Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy

Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA. Despite being...

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Detalles Bibliográficos
Autores principales: Richter, Katharina N, Revelo, Natalia H, Seitz, Katharina J, Helm, Martin S, Sarkar, Deblina, Saleeb, Rebecca S, D'Este, Elisa, Eberle, Jessica, Wagner, Eva, Vogl, Christian, Lazaro, Diana F, Richter, Frank, Coy‐Vergara, Javier, Coceano, Giovanna, Boyden, Edward S, Duncan, Rory R, Hell, Stefan W, Lauterbach, Marcel A, Lehnart, Stephan E, Moser, Tobias, Outeiro, Tiago F, Rehling, Peter, Schwappach, Blanche, Testa, Ilaria, Zapiec, Bolek, Rizzoli, Silvio O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753035/
https://www.ncbi.nlm.nih.gov/pubmed/29146773
http://dx.doi.org/10.15252/embj.201695709

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