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Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells

One of the serious obstacles of the aortopathies research is a considerable shortage of human aortic smooth muscle cells (SMCs), which can be used to model the disease. SMC in most cases come from the whole aorta of transplant donors, which are rather difficult to access. In the course of coronary a...

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Autores principales: Kostina, Daria, Zverev, Dmitry, Grebennik, Vadim, Gordeev, Mikhail, Ignatieva, Elena, Voronkina, Irina, Kostareva, Anna, Malashicheva, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753977/
https://www.ncbi.nlm.nih.gov/pubmed/29251110
http://dx.doi.org/10.1177/0963689717721226
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author Kostina, Daria
Zverev, Dmitry
Grebennik, Vadim
Gordeev, Mikhail
Ignatieva, Elena
Voronkina, Irina
Kostareva, Anna
Malashicheva, Anna
author_facet Kostina, Daria
Zverev, Dmitry
Grebennik, Vadim
Gordeev, Mikhail
Ignatieva, Elena
Voronkina, Irina
Kostareva, Anna
Malashicheva, Anna
author_sort Kostina, Daria
collection PubMed
description One of the serious obstacles of the aortopathies research is a considerable shortage of human aortic smooth muscle cells (SMCs), which can be used to model the disease. SMC in most cases come from the whole aorta of transplant donors, which are rather difficult to access. In the course of coronary artery bypass graft (CABG) surgery, a fragment of aortic tissue is excised to make a bypass root. In this study, we show a possibility to use CABG leftover fragments of thoracic aorta as a source of human SMC for in vitro research. We isolated SMC from the fragments of aortic tissues obtained during CABG procedure and compared these cells to the cells that were isolated from aortic tissue of transplant donors. The content of key SMC contractile markers (SMA, SM22α, and vimentin) as well as proliferation and migration rates, metalloproteases MMP-2 and MMP-9 activities were similar in CABG-derived SMC and in transplant donor–derived SMC. In conclusion, leftovers of ascending thoracic aorta obtained during CABG can be used as a source of human aortic SMCs for in vitro research.
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spelling pubmed-57539772018-01-09 Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells Kostina, Daria Zverev, Dmitry Grebennik, Vadim Gordeev, Mikhail Ignatieva, Elena Voronkina, Irina Kostareva, Anna Malashicheva, Anna Cell Transplant Original Articles One of the serious obstacles of the aortopathies research is a considerable shortage of human aortic smooth muscle cells (SMCs), which can be used to model the disease. SMC in most cases come from the whole aorta of transplant donors, which are rather difficult to access. In the course of coronary artery bypass graft (CABG) surgery, a fragment of aortic tissue is excised to make a bypass root. In this study, we show a possibility to use CABG leftover fragments of thoracic aorta as a source of human SMC for in vitro research. We isolated SMC from the fragments of aortic tissues obtained during CABG procedure and compared these cells to the cells that were isolated from aortic tissue of transplant donors. The content of key SMC contractile markers (SMA, SM22α, and vimentin) as well as proliferation and migration rates, metalloproteases MMP-2 and MMP-9 activities were similar in CABG-derived SMC and in transplant donor–derived SMC. In conclusion, leftovers of ascending thoracic aorta obtained during CABG can be used as a source of human aortic SMCs for in vitro research. SAGE Publications 2017-12-18 2017-10 /pmc/articles/PMC5753977/ /pubmed/29251110 http://dx.doi.org/10.1177/0963689717721226 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Kostina, Daria
Zverev, Dmitry
Grebennik, Vadim
Gordeev, Mikhail
Ignatieva, Elena
Voronkina, Irina
Kostareva, Anna
Malashicheva, Anna
Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells
title Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells
title_full Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells
title_fullStr Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells
title_full_unstemmed Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells
title_short Aortic Graft at Coronary Artery Bypass Surgery as a Source of Human Aortic Smooth Muscle Cells
title_sort aortic graft at coronary artery bypass surgery as a source of human aortic smooth muscle cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753977/
https://www.ncbi.nlm.nih.gov/pubmed/29251110
http://dx.doi.org/10.1177/0963689717721226
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