, Xiao-Xin
Jiang, Zhi-Chao
Qiu, Yu
Huang, Hong-Jun
Zhang, Qiu-Gui
Jiang, Guan-Min
author_facet Cao, Meng-Ting
Liu, Hui-Fang
Liu, Zhi-Gang
Xiao, Ping
Chen, Jing-Jing
Tan, Yuan
Jiang, Xiao-Xin
Jiang, Zhi-Chao
Qiu, Yu
Huang, Hong-Jun
Zhang, Qiu-Gui
Jiang, Guan-Min
author_sort Cao, Meng-Ting
collection PubMed
description Hepatocellular carcinoma (HCC) remains the third cause of cancer-related mortality. Resection and transplantation are the only curative treatments available but are greatly hampered by high recurrence rates and development of metastasis, the initiation of cancer metastasis requires migration and invasion of cells, which is enabled by epithelial-mesenchymal transitions (EMT). TGF-β1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGF-β1 is known as a major inducer of EMT, and it was reported that TGF-β1 induced EMT via Smad-dependent and Smad-independent pathways. However, the extrinsic signals of TGF-β1 regulated the EMT in hepatoma cells remains to be elucidated, and searching drugs to inhibit TGF-β1 induced EMT may be considered to be a potentially effective therapeutic strategy in HCC. Fortunately, in this study, we found that curcumin inhibited TGF-β1-induced EMT in hepatoma cells. Furthermore, we demonstrated that curcumin inhibited TGF-β1-induced EMT via inhibiting Smad2 phosphorylation and nuclear translocation, then suppressing Smad2 combined with the promoter of Snail which inhibited the transcriptional expression of Snail. These findings suggesting curcumin could be a useful agent for antitumor therapy and also a promising drug combined with other strategies to preventing and treating HCC.
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spelling pubmed-57524592018-01-08 Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells Cao, Meng-Ting Liu, Hui-Fang Liu, Zhi-Gang Xiao, Ping Chen, Jing-Jing Tan, Yuan Jiang, Xiao-Xin Jiang, Zhi-Chao Qiu, Yu Huang, Hong-Jun Zhang, Qiu-Gui Jiang, Guan-Min Oncotarget Research Paper Hepatocellular carcinoma (HCC) remains the third cause of cancer-related mortality. Resection and transplantation are the only curative treatments available but are greatly hampered by high recurrence rates and development of metastasis, the initiation of cancer metastasis requires migration and invasion of cells, which is enabled by epithelial-mesenchymal transitions (EMT). TGF-β1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGF-β1 is known as a major inducer of EMT, and it was reported that TGF-β1 induced EMT via Smad-dependent and Smad-independent pathways. However, the extrinsic signals of TGF-β1 regulated the EMT in hepatoma cells remains to be elucidated, and searching drugs to inhibit TGF-β1 induced EMT may be considered to be a potentially effective therapeutic strategy in HCC. Fortunately, in this study, we found that curcumin inhibited TGF-β1-induced EMT in hepatoma cells. Furthermore, we demonstrated that curcumin inhibited TGF-β1-induced EMT via inhibiting Smad2 phosphorylation and nuclear translocation, then suppressing Smad2 combined with the promoter of Snail which inhibited the transcriptional expression of Snail. These findings suggesting curcumin could be a useful agent for antitumor therapy and also a promising drug combined with other strategies to preventing and treating HCC. Impact Journals LLC 2017-11-21 /pmc/articles/PMC5752459/ /pubmed/29312546 http://dx.doi.org/10.18632/oncotarget.22590 Text en Copyright: © 2017 Cao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Cao, Meng-Ting
Liu, Hui-Fang
Liu, Zhi-Gang
Xiao, Ping
Chen, Jing-Jing
Tan, Yuan
Jiang, Xiao-Xin
Jiang, Zhi-Chao
Qiu, Yu
Huang, Hong-Jun
Zhang, Qiu-Gui
Jiang, Guan-Min
Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells
title Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells
title_full Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells
title_fullStr Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells
title_full_unstemmed Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells
title_short Curcumin downregulates the expression of Snail via suppressing Smad2 pathway to inhibit TGF-β1-induced epithelial-mesenchymal transitions in hepatoma cells
title_sort curcumin downregulates the expression of snail via suppressing smad2 pathway to inhibit tgf-β1-induced epithelial-mesenchymal transitions in hepatoma cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752459/
https://www.ncbi.nlm.nih.gov/pubmed/29312546
http://dx.doi.org/10.18632/oncotarget.22590
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