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Omics for Investigating Chitosan as an Antifungal and Gene Modulator

Chitosan is a biopolymer with a wide range of applications. The use of chitosan in clinical medicine to control infections by fungal pathogens such as Candida spp. is one of its most promising applications in view of the reduced number of antifungals available. Chitosan increases intracellular oxida...

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Autores principales: Lopez-Moya, Federico, Lopez-Llorca, Luis V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753092/
https://www.ncbi.nlm.nih.gov/pubmed/29376928
http://dx.doi.org/10.3390/jof2010011
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author Lopez-Moya, Federico
Lopez-Llorca, Luis V.
author_facet Lopez-Moya, Federico
Lopez-Llorca, Luis V.
author_sort Lopez-Moya, Federico
collection PubMed
description Chitosan is a biopolymer with a wide range of applications. The use of chitosan in clinical medicine to control infections by fungal pathogens such as Candida spp. is one of its most promising applications in view of the reduced number of antifungals available. Chitosan increases intracellular oxidative stress, then permeabilizes the plasma membrane of sensitive filamentous fungus Neurospora crassa and yeast. Transcriptomics reveals plasma membrane homeostasis and oxidative metabolism genes as key players in the response of fungi to chitosan. A lipase and a monosaccharide transporter, both inner plasma membrane proteins, and a glutathione transferase are main chitosan targets in N. crassa. Biocontrol fungi such as Pochonia chlamydosporia have a low content of polyunsaturated free fatty acids in their plasma membranes and are resistant to chitosan. Genome sequencing of P. chlamydosporia reveals a wide gene machinery to degrade and assimilate chitosan. Chitosan increases P. chlamydosporia sporulation and enhances parasitism of plant parasitic nematodes by the fungus. Omics studies allow understanding the mode of action of chitosan and help its development as an antifungal and gene modulator.
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spelling pubmed-57530922018-01-19 Omics for Investigating Chitosan as an Antifungal and Gene Modulator Lopez-Moya, Federico Lopez-Llorca, Luis V. J Fungi (Basel) Review Chitosan is a biopolymer with a wide range of applications. The use of chitosan in clinical medicine to control infections by fungal pathogens such as Candida spp. is one of its most promising applications in view of the reduced number of antifungals available. Chitosan increases intracellular oxidative stress, then permeabilizes the plasma membrane of sensitive filamentous fungus Neurospora crassa and yeast. Transcriptomics reveals plasma membrane homeostasis and oxidative metabolism genes as key players in the response of fungi to chitosan. A lipase and a monosaccharide transporter, both inner plasma membrane proteins, and a glutathione transferase are main chitosan targets in N. crassa. Biocontrol fungi such as Pochonia chlamydosporia have a low content of polyunsaturated free fatty acids in their plasma membranes and are resistant to chitosan. Genome sequencing of P. chlamydosporia reveals a wide gene machinery to degrade and assimilate chitosan. Chitosan increases P. chlamydosporia sporulation and enhances parasitism of plant parasitic nematodes by the fungus. Omics studies allow understanding the mode of action of chitosan and help its development as an antifungal and gene modulator. MDPI 2016-03-03 /pmc/articles/PMC5753092/ /pubmed/29376928 http://dx.doi.org/10.3390/jof2010011 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lopez-Moya, Federico
Lopez-Llorca, Luis V.
Omics for Investigating Chitosan as an Antifungal and Gene Modulator
title Omics for Investigating Chitosan as an Antifungal and Gene Modulator
title_full Omics for Investigating Chitosan as an Antifungal and Gene Modulator
title_fullStr Omics for Investigating Chitosan as an Antifungal and Gene Modulator
title_full_unstemmed Omics for Investigating Chitosan as an Antifungal and Gene Modulator
title_short Omics for Investigating Chitosan as an Antifungal and Gene Modulator
title_sort omics for investigating chitosan as an antifungal and gene modulator
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753092/
https://www.ncbi.nlm.nih.gov/pubmed/29376928
http://dx.doi.org/10.3390/jof2010011
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