Cargando…
The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women
AIM: To assess the association of GLO1 C332C gene polymorphism with breast cancer risk at different stages of the disease and to investigate the effect of this gene polymorphism on its mRNA expression and enzyme activity. METHODS: GLO1 C332C gene polymorphism was analyzed by PCR-RFLP in 100 healthy...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755725/ https://www.ncbi.nlm.nih.gov/pubmed/29321821 http://dx.doi.org/10.18632/genesandcancer.163 |
_version_ | 1783290620742205440 |
---|---|
author | Abdul-Maksoud, Rehab S. Elsayed, Walid SH. Elsayed, Rasha S. |
author_facet | Abdul-Maksoud, Rehab S. Elsayed, Walid SH. Elsayed, Rasha S. |
author_sort | Abdul-Maksoud, Rehab S. |
collection | PubMed |
description | AIM: To assess the association of GLO1 C332C gene polymorphism with breast cancer risk at different stages of the disease and to investigate the effect of this gene polymorphism on its mRNA expression and enzyme activity. METHODS: GLO1 C332C gene polymorphism was analyzed by PCR-RFLP in 100 healthy controls and 200 patients with breast cancer (100 patients with stage I & II and 100 patients with stage III & IV). GLO1 mRNA expression was measured by real time PCR. Serum GLO1 enzyme activity was measured colorimetrically. RESULTS: GLO1 A allele was associated with increased risk of breast cancer [OR (95%CI)= 2.8(1.9-4.1), P < 0.001]. Its frequency was significantly higher among advanced stages of breast cancer compared with localized tumors (OR (95%CI)= 1.9(1.3-2.9), p < 0.001). GLO1 mRNA expression and enzyme activity were significantly higher in breast cancer patients compared to controls and they were much higher in the advanced stages of the disease (P < 0.001). Carriers of AA genotype showed higher GLO1 expression and enzyme activity compared with carriers of CC genotype. CONCLUSION: GLO1 C332C SNP was associated with overexpression of GLO1 mRNA and higher enzyme activity in breast cancer patients suggesting its role in the development of breast cancer and its progression from localized to advanced. |
format | Online Article Text |
id | pubmed-5755725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57557252018-01-10 The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women Abdul-Maksoud, Rehab S. Elsayed, Walid SH. Elsayed, Rasha S. Genes Cancer Research Paper AIM: To assess the association of GLO1 C332C gene polymorphism with breast cancer risk at different stages of the disease and to investigate the effect of this gene polymorphism on its mRNA expression and enzyme activity. METHODS: GLO1 C332C gene polymorphism was analyzed by PCR-RFLP in 100 healthy controls and 200 patients with breast cancer (100 patients with stage I & II and 100 patients with stage III & IV). GLO1 mRNA expression was measured by real time PCR. Serum GLO1 enzyme activity was measured colorimetrically. RESULTS: GLO1 A allele was associated with increased risk of breast cancer [OR (95%CI)= 2.8(1.9-4.1), P < 0.001]. Its frequency was significantly higher among advanced stages of breast cancer compared with localized tumors (OR (95%CI)= 1.9(1.3-2.9), p < 0.001). GLO1 mRNA expression and enzyme activity were significantly higher in breast cancer patients compared to controls and they were much higher in the advanced stages of the disease (P < 0.001). Carriers of AA genotype showed higher GLO1 expression and enzyme activity compared with carriers of CC genotype. CONCLUSION: GLO1 C332C SNP was associated with overexpression of GLO1 mRNA and higher enzyme activity in breast cancer patients suggesting its role in the development of breast cancer and its progression from localized to advanced. Impact Journals LLC 2017-11 /pmc/articles/PMC5755725/ /pubmed/29321821 http://dx.doi.org/10.18632/genesandcancer.163 Text en Copyright: © 2017 Abdul-Maksoud et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Abdul-Maksoud, Rehab S. Elsayed, Walid SH. Elsayed, Rasha S. The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women |
title | The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women |
title_full | The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women |
title_fullStr | The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women |
title_full_unstemmed | The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women |
title_short | The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women |
title_sort | influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in egyptian women |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755725/ https://www.ncbi.nlm.nih.gov/pubmed/29321821 http://dx.doi.org/10.18632/genesandcancer.163 |
work_keys_str_mv | AT abdulmaksoudrehabs theinfluenceofglyoxalase1genepolymorphismonitsexpressionatdifferentstagesofbreastcancerinegyptianwomen AT elsayedwalidsh theinfluenceofglyoxalase1genepolymorphismonitsexpressionatdifferentstagesofbreastcancerinegyptianwomen AT elsayedrashas theinfluenceofglyoxalase1genepolymorphismonitsexpressionatdifferentstagesofbreastcancerinegyptianwomen AT abdulmaksoudrehabs influenceofglyoxalase1genepolymorphismonitsexpressionatdifferentstagesofbreastcancerinegyptianwomen AT elsayedwalidsh influenceofglyoxalase1genepolymorphismonitsexpressionatdifferentstagesofbreastcancerinegyptianwomen AT elsayedrashas influenceofglyoxalase1genepolymorphismonitsexpressionatdifferentstagesofbreastcancerinegyptianwomen |