Cargando…
Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish
Mild traumatic brain injuries (mTBIs) are one of the most prevalent neurological disorders, and humans are severely limited in their ability to repair and regenerate central nervous system (CNS) tissue postinjury. However, zebrafish (Danio rerio) maintain the remarkable ability to undergo complete a...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752677/ https://www.ncbi.nlm.nih.gov/pubmed/29302617 http://dx.doi.org/10.1523/ENEURO.0208-17.2017 |
_version_ | 1783290144433897472 |
---|---|
author | Maheras, Amanda L. Dix, Brian Carmo, Olivia M. S. Young, Aleena E. Gill, Vanessa N. Sun, Julia L. Booker, Aleah R. Thomason, Helen A. Ibrahim, Anastasia E. Stanislaw, Lauren Dallego, Jennifer C. Ngo, Cat N. Chen, Audrey Fortini, Barbara K. Spence, Rory D. |
author_facet | Maheras, Amanda L. Dix, Brian Carmo, Olivia M. S. Young, Aleena E. Gill, Vanessa N. Sun, Julia L. Booker, Aleah R. Thomason, Helen A. Ibrahim, Anastasia E. Stanislaw, Lauren Dallego, Jennifer C. Ngo, Cat N. Chen, Audrey Fortini, Barbara K. Spence, Rory D. |
author_sort | Maheras, Amanda L. |
collection | PubMed |
description | Mild traumatic brain injuries (mTBIs) are one of the most prevalent neurological disorders, and humans are severely limited in their ability to repair and regenerate central nervous system (CNS) tissue postinjury. However, zebrafish (Danio rerio) maintain the remarkable ability to undergo complete and functional neuroregeneration as an adult. We wish to extend knowledge of the known mechanisms of neuroregeneration by analyzing the differentially expressed genes (DEGs) in a novel adult zebrafish model of mTBI. In this study, a rodent weight drop model of mTBI was adapted to the adult zebrafish. A memory test showed significant deficits in spatial memory in the mTBI group. We identified DEGs at 3 and 21 days postinjury (dpi) through RNA-sequencing analysis. The resulting DEGs were categorized according to gene ontology (GO) categories. At 3 dpi, GO categories consisted of peak injury response pathways. Significantly, at 21 dpi, GO categories consisted of neuroregeneration pathways. Ultimately, these results validate a novel zebrafish model of mTBI and elucidate significant DEGs of interest in CNS injury and neuroregeneration. |
format | Online Article Text |
id | pubmed-5752677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-57526772018-01-04 Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish Maheras, Amanda L. Dix, Brian Carmo, Olivia M. S. Young, Aleena E. Gill, Vanessa N. Sun, Julia L. Booker, Aleah R. Thomason, Helen A. Ibrahim, Anastasia E. Stanislaw, Lauren Dallego, Jennifer C. Ngo, Cat N. Chen, Audrey Fortini, Barbara K. Spence, Rory D. eNeuro New Research Mild traumatic brain injuries (mTBIs) are one of the most prevalent neurological disorders, and humans are severely limited in their ability to repair and regenerate central nervous system (CNS) tissue postinjury. However, zebrafish (Danio rerio) maintain the remarkable ability to undergo complete and functional neuroregeneration as an adult. We wish to extend knowledge of the known mechanisms of neuroregeneration by analyzing the differentially expressed genes (DEGs) in a novel adult zebrafish model of mTBI. In this study, a rodent weight drop model of mTBI was adapted to the adult zebrafish. A memory test showed significant deficits in spatial memory in the mTBI group. We identified DEGs at 3 and 21 days postinjury (dpi) through RNA-sequencing analysis. The resulting DEGs were categorized according to gene ontology (GO) categories. At 3 dpi, GO categories consisted of peak injury response pathways. Significantly, at 21 dpi, GO categories consisted of neuroregeneration pathways. Ultimately, these results validate a novel zebrafish model of mTBI and elucidate significant DEGs of interest in CNS injury and neuroregeneration. Society for Neuroscience 2018-01-03 /pmc/articles/PMC5752677/ /pubmed/29302617 http://dx.doi.org/10.1523/ENEURO.0208-17.2017 Text en Copyright © 2018 Maheras et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | New Research Maheras, Amanda L. Dix, Brian Carmo, Olivia M. S. Young, Aleena E. Gill, Vanessa N. Sun, Julia L. Booker, Aleah R. Thomason, Helen A. Ibrahim, Anastasia E. Stanislaw, Lauren Dallego, Jennifer C. Ngo, Cat N. Chen, Audrey Fortini, Barbara K. Spence, Rory D. Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish |
title | Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish |
title_full | Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish |
title_fullStr | Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish |
title_full_unstemmed | Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish |
title_short | Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish |
title_sort | genetic pathways of neuroregeneration in a novel mild traumatic brain injury model in adult zebrafish |
topic | New Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752677/ https://www.ncbi.nlm.nih.gov/pubmed/29302617 http://dx.doi.org/10.1523/ENEURO.0208-17.2017 |
work_keys_str_mv | AT maherasamandal geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT dixbrian geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT carmooliviams geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT youngaleenae geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT gillvanessan geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT sunjulial geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT bookeraleahr geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT thomasonhelena geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT ibrahimanastasiae geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT stanislawlauren geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT dallegojenniferc geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT ngocatn geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT chenaudrey geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT fortinibarbarak geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish AT spenceroryd geneticpathwaysofneuroregenerationinanovelmildtraumaticbraininjurymodelinadultzebrafish |