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Hypermethylation downregulates P2X(7) receptor expression in astrocytoma
The present study investigated the altered expression of p2X purinoceptor (P2X(7)R) in astrocytoma. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to determine the P2X(7)R expression in glioblastoma (GBM) and surrounding normal brain tissue. DNA meth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755164/ https://www.ncbi.nlm.nih.gov/pubmed/29344216 http://dx.doi.org/10.3892/ol.2017.7241 |
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author | Liu, Jing Li, Ningning Sheng, Ruofan Wang, Rui Xu, Zude Mao, Ying Wang, Yin Liu, Ying |
author_facet | Liu, Jing Li, Ningning Sheng, Ruofan Wang, Rui Xu, Zude Mao, Ying Wang, Yin Liu, Ying |
author_sort | Liu, Jing |
collection | PubMed |
description | The present study investigated the altered expression of p2X purinoceptor (P2X(7)R) in astrocytoma. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to determine the P2X(7)R expression in glioblastoma (GBM) and surrounding normal brain tissue. DNA methylation levels of P2X(7)R gene promoter in GBM were analyzed using a Sequenom MassARRAY(®) System. Immunohistochemistry (IHC) was used to detect the expression of P2X(7)R in astrocytoma at different malignancy grades, including diffuse astrocytoma, anaplastic astrocytoma and GBM. P2X(7)R mRNA and protein were significantly decreased in GBM compared with normal brain tissues. IHC results showed a negative correlation between P2X(7)R expression and tumor grade. The decreased P2X(7)R expression was mostly attributed to hypermethylation of its promoter. Therefore, P2X(7)R was found to perform an important role in tumorigenesis and progression of astrocytoma. |
format | Online Article Text |
id | pubmed-5755164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57551642018-01-17 Hypermethylation downregulates P2X(7) receptor expression in astrocytoma Liu, Jing Li, Ningning Sheng, Ruofan Wang, Rui Xu, Zude Mao, Ying Wang, Yin Liu, Ying Oncol Lett Articles The present study investigated the altered expression of p2X purinoceptor (P2X(7)R) in astrocytoma. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to determine the P2X(7)R expression in glioblastoma (GBM) and surrounding normal brain tissue. DNA methylation levels of P2X(7)R gene promoter in GBM were analyzed using a Sequenom MassARRAY(®) System. Immunohistochemistry (IHC) was used to detect the expression of P2X(7)R in astrocytoma at different malignancy grades, including diffuse astrocytoma, anaplastic astrocytoma and GBM. P2X(7)R mRNA and protein were significantly decreased in GBM compared with normal brain tissues. IHC results showed a negative correlation between P2X(7)R expression and tumor grade. The decreased P2X(7)R expression was mostly attributed to hypermethylation of its promoter. Therefore, P2X(7)R was found to perform an important role in tumorigenesis and progression of astrocytoma. D.A. Spandidos 2017-12 2017-10-20 /pmc/articles/PMC5755164/ /pubmed/29344216 http://dx.doi.org/10.3892/ol.2017.7241 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Jing Li, Ningning Sheng, Ruofan Wang, Rui Xu, Zude Mao, Ying Wang, Yin Liu, Ying Hypermethylation downregulates P2X(7) receptor expression in astrocytoma |
title | Hypermethylation downregulates P2X(7) receptor expression in astrocytoma |
title_full | Hypermethylation downregulates P2X(7) receptor expression in astrocytoma |
title_fullStr | Hypermethylation downregulates P2X(7) receptor expression in astrocytoma |
title_full_unstemmed | Hypermethylation downregulates P2X(7) receptor expression in astrocytoma |
title_short | Hypermethylation downregulates P2X(7) receptor expression in astrocytoma |
title_sort | hypermethylation downregulates p2x(7) receptor expression in astrocytoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755164/ https://www.ncbi.nlm.nih.gov/pubmed/29344216 http://dx.doi.org/10.3892/ol.2017.7241 |
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