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Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients
BACKGROUND: Most hypertensive patients, despite a proper control of their cardiovascular risk factors, have cardiovascular complications, evidencing the importance of controlling and/or reversing target-organ damage. In this sense, endothelial dysfunction has been associated with the presence of car...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755814/ https://www.ncbi.nlm.nih.gov/pubmed/29304136 http://dx.doi.org/10.1371/journal.pone.0190494 |
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author | Maroun-Eid, Charbel Ortega-Hernández, Adriana Modrego, Javier Abad-Cardiel, María García-Donaire, José Antonio Reinares, Leonardo Martell-Claros, Nieves Gómez-Garre, Dulcenombre |
author_facet | Maroun-Eid, Charbel Ortega-Hernández, Adriana Modrego, Javier Abad-Cardiel, María García-Donaire, José Antonio Reinares, Leonardo Martell-Claros, Nieves Gómez-Garre, Dulcenombre |
author_sort | Maroun-Eid, Charbel |
collection | PubMed |
description | BACKGROUND: Most hypertensive patients, despite a proper control of their cardiovascular risk factors, have cardiovascular complications, evidencing the importance of controlling and/or reversing target-organ damage. In this sense, endothelial dysfunction has been associated with the presence of cardiovascular risk factors and related cardiovascular outcomes. Since hypertension often clusters with other risk factors such as dyslipemia, diabetes and obesity, in this study we have investigated the effect of intensive multifactorial treatment on circulating vascular progenitor cell levels on high-risk hypertensive patients. DESIGN: We included108 hypertensive patients receiving intensive multifactorial pharmacologic treatment and dietary recommendations targeting blood pressure, dyslipemia, hyperglycemia and weight for 12 months. After the treatment period, blood samples were collected and circulating levels of endothelial (CD34+/KDR+, CD34+/VE-cadherin+) and smooth muscle (CD14+/endoglin+) progenitor cells were identified by flow cytometry. Additionally, plasma concentration of vascular endothelial growth factor (VEGF) was determined by ELISA. RESULTS: Most hypertensive patients (61±12 years, 47% men) showed cardiovascular parameters within normal ranges at baseline. Moreover, body mass index and the majority of the biochemical parameters (systolic and diastolic blood pressure, fasting glucose, total cholesterol, HDL-c, LDL-c, creatinine and hs-CRP) significantly decreased overtime. After 12 months of intensive treatment, CD34+/KDR+ and CD14+/endoglin+ levels did not change, but CD34+/VE-cadherin+ cells increased significantly at month 12 [0.9(0.05–0.14)% vs 0.05(0.02–0.09)% P<0.05]. However, VEGF plasma concentration decreased significantly overtime [89.1(53.9–218.7) vs [66.2(47.5–104.6) pg/mL, P<0.05]. CONCLUSIONS: Long-term intensive treatment in hypertensive patients further improves cardiovascular risk and increases circulating EPCs, suggesting that these cells could be a therapeutic target. |
format | Online Article Text |
id | pubmed-5755814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57558142018-01-26 Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients Maroun-Eid, Charbel Ortega-Hernández, Adriana Modrego, Javier Abad-Cardiel, María García-Donaire, José Antonio Reinares, Leonardo Martell-Claros, Nieves Gómez-Garre, Dulcenombre PLoS One Research Article BACKGROUND: Most hypertensive patients, despite a proper control of their cardiovascular risk factors, have cardiovascular complications, evidencing the importance of controlling and/or reversing target-organ damage. In this sense, endothelial dysfunction has been associated with the presence of cardiovascular risk factors and related cardiovascular outcomes. Since hypertension often clusters with other risk factors such as dyslipemia, diabetes and obesity, in this study we have investigated the effect of intensive multifactorial treatment on circulating vascular progenitor cell levels on high-risk hypertensive patients. DESIGN: We included108 hypertensive patients receiving intensive multifactorial pharmacologic treatment and dietary recommendations targeting blood pressure, dyslipemia, hyperglycemia and weight for 12 months. After the treatment period, blood samples were collected and circulating levels of endothelial (CD34+/KDR+, CD34+/VE-cadherin+) and smooth muscle (CD14+/endoglin+) progenitor cells were identified by flow cytometry. Additionally, plasma concentration of vascular endothelial growth factor (VEGF) was determined by ELISA. RESULTS: Most hypertensive patients (61±12 years, 47% men) showed cardiovascular parameters within normal ranges at baseline. Moreover, body mass index and the majority of the biochemical parameters (systolic and diastolic blood pressure, fasting glucose, total cholesterol, HDL-c, LDL-c, creatinine and hs-CRP) significantly decreased overtime. After 12 months of intensive treatment, CD34+/KDR+ and CD14+/endoglin+ levels did not change, but CD34+/VE-cadherin+ cells increased significantly at month 12 [0.9(0.05–0.14)% vs 0.05(0.02–0.09)% P<0.05]. However, VEGF plasma concentration decreased significantly overtime [89.1(53.9–218.7) vs [66.2(47.5–104.6) pg/mL, P<0.05]. CONCLUSIONS: Long-term intensive treatment in hypertensive patients further improves cardiovascular risk and increases circulating EPCs, suggesting that these cells could be a therapeutic target. Public Library of Science 2018-01-05 /pmc/articles/PMC5755814/ /pubmed/29304136 http://dx.doi.org/10.1371/journal.pone.0190494 Text en © 2018 Maroun-Eid et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Maroun-Eid, Charbel Ortega-Hernández, Adriana Modrego, Javier Abad-Cardiel, María García-Donaire, José Antonio Reinares, Leonardo Martell-Claros, Nieves Gómez-Garre, Dulcenombre Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
title | Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
title_full | Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
title_fullStr | Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
title_full_unstemmed | Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
title_short | Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
title_sort | effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755814/ https://www.ncbi.nlm.nih.gov/pubmed/29304136 http://dx.doi.org/10.1371/journal.pone.0190494 |
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