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Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase
Parkinson's disease predisposing LRRK2 kinase phosphorylates a group of Rab GTPase proteins including Rab29, within the effector‐binding switch II motif. Previous work indicated that Rab29, located within the PARK16 locus mutated in Parkinson's patients, operates in a common pathway with L...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753036/ https://www.ncbi.nlm.nih.gov/pubmed/29212815 http://dx.doi.org/10.15252/embj.201798099 |
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author | Purlyte, Elena Dhekne, Herschel S Sarhan, Adil R Gomez, Rachel Lis, Pawel Wightman, Melanie Martinez, Terina N Tonelli, Francesca Pfeffer, Suzanne R Alessi, Dario R |
author_facet | Purlyte, Elena Dhekne, Herschel S Sarhan, Adil R Gomez, Rachel Lis, Pawel Wightman, Melanie Martinez, Terina N Tonelli, Francesca Pfeffer, Suzanne R Alessi, Dario R |
author_sort | Purlyte, Elena |
collection | PubMed |
description | Parkinson's disease predisposing LRRK2 kinase phosphorylates a group of Rab GTPase proteins including Rab29, within the effector‐binding switch II motif. Previous work indicated that Rab29, located within the PARK16 locus mutated in Parkinson's patients, operates in a common pathway with LRRK2. Here, we show that Rab29 recruits LRRK2 to the trans‐Golgi network and greatly stimulates its kinase activity. Pathogenic LRRK2 R1441G/C and Y1699C mutants that promote GTP binding are more readily recruited to the Golgi and activated by Rab29 than wild‐type LRRK2. We identify conserved residues within the LRRK2 ankyrin domain that are required for Rab29‐mediated Golgi recruitment and kinase activation. Consistent with these findings, knockout of Rab29 in A549 cells reduces endogenous LRRK2‐mediated phosphorylation of Rab10. We show that mutations that prevent LRRK2 from interacting with either Rab29 or GTP strikingly inhibit phosphorylation of a cluster of highly studied biomarker phosphorylation sites (Ser910, Ser935, Ser955 and Ser973). Our data reveal that Rab29 is a master regulator of LRRK2, controlling its activation, localization, and potentially biomarker phosphorylation. |
format | Online Article Text |
id | pubmed-5753036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57530362018-01-05 Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase Purlyte, Elena Dhekne, Herschel S Sarhan, Adil R Gomez, Rachel Lis, Pawel Wightman, Melanie Martinez, Terina N Tonelli, Francesca Pfeffer, Suzanne R Alessi, Dario R EMBO J Articles Parkinson's disease predisposing LRRK2 kinase phosphorylates a group of Rab GTPase proteins including Rab29, within the effector‐binding switch II motif. Previous work indicated that Rab29, located within the PARK16 locus mutated in Parkinson's patients, operates in a common pathway with LRRK2. Here, we show that Rab29 recruits LRRK2 to the trans‐Golgi network and greatly stimulates its kinase activity. Pathogenic LRRK2 R1441G/C and Y1699C mutants that promote GTP binding are more readily recruited to the Golgi and activated by Rab29 than wild‐type LRRK2. We identify conserved residues within the LRRK2 ankyrin domain that are required for Rab29‐mediated Golgi recruitment and kinase activation. Consistent with these findings, knockout of Rab29 in A549 cells reduces endogenous LRRK2‐mediated phosphorylation of Rab10. We show that mutations that prevent LRRK2 from interacting with either Rab29 or GTP strikingly inhibit phosphorylation of a cluster of highly studied biomarker phosphorylation sites (Ser910, Ser935, Ser955 and Ser973). Our data reveal that Rab29 is a master regulator of LRRK2, controlling its activation, localization, and potentially biomarker phosphorylation. John Wiley and Sons Inc. 2017-12-06 2018-01-04 /pmc/articles/PMC5753036/ /pubmed/29212815 http://dx.doi.org/10.15252/embj.201798099 Text en © 2017 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution 4.0 (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Purlyte, Elena Dhekne, Herschel S Sarhan, Adil R Gomez, Rachel Lis, Pawel Wightman, Melanie Martinez, Terina N Tonelli, Francesca Pfeffer, Suzanne R Alessi, Dario R Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase |
title | Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase |
title_full | Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase |
title_fullStr | Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase |
title_full_unstemmed | Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase |
title_short | Rab29 activation of the Parkinson's disease‐associated LRRK2 kinase |
title_sort | rab29 activation of the parkinson's disease‐associated lrrk2 kinase |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753036/ https://www.ncbi.nlm.nih.gov/pubmed/29212815 http://dx.doi.org/10.15252/embj.201798099 |
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