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ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition

All-trans-retinoic acid (ATRA) can enhance iodine uptake capability of thyroid tumors, but the mechanisms remain poorly understood. The aim of the present study was to investigate the effects of ATRA on isotope susceptibility, proliferation and invasion of anaplastic thyroid carcinoma (ATC) and pote...

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Autores principales: Lan, Ling, Basourakos, Spyros, Cui, Dai, Zuo, Xuemei, Deng, Wei, Huo, Lili, Chen, Hailing, Zhang, Guoying, Deng, Lili, Shi, Bingyin, Luo, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755144/
https://www.ncbi.nlm.nih.gov/pubmed/29344218
http://dx.doi.org/10.3892/ol.2017.7225
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author Lan, Ling
Basourakos, Spyros
Cui, Dai
Zuo, Xuemei
Deng, Wei
Huo, Lili
Chen, Hailing
Zhang, Guoying
Deng, Lili
Shi, Bingyin
Luo, Yong
author_facet Lan, Ling
Basourakos, Spyros
Cui, Dai
Zuo, Xuemei
Deng, Wei
Huo, Lili
Chen, Hailing
Zhang, Guoying
Deng, Lili
Shi, Bingyin
Luo, Yong
author_sort Lan, Ling
collection PubMed
description All-trans-retinoic acid (ATRA) can enhance iodine uptake capability of thyroid tumors, but the mechanisms remain poorly understood. The aim of the present study was to investigate the effects of ATRA on isotope susceptibility, proliferation and invasion of anaplastic thyroid carcinoma (ATC) and potential mechanisms. SW1736 cells were treated with 1 µmol/l ATRA or 1% ethanol for 5 days. A cell line stably expressing β-catenin-shRNA was established. An iodine uptake assay was performed using (125)I. Proliferation and invasiveness were tested using MTT and Transwell assays, respectively. Western blotting was used to assess the expression of β-catenin, glycogen synthase kinase-3β (GSK-3β), sodium/iodine symporter (NIS) and proteins involved in epithelial-mesenchymal transition. Cells pretreated with ATRA were injected subcutaneously into SCID mice. Mice were intraperitoneally injected with (131)I once on the first day of treatment, and tumor growth was then assessed. After 35 days of (131)I treatment, ATRA-pretreated tumor volume and weight were decreased compared with the (131)I alone group (163.32±19.57 vs. 332.06±21.37 mm(3); 0.35±0.14 vs. 0.67±0.23 g, both P<0.05). Similar results were observed in the β-catenin shRNA-pretreated tumors. ATRA also increased the uptake of iodine by SW1736 cells (P<0.01), and similar results were observed in β-catenin shRNA cells. ATRA treatment decreased the cell proliferation and invasion compared with control cells (all P<0.05), similar to β-catenin shRNA. ATRA treatment decreased the expression of phosphorylated (p-)β-catenin, p-GSK-3β, vimentin, and fibronectin, and increased the expression of NIS and E-cadherin, compared with the control. ATRA increased the iodine uptake and inhibited the proliferation and invasion of SW1736 cells, involving β-catenin phosphorylation. In conclusion, ATRA could be used to improve the isotope sensitivity of ATC.
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spelling pubmed-57551442018-01-17 ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition Lan, Ling Basourakos, Spyros Cui, Dai Zuo, Xuemei Deng, Wei Huo, Lili Chen, Hailing Zhang, Guoying Deng, Lili Shi, Bingyin Luo, Yong Oncol Lett Articles All-trans-retinoic acid (ATRA) can enhance iodine uptake capability of thyroid tumors, but the mechanisms remain poorly understood. The aim of the present study was to investigate the effects of ATRA on isotope susceptibility, proliferation and invasion of anaplastic thyroid carcinoma (ATC) and potential mechanisms. SW1736 cells were treated with 1 µmol/l ATRA or 1% ethanol for 5 days. A cell line stably expressing β-catenin-shRNA was established. An iodine uptake assay was performed using (125)I. Proliferation and invasiveness were tested using MTT and Transwell assays, respectively. Western blotting was used to assess the expression of β-catenin, glycogen synthase kinase-3β (GSK-3β), sodium/iodine symporter (NIS) and proteins involved in epithelial-mesenchymal transition. Cells pretreated with ATRA were injected subcutaneously into SCID mice. Mice were intraperitoneally injected with (131)I once on the first day of treatment, and tumor growth was then assessed. After 35 days of (131)I treatment, ATRA-pretreated tumor volume and weight were decreased compared with the (131)I alone group (163.32±19.57 vs. 332.06±21.37 mm(3); 0.35±0.14 vs. 0.67±0.23 g, both P<0.05). Similar results were observed in the β-catenin shRNA-pretreated tumors. ATRA also increased the uptake of iodine by SW1736 cells (P<0.01), and similar results were observed in β-catenin shRNA cells. ATRA treatment decreased the cell proliferation and invasion compared with control cells (all P<0.05), similar to β-catenin shRNA. ATRA treatment decreased the expression of phosphorylated (p-)β-catenin, p-GSK-3β, vimentin, and fibronectin, and increased the expression of NIS and E-cadherin, compared with the control. ATRA increased the iodine uptake and inhibited the proliferation and invasion of SW1736 cells, involving β-catenin phosphorylation. In conclusion, ATRA could be used to improve the isotope sensitivity of ATC. D.A. Spandidos 2017-12 2017-10-19 /pmc/articles/PMC5755144/ /pubmed/29344218 http://dx.doi.org/10.3892/ol.2017.7225 Text en Copyright: © Lan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lan, Ling
Basourakos, Spyros
Cui, Dai
Zuo, Xuemei
Deng, Wei
Huo, Lili
Chen, Hailing
Zhang, Guoying
Deng, Lili
Shi, Bingyin
Luo, Yong
ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition
title ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition
title_full ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition
title_fullStr ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition
title_full_unstemmed ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition
title_short ATRA increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma SW1736 cells: Involvement of β-catenin phosphorylation inhibition
title_sort atra increases iodine uptake and inhibits the proliferation and invasiveness of human anaplastic thyroid carcinoma sw1736 cells: involvement of β-catenin phosphorylation inhibition
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755144/
https://www.ncbi.nlm.nih.gov/pubmed/29344218
http://dx.doi.org/10.3892/ol.2017.7225
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