Synthetic mRNA capping

Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. W...

Descripción completa

Detalles Bibliográficos
Autores principales: Muttach, Fabian, Muthmann, Nils, Rentmeister, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2017
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753152/
https://www.ncbi.nlm.nih.gov/pubmed/30018667
http://dx.doi.org/10.3762/bjoc.13.274
_version_ 1783290216896790528
author Muttach, Fabian
Muthmann, Nils
Rentmeister, Andrea
author_facet Muttach, Fabian
Muthmann, Nils
Rentmeister, Andrea
author_sort Muttach, Fabian
collection PubMed
description Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency.
format Online
Article
Text
id pubmed-5753152
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Beilstein-Institut
record_format MEDLINE/PubMed
spelling pubmed-57531522018-07-17 Synthetic mRNA capping Muttach, Fabian Muthmann, Nils Rentmeister, Andrea Beilstein J Org Chem Review Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency. Beilstein-Institut 2017-12-20 /pmc/articles/PMC5753152/ /pubmed/30018667 http://dx.doi.org/10.3762/bjoc.13.274 Text en Copyright © 2017, Muttach et al. https://creativecommons.org/licenses/by/4.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Review
Muttach, Fabian
Muthmann, Nils
Rentmeister, Andrea
Synthetic mRNA capping
title Synthetic mRNA capping
title_full Synthetic mRNA capping
title_fullStr Synthetic mRNA capping
title_full_unstemmed Synthetic mRNA capping
title_short Synthetic mRNA capping
title_sort synthetic mrna capping
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753152/
https://www.ncbi.nlm.nih.gov/pubmed/30018667
http://dx.doi.org/10.3762/bjoc.13.274
work_keys_str_mv AT muttachfabian syntheticmrnacapping
AT muthmannnils syntheticmrnacapping
AT rentmeisterandrea syntheticmrnacapping

Ejemplares similares