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Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers
The checkpoint with forkhead-associated (FHA) domain and RING-finger (CHFR) protein was identified as a cell cycle checkpoint protein and E3 ubiquitin ligase. In the present study, the potential functions of CHFR in pancreatic cancer were investigated. CHFR expression was measured in five pancreatic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755226/ https://www.ncbi.nlm.nih.gov/pubmed/29344247 http://dx.doi.org/10.3892/ol.2017.7239 |
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author | Zhang, Di Xu, Xiao-Lan Li, Fei Sun, Hai-Chen Cui, Ye-Qing Liu, Shuang Xu, Ping-Yong |
author_facet | Zhang, Di Xu, Xiao-Lan Li, Fei Sun, Hai-Chen Cui, Ye-Qing Liu, Shuang Xu, Ping-Yong |
author_sort | Zhang, Di |
collection | PubMed |
description | The checkpoint with forkhead-associated (FHA) domain and RING-finger (CHFR) protein was identified as a cell cycle checkpoint protein and E3 ubiquitin ligase. In the present study, the potential functions of CHFR in pancreatic cancer were investigated. CHFR expression was measured in five pancreatic cancer cell lines by reverse transcription- quantitative polymerase chain reaction and western blotting. Capan-1 cells stably expressing CHFR were established by lentiviral vector transfection. Cell proliferation was assessed using Cell Counting Kit-8, and cell migration/invasion assay was determined using Transwell assays. Cell cycle and apoptosis induced by gemcitabine or docetaxel were evaluated using flow cytometry. CHFR expression levels were also evaluated in pancreatic ductal adenocarcinoma (PDAC) tumor samples as well as adjacent non-tumor tissues by immunohistochemistry. The significance of CHFR expression was determined, with respect to clinicopathological features and overall survival. Overexpression of CHFR in Capan-1 cells led to a decreased proliferative rate and reduced cell migration and invasion abilities. Results also indicated an increase in G1 phase cells in Capan-1 cells overexpressing CHFR. Docetaxel-induced apoptosis was inhibited in Capan-1 cells with CHFR-overexpression. A reduction in CHFR expression was detected in 51.9% of patients with PDAC, which significantly correlated with later T-stage. The results show CHFR functions as a tumor suppressor in pancreatic cancer, suggests its potential role in controlling the cell cycle of pancreatic cancer cells; however, CHFR overexpression is not a favorable factor in apoptosis induced by docetaxel. |
format | Online Article Text |
id | pubmed-5755226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57552262018-01-17 Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers Zhang, Di Xu, Xiao-Lan Li, Fei Sun, Hai-Chen Cui, Ye-Qing Liu, Shuang Xu, Ping-Yong Oncol Lett Articles The checkpoint with forkhead-associated (FHA) domain and RING-finger (CHFR) protein was identified as a cell cycle checkpoint protein and E3 ubiquitin ligase. In the present study, the potential functions of CHFR in pancreatic cancer were investigated. CHFR expression was measured in five pancreatic cancer cell lines by reverse transcription- quantitative polymerase chain reaction and western blotting. Capan-1 cells stably expressing CHFR were established by lentiviral vector transfection. Cell proliferation was assessed using Cell Counting Kit-8, and cell migration/invasion assay was determined using Transwell assays. Cell cycle and apoptosis induced by gemcitabine or docetaxel were evaluated using flow cytometry. CHFR expression levels were also evaluated in pancreatic ductal adenocarcinoma (PDAC) tumor samples as well as adjacent non-tumor tissues by immunohistochemistry. The significance of CHFR expression was determined, with respect to clinicopathological features and overall survival. Overexpression of CHFR in Capan-1 cells led to a decreased proliferative rate and reduced cell migration and invasion abilities. Results also indicated an increase in G1 phase cells in Capan-1 cells overexpressing CHFR. Docetaxel-induced apoptosis was inhibited in Capan-1 cells with CHFR-overexpression. A reduction in CHFR expression was detected in 51.9% of patients with PDAC, which significantly correlated with later T-stage. The results show CHFR functions as a tumor suppressor in pancreatic cancer, suggests its potential role in controlling the cell cycle of pancreatic cancer cells; however, CHFR overexpression is not a favorable factor in apoptosis induced by docetaxel. D.A. Spandidos 2017-12 2017-10-20 /pmc/articles/PMC5755226/ /pubmed/29344247 http://dx.doi.org/10.3892/ol.2017.7239 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Di Xu, Xiao-Lan Li, Fei Sun, Hai-Chen Cui, Ye-Qing Liu, Shuang Xu, Ping-Yong Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers |
title | Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers |
title_full | Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers |
title_fullStr | Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers |
title_full_unstemmed | Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers |
title_short | Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers |
title_sort | upregulation of the checkpoint protein chfr is associated with tumor suppression in pancreatic cancers |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755226/ https://www.ncbi.nlm.nih.gov/pubmed/29344247 http://dx.doi.org/10.3892/ol.2017.7239 |
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