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Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
Blood can be collected from rats in a number of sampling locations. For instance, the tail vein is a superior location for blood sampling. However, the tail vein is thin so that it is sometimes hard to puncture. In addition, the tail vein has low blood flow and requires a long sampling time to get s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755556/ https://www.ncbi.nlm.nih.gov/pubmed/29286462 http://dx.doi.org/10.3791/55852 |
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author | Zou, Wusong Yang, Yanqun Gu, Ye Zhu, Pengfei Zhang, Mingjing Cheng, Zhong Liu, Xiaoyan Yu, Yijun Peng, Xiaohong |
author_facet | Zou, Wusong Yang, Yanqun Gu, Ye Zhu, Pengfei Zhang, Mingjing Cheng, Zhong Liu, Xiaoyan Yu, Yijun Peng, Xiaohong |
author_sort | Zou, Wusong |
collection | PubMed |
description | Blood can be collected from rats in a number of sampling locations. For instance, the tail vein is a superior location for blood sampling. However, the tail vein is thin so that it is sometimes hard to puncture. In addition, the tail vein has low blood flow and requires a long sampling time to get sufficient blood. The present report describes a simple blood sampling method, the vacuum blood collection method, which is usually used to obtain blood samples from patients, here used for non-anesthetized rats. The 22 G butterfly needle tip was inserted into one of the lateral tail veins approximately 2-3 cm from the tip of the tail at an angle of approximately 20°, and blood was collected in the vacuum collection tube by inserting the rubber end of the butterfly needle into the vacuum blood collection tube. The present experimental results show that the success rate was 95% in the experimental group and 90% in the beginner group. The success rate and puncture times were similar between two groups. The sampling duration was significantly shorter in the experimental group compared to beginner group. In conclusion, this vacuum blood collection method for sequential blood sampling from the tail vein of non- anesthetized rats is feasible and easy-to-learn, which might serve as a reliable alternative to other conventionally used blood sampling techniques for rats. |
format | Online Article Text |
id | pubmed-5755556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-57555562018-01-19 Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System Zou, Wusong Yang, Yanqun Gu, Ye Zhu, Pengfei Zhang, Mingjing Cheng, Zhong Liu, Xiaoyan Yu, Yijun Peng, Xiaohong J Vis Exp Medicine Blood can be collected from rats in a number of sampling locations. For instance, the tail vein is a superior location for blood sampling. However, the tail vein is thin so that it is sometimes hard to puncture. In addition, the tail vein has low blood flow and requires a long sampling time to get sufficient blood. The present report describes a simple blood sampling method, the vacuum blood collection method, which is usually used to obtain blood samples from patients, here used for non-anesthetized rats. The 22 G butterfly needle tip was inserted into one of the lateral tail veins approximately 2-3 cm from the tip of the tail at an angle of approximately 20°, and blood was collected in the vacuum collection tube by inserting the rubber end of the butterfly needle into the vacuum blood collection tube. The present experimental results show that the success rate was 95% in the experimental group and 90% in the beginner group. The success rate and puncture times were similar between two groups. The sampling duration was significantly shorter in the experimental group compared to beginner group. In conclusion, this vacuum blood collection method for sequential blood sampling from the tail vein of non- anesthetized rats is feasible and easy-to-learn, which might serve as a reliable alternative to other conventionally used blood sampling techniques for rats. MyJove Corporation 2017-12-10 /pmc/articles/PMC5755556/ /pubmed/29286462 http://dx.doi.org/10.3791/55852 Text en Copyright © 2017, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Medicine Zou, Wusong Yang, Yanqun Gu, Ye Zhu, Pengfei Zhang, Mingjing Cheng, Zhong Liu, Xiaoyan Yu, Yijun Peng, Xiaohong Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System |
title | Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System |
title_full | Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System |
title_fullStr | Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System |
title_full_unstemmed | Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System |
title_short | Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System |
title_sort | repeated blood collection from tail vein of non-anesthetized rats with a vacuum blood collection system |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755556/ https://www.ncbi.nlm.nih.gov/pubmed/29286462 http://dx.doi.org/10.3791/55852 |
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