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Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings
Newborns are at increased risk of jaundice, a condition in which excess bilirubin accumulates in blood. Left untreated, jaundice can lead to neurological impairment and death. Jaundice resulting from unconjugated hyperbilirubinemia is easily treated with exposure to blue light, and phototherapy syst...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754796/ https://www.ncbi.nlm.nih.gov/pubmed/29203650 http://dx.doi.org/10.1073/pnas.1714020114 |
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author | Keahey, Pelham A. Simeral, Mathieu L. Schroder, Kristofer J. Bond, Meaghan M. Mtenthaonnga, Prince J. Miros, Robert H. Dube, Queen Richards-Kortum, Rebecca R. |
author_facet | Keahey, Pelham A. Simeral, Mathieu L. Schroder, Kristofer J. Bond, Meaghan M. Mtenthaonnga, Prince J. Miros, Robert H. Dube, Queen Richards-Kortum, Rebecca R. |
author_sort | Keahey, Pelham A. |
collection | PubMed |
description | Newborns are at increased risk of jaundice, a condition in which excess bilirubin accumulates in blood. Left untreated, jaundice can lead to neurological impairment and death. Jaundice resulting from unconjugated hyperbilirubinemia is easily treated with exposure to blue light, and phototherapy systems have been developed for low-resource settings; however, there are no appropriate solutions to diagnose and monitor jaundice in these settings. To address this need we present BiliSpec, a low-cost reader and disposable lateral flow card designed to measure the concentration of total bilirubin from several drops of blood at the point of care. We evaluated the performance of BiliSpec, using blood from normal volunteers spiked with varying amounts of bilirubin; results measured using BiliSpec correlated well with a reference laboratory bilirubinometer (r = 0.996). We then performed a pilot clinical study using BiliSpec to measure total bilirubin in neonates at risk for jaundice at Queen Elizabeth Central Hospital in Blantyre, Malawi. Concentrations measured using BiliSpec correlated well with those measured using a laboratory reference standard in 94 patient samples ranging from 1.1 mg/dL to 23.0 mg/dL in concentration (r = 0.973). The mean difference between bilirubin levels measured with BiliSpec and the reference standard was 0.3 mg/dL (95 [Formula: see text] CI: −1.7–2.2 mg/dL). |
format | Online Article Text |
id | pubmed-5754796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-57547962018-01-08 Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings Keahey, Pelham A. Simeral, Mathieu L. Schroder, Kristofer J. Bond, Meaghan M. Mtenthaonnga, Prince J. Miros, Robert H. Dube, Queen Richards-Kortum, Rebecca R. Proc Natl Acad Sci U S A PNAS Plus Newborns are at increased risk of jaundice, a condition in which excess bilirubin accumulates in blood. Left untreated, jaundice can lead to neurological impairment and death. Jaundice resulting from unconjugated hyperbilirubinemia is easily treated with exposure to blue light, and phototherapy systems have been developed for low-resource settings; however, there are no appropriate solutions to diagnose and monitor jaundice in these settings. To address this need we present BiliSpec, a low-cost reader and disposable lateral flow card designed to measure the concentration of total bilirubin from several drops of blood at the point of care. We evaluated the performance of BiliSpec, using blood from normal volunteers spiked with varying amounts of bilirubin; results measured using BiliSpec correlated well with a reference laboratory bilirubinometer (r = 0.996). We then performed a pilot clinical study using BiliSpec to measure total bilirubin in neonates at risk for jaundice at Queen Elizabeth Central Hospital in Blantyre, Malawi. Concentrations measured using BiliSpec correlated well with those measured using a laboratory reference standard in 94 patient samples ranging from 1.1 mg/dL to 23.0 mg/dL in concentration (r = 0.973). The mean difference between bilirubin levels measured with BiliSpec and the reference standard was 0.3 mg/dL (95 [Formula: see text] CI: −1.7–2.2 mg/dL). National Academy of Sciences 2017-12-19 2017-12-04 /pmc/articles/PMC5754796/ /pubmed/29203650 http://dx.doi.org/10.1073/pnas.1714020114 Text en Copyright © 2017 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | PNAS Plus Keahey, Pelham A. Simeral, Mathieu L. Schroder, Kristofer J. Bond, Meaghan M. Mtenthaonnga, Prince J. Miros, Robert H. Dube, Queen Richards-Kortum, Rebecca R. Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
title | Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
title_full | Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
title_fullStr | Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
title_full_unstemmed | Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
title_short | Point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
title_sort | point-of-care device to diagnose and monitor neonatal jaundice in low-resource settings |
topic | PNAS Plus |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754796/ https://www.ncbi.nlm.nih.gov/pubmed/29203650 http://dx.doi.org/10.1073/pnas.1714020114 |
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