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Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights

AIM: To provide new insights in treatment of colitis and ischemia and reperfusion in rats using stable gastric pentadecapeptide BPC 157. METHODS: Medication [BPC 157, L-NAME, L-arginine (alone/combined), saline] was bath at the blood deprived colon segment. During reperfusion, medication was BPC 157...

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Autores principales: Duzel, Antonija, Vlainic, Josipa, Antunovic, Marko, Malekinusic, Dominik, Vrdoljak, Borna, Samara, Mariam, Gojkovic, Slaven, Krezic, Ivan, Vidovic, Tinka, Bilic, Zdenko, Knezevic, Mario, Sever, Marko, Lojo, Nermin, Kokot, Antonio, Kolovrat, Marijan, Drmic, Domagoj, Vukojevic, Jaksa, Kralj, Tamara, Kasnik, Katarina, Siroglavic, Marko, Seiwerth, Sven, Sikiric, Predrag
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752708/
https://www.ncbi.nlm.nih.gov/pubmed/29358856
http://dx.doi.org/10.3748/wjg.v23.i48.8465
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author Duzel, Antonija
Vlainic, Josipa
Antunovic, Marko
Malekinusic, Dominik
Vrdoljak, Borna
Samara, Mariam
Gojkovic, Slaven
Krezic, Ivan
Vidovic, Tinka
Bilic, Zdenko
Knezevic, Mario
Sever, Marko
Lojo, Nermin
Kokot, Antonio
Kolovrat, Marijan
Drmic, Domagoj
Vukojevic, Jaksa
Kralj, Tamara
Kasnik, Katarina
Siroglavic, Marko
Seiwerth, Sven
Sikiric, Predrag
author_facet Duzel, Antonija
Vlainic, Josipa
Antunovic, Marko
Malekinusic, Dominik
Vrdoljak, Borna
Samara, Mariam
Gojkovic, Slaven
Krezic, Ivan
Vidovic, Tinka
Bilic, Zdenko
Knezevic, Mario
Sever, Marko
Lojo, Nermin
Kokot, Antonio
Kolovrat, Marijan
Drmic, Domagoj
Vukojevic, Jaksa
Kralj, Tamara
Kasnik, Katarina
Siroglavic, Marko
Seiwerth, Sven
Sikiric, Predrag
author_sort Duzel, Antonija
collection PubMed
description AIM: To provide new insights in treatment of colitis and ischemia and reperfusion in rats using stable gastric pentadecapeptide BPC 157. METHODS: Medication [BPC 157, L-NAME, L-arginine (alone/combined), saline] was bath at the blood deprived colon segment. During reperfusion, medication was BPC 157 or saline. We recorded (USB microscope camera) vessel presentation through next 15 min of ischemic colitis (IC-rats) or reperfusion (removed ligations) (IC + RL-rats); oxidative stress as MDA (increased (IC- and IC + RL-rats)) and NO levels (decreased (IC-rats); increased (IC + RL-rats)) in colon tissue. IC + OB-rats [IC-rats had additional colon obstruction (OB)] for 3 d (IC + OB-rats), then received BPC 157 bath. RESULTS: Commonly, in colon segment (25 mm, 2 ligations on left colic artery and vein, 3 arcade vessels within ligated segment), in IC-, IC + RL-, IC + OB-rats, BPC 157 (10 μg/kg) bath (1 mL/rat) increased vessel presentation, inside/outside arcade interconnections quickly reappeared, mucosal folds were preserved and the pale areas were small and markedly reduced. BPC 157 counteracted worsening effects induced by L-NAME (5 mg) and L-arginine (100 mg). MDA- and NO-levels were normal in BPC 157 treated IC-rats and IC + RL-rats. In addition, on day 10, BPC 157-treated IC + OB-rats presented almost completely spared mucosa with very small pale areas and no gross mucosal defects; the treated colon segment was of normal diameter, and only small adhesions were present. CONCLUSION: BPC 157 is a fundamental treatment that quickly restores blood supply to the ischemically injured area and rapidly activates collaterals. This effect involves the NO system.
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spelling pubmed-57527082018-01-22 Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights Duzel, Antonija Vlainic, Josipa Antunovic, Marko Malekinusic, Dominik Vrdoljak, Borna Samara, Mariam Gojkovic, Slaven Krezic, Ivan Vidovic, Tinka Bilic, Zdenko Knezevic, Mario Sever, Marko Lojo, Nermin Kokot, Antonio Kolovrat, Marijan Drmic, Domagoj Vukojevic, Jaksa Kralj, Tamara Kasnik, Katarina Siroglavic, Marko Seiwerth, Sven Sikiric, Predrag World J Gastroenterol Basic Study AIM: To provide new insights in treatment of colitis and ischemia and reperfusion in rats using stable gastric pentadecapeptide BPC 157. METHODS: Medication [BPC 157, L-NAME, L-arginine (alone/combined), saline] was bath at the blood deprived colon segment. During reperfusion, medication was BPC 157 or saline. We recorded (USB microscope camera) vessel presentation through next 15 min of ischemic colitis (IC-rats) or reperfusion (removed ligations) (IC + RL-rats); oxidative stress as MDA (increased (IC- and IC + RL-rats)) and NO levels (decreased (IC-rats); increased (IC + RL-rats)) in colon tissue. IC + OB-rats [IC-rats had additional colon obstruction (OB)] for 3 d (IC + OB-rats), then received BPC 157 bath. RESULTS: Commonly, in colon segment (25 mm, 2 ligations on left colic artery and vein, 3 arcade vessels within ligated segment), in IC-, IC + RL-, IC + OB-rats, BPC 157 (10 μg/kg) bath (1 mL/rat) increased vessel presentation, inside/outside arcade interconnections quickly reappeared, mucosal folds were preserved and the pale areas were small and markedly reduced. BPC 157 counteracted worsening effects induced by L-NAME (5 mg) and L-arginine (100 mg). MDA- and NO-levels were normal in BPC 157 treated IC-rats and IC + RL-rats. In addition, on day 10, BPC 157-treated IC + OB-rats presented almost completely spared mucosa with very small pale areas and no gross mucosal defects; the treated colon segment was of normal diameter, and only small adhesions were present. CONCLUSION: BPC 157 is a fundamental treatment that quickly restores blood supply to the ischemically injured area and rapidly activates collaterals. This effect involves the NO system. Baishideng Publishing Group Inc 2017-12-28 2017-12-28 /pmc/articles/PMC5752708/ /pubmed/29358856 http://dx.doi.org/10.3748/wjg.v23.i48.8465 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Duzel, Antonija
Vlainic, Josipa
Antunovic, Marko
Malekinusic, Dominik
Vrdoljak, Borna
Samara, Mariam
Gojkovic, Slaven
Krezic, Ivan
Vidovic, Tinka
Bilic, Zdenko
Knezevic, Mario
Sever, Marko
Lojo, Nermin
Kokot, Antonio
Kolovrat, Marijan
Drmic, Domagoj
Vukojevic, Jaksa
Kralj, Tamara
Kasnik, Katarina
Siroglavic, Marko
Seiwerth, Sven
Sikiric, Predrag
Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
title Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
title_full Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
title_fullStr Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
title_full_unstemmed Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
title_short Stable gastric pentadecapeptide BPC 157 in the treatment of colitis and ischemia and reperfusion in rats: New insights
title_sort stable gastric pentadecapeptide bpc 157 in the treatment of colitis and ischemia and reperfusion in rats: new insights
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752708/
https://www.ncbi.nlm.nih.gov/pubmed/29358856
http://dx.doi.org/10.3748/wjg.v23.i48.8465
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