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Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway

This study investigated the effect of metformin on chemosensitivity of hepatocarcinoma cells to cisplatin and the possible mechanism. HepG2 and Huh-7 hepatoma cells were treated with cisplatin at concentrations of 0, 2, 4, 6, 8 and 10 µM for 48 h. Proliferation of HepG2 and Huh-7 hepatoma cells were...

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Autores principales: Dong, Hao, Huang, Jungang, Zheng, Kang, Tan, Dong, Chang, Qi, Gong, Genqiang, Zhang, Qing, Tang, Hanqiu, Sun, Jianguo, Zhang, Shaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755027/
https://www.ncbi.nlm.nih.gov/pubmed/29344225
http://dx.doi.org/10.3892/ol.2017.7198
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author Dong, Hao
Huang, Jungang
Zheng, Kang
Tan, Dong
Chang, Qi
Gong, Genqiang
Zhang, Qing
Tang, Hanqiu
Sun, Jianguo
Zhang, Shaoyu
author_facet Dong, Hao
Huang, Jungang
Zheng, Kang
Tan, Dong
Chang, Qi
Gong, Genqiang
Zhang, Qing
Tang, Hanqiu
Sun, Jianguo
Zhang, Shaoyu
author_sort Dong, Hao
collection PubMed
description This study investigated the effect of metformin on chemosensitivity of hepatocarcinoma cells to cisplatin and the possible mechanism. HepG2 and Huh-7 hepatoma cells were treated with cisplatin at concentrations of 0, 2, 4, 6, 8 and 10 µM for 48 h. Proliferation of HepG2 and Huh-7 hepatoma cells were detected by MTT assay. Apoptosis of hepatocellular carcinoma cells was detected by flow cytometry. Western blot analysis was used to detect the expression of 5-monophosphate-activated protein kinase (AMPK) and p-AMPK protein. Proliferative activity of HepG2 and Huh-7 cells decreased with the increase of cisplatin concentration. After adding metformin, proliferation ability of hepatocarcinoma cells was significantly reduced. Apoptosis rate of the metformin was significantly higher than that of the control group, and apoptosis rate of the cisplatin + metformin was significantly higher than that of the cisplatin group. There was no significant difference in expression level of AMPK protein found between control, metformin, cisplatin and cisplatin + metformin group. Compared with the control, ratio of p-AMPK/AMPK in metformin group was increased, and ratio of p-AMPK/AMPK in cisplatin + metformin was significantly higher than that in cisplatin group. Activity of cells in cisplatin + metformin + compound C (AMPK pathway blocker) group was significantly higher than that of cisplatin + metformin, while apoptosis of cells in cisplatin + metformin + compound C (AMPK pathway blocker) was significantly lower than that of cisplatin + metformin group. In conclusion, metformin can inhibit the proliferation, promote apoptosis and enhance the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway.
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spelling pubmed-57550272018-01-17 Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway Dong, Hao Huang, Jungang Zheng, Kang Tan, Dong Chang, Qi Gong, Genqiang Zhang, Qing Tang, Hanqiu Sun, Jianguo Zhang, Shaoyu Oncol Lett Articles This study investigated the effect of metformin on chemosensitivity of hepatocarcinoma cells to cisplatin and the possible mechanism. HepG2 and Huh-7 hepatoma cells were treated with cisplatin at concentrations of 0, 2, 4, 6, 8 and 10 µM for 48 h. Proliferation of HepG2 and Huh-7 hepatoma cells were detected by MTT assay. Apoptosis of hepatocellular carcinoma cells was detected by flow cytometry. Western blot analysis was used to detect the expression of 5-monophosphate-activated protein kinase (AMPK) and p-AMPK protein. Proliferative activity of HepG2 and Huh-7 cells decreased with the increase of cisplatin concentration. After adding metformin, proliferation ability of hepatocarcinoma cells was significantly reduced. Apoptosis rate of the metformin was significantly higher than that of the control group, and apoptosis rate of the cisplatin + metformin was significantly higher than that of the cisplatin group. There was no significant difference in expression level of AMPK protein found between control, metformin, cisplatin and cisplatin + metformin group. Compared with the control, ratio of p-AMPK/AMPK in metformin group was increased, and ratio of p-AMPK/AMPK in cisplatin + metformin was significantly higher than that in cisplatin group. Activity of cells in cisplatin + metformin + compound C (AMPK pathway blocker) group was significantly higher than that of cisplatin + metformin, while apoptosis of cells in cisplatin + metformin + compound C (AMPK pathway blocker) was significantly lower than that of cisplatin + metformin group. In conclusion, metformin can inhibit the proliferation, promote apoptosis and enhance the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway. D.A. Spandidos 2017-12 2017-10-17 /pmc/articles/PMC5755027/ /pubmed/29344225 http://dx.doi.org/10.3892/ol.2017.7198 Text en Copyright: © Dong et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Dong, Hao
Huang, Jungang
Zheng, Kang
Tan, Dong
Chang, Qi
Gong, Genqiang
Zhang, Qing
Tang, Hanqiu
Sun, Jianguo
Zhang, Shaoyu
Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway
title Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway
title_full Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway
title_fullStr Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway
title_full_unstemmed Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway
title_short Metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through AMPK pathway
title_sort metformin enhances the chemosensitivity of hepatocarcinoma cells to cisplatin through ampk pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755027/
https://www.ncbi.nlm.nih.gov/pubmed/29344225
http://dx.doi.org/10.3892/ol.2017.7198
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