Cargando…
Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency
OBJECTIVE: We previously reported that patients with cholesteryl ester transfer protein (CETP) deficiency (CETP-D) have a higher prevalence of atherosclerotic cardiovascular disease, in spite of increased HDL-C levels. However, characterization of HDL in CETP-D has not been well described. Therefore...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755928/ https://www.ncbi.nlm.nih.gov/pubmed/29304079 http://dx.doi.org/10.1371/journal.pone.0190875 |
_version_ | 1783290659788029952 |
---|---|
author | Okada, Takeshi Ohama, Tohru Okazaki, Mitsuyo Kanno, Kotaro Matsuda, Hibiki Sairyo, Masami Zhu, Yinghong Saga, Ayami Kobayashi, Takuya Masuda, Daisaku Koseki, Masahiro Nishida, Makoto Sakata, Yasushi Yamashita, Shizuya |
author_facet | Okada, Takeshi Ohama, Tohru Okazaki, Mitsuyo Kanno, Kotaro Matsuda, Hibiki Sairyo, Masami Zhu, Yinghong Saga, Ayami Kobayashi, Takuya Masuda, Daisaku Koseki, Masahiro Nishida, Makoto Sakata, Yasushi Yamashita, Shizuya |
author_sort | Okada, Takeshi |
collection | PubMed |
description | OBJECTIVE: We previously reported that patients with cholesteryl ester transfer protein (CETP) deficiency (CETP-D) have a higher prevalence of atherosclerotic cardiovascular disease, in spite of increased HDL-C levels. However, characterization of HDL in CETP-D has not been well described. Therefore, we examined HDL particle number (PN) rather than HDL-C level. APPROACH AND RESULTS: Nine patients with CETP-D and 9 normolipidemic subjects were enrolled. We performed gel permeation high-performance liquid chromatography (GP-HPLC) analysis, determined the cholesterol and triglyceride composition of all lipoprotein subclasses, and calculated the PN of each subclass, which consisted of 3 VLDL (large, medium, and small), 4 LDL (large, medium, small, and very small), and 5 HDL (very large, large, medium, small, and very small) subclasses. The PNs of large and medium LDL were significantly lower in CETP-D than that in healthy subjects (0.66- and 0.63-fold decrease, respectively; p<0.001), whereas the PN of very small LDL, which is known to be atherogenic, was significantly higher (1.36-fold increase, p = 0.016). The PNs of very large and large HDL in CETP-D were markedly higher than that in healthy subjects (19.9- and 4.5-fold increase, respectively; p<0.001), whereas the PNs of small and very small HDL, which have more potent anti-atherogenic functions, were significantly lower (0.76- and 0.61-fold decrease, respectively; p<0.001). CONCLUSION: We have assessed the PNs of detailed subclasses of patients with CETP-D for the first time. The PN of larger HDL was markedly increased, that of smaller HDL was decreased, and that of very small LDL was increased, suggesting that CETP-D has pro-atherogenic lipoprotein properties. |
format | Online Article Text |
id | pubmed-5755928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57559282018-01-26 Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency Okada, Takeshi Ohama, Tohru Okazaki, Mitsuyo Kanno, Kotaro Matsuda, Hibiki Sairyo, Masami Zhu, Yinghong Saga, Ayami Kobayashi, Takuya Masuda, Daisaku Koseki, Masahiro Nishida, Makoto Sakata, Yasushi Yamashita, Shizuya PLoS One Research Article OBJECTIVE: We previously reported that patients with cholesteryl ester transfer protein (CETP) deficiency (CETP-D) have a higher prevalence of atherosclerotic cardiovascular disease, in spite of increased HDL-C levels. However, characterization of HDL in CETP-D has not been well described. Therefore, we examined HDL particle number (PN) rather than HDL-C level. APPROACH AND RESULTS: Nine patients with CETP-D and 9 normolipidemic subjects were enrolled. We performed gel permeation high-performance liquid chromatography (GP-HPLC) analysis, determined the cholesterol and triglyceride composition of all lipoprotein subclasses, and calculated the PN of each subclass, which consisted of 3 VLDL (large, medium, and small), 4 LDL (large, medium, small, and very small), and 5 HDL (very large, large, medium, small, and very small) subclasses. The PNs of large and medium LDL were significantly lower in CETP-D than that in healthy subjects (0.66- and 0.63-fold decrease, respectively; p<0.001), whereas the PN of very small LDL, which is known to be atherogenic, was significantly higher (1.36-fold increase, p = 0.016). The PNs of very large and large HDL in CETP-D were markedly higher than that in healthy subjects (19.9- and 4.5-fold increase, respectively; p<0.001), whereas the PNs of small and very small HDL, which have more potent anti-atherogenic functions, were significantly lower (0.76- and 0.61-fold decrease, respectively; p<0.001). CONCLUSION: We have assessed the PNs of detailed subclasses of patients with CETP-D for the first time. The PN of larger HDL was markedly increased, that of smaller HDL was decreased, and that of very small LDL was increased, suggesting that CETP-D has pro-atherogenic lipoprotein properties. Public Library of Science 2018-01-05 /pmc/articles/PMC5755928/ /pubmed/29304079 http://dx.doi.org/10.1371/journal.pone.0190875 Text en © 2018 Okada et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Okada, Takeshi Ohama, Tohru Okazaki, Mitsuyo Kanno, Kotaro Matsuda, Hibiki Sairyo, Masami Zhu, Yinghong Saga, Ayami Kobayashi, Takuya Masuda, Daisaku Koseki, Masahiro Nishida, Makoto Sakata, Yasushi Yamashita, Shizuya Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency |
title | Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency |
title_full | Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency |
title_fullStr | Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency |
title_full_unstemmed | Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency |
title_short | Particle number analysis of lipoprotein subclasses by gel permeation HPLC in patients with cholesteryl ester transfer protein deficiency |
title_sort | particle number analysis of lipoprotein subclasses by gel permeation hplc in patients with cholesteryl ester transfer protein deficiency |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755928/ https://www.ncbi.nlm.nih.gov/pubmed/29304079 http://dx.doi.org/10.1371/journal.pone.0190875 |
work_keys_str_mv | AT okadatakeshi particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT ohamatohru particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT okazakimitsuyo particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT kannokotaro particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT matsudahibiki particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT sairyomasami particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT zhuyinghong particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT sagaayami particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT kobayashitakuya particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT masudadaisaku particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT kosekimasahiro particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT nishidamakoto particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT sakatayasushi particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency AT yamashitashizuya particlenumberanalysisoflipoproteinsubclassesbygelpermeationhplcinpatientswithcholesterylestertransferproteindeficiency |