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A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran

A total number of 450 blood samples were collected from 45 different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in 4.2%, while 8.9% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infecti...

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Autores principales: Rajabi, Sepideh, Esmaeilnejad, Bijan, Tavassoli, Mousa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756249/
https://www.ncbi.nlm.nih.gov/pubmed/29326788
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author Rajabi, Sepideh
Esmaeilnejad, Bijan
Tavassoli, Mousa
author_facet Rajabi, Sepideh
Esmaeilnejad, Bijan
Tavassoli, Mousa
author_sort Rajabi, Sepideh
collection PubMed
description A total number of 450 blood samples were collected from 45 different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in 4.2%, while 8.9% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infections were detected in 37/40 (92.5%) and 3/40 (7.5%) samples, respectively. 530 ticks of 10 Ixodid species were collected from the same cattle. Hyalomma anatolicum was the most prevalent tick species (19.9%). An expected 520 bp fragment of Babesia spp. was generated in 22 (48.8%) of Rhpicephalus annulatus, 18 (40.0%) of R. bursa and 12 (30.0%) R. sanguineus sensu lato. The mPCR findings revealed that all infected ticks including R. annulatus, R. bursa and R. sanguineus were totally infected with B. bigemina. The DNA amplification of B. bovis and B. bigemina in egg samples showed that only B. bigemina was detected in two specimens of R. annulatus. It could be concluded that B. bigemina was the dominant causative agent in this region but the evidence of B. bovis infection of cattle in a few cases was noted, as well. The results suggested that B. bigemina and B. bovis could be detected in the DNA extracted from R. annulatus, R. bursa and R. sanguineus sensu lato confirming previous reports. Since B. bigemina is transmitted transovarially by R. annulatus, it might act as an important vector for B. bigemina.
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spelling pubmed-57562492018-01-11 A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran Rajabi, Sepideh Esmaeilnejad, Bijan Tavassoli, Mousa Vet Res Forum Original Article A total number of 450 blood samples were collected from 45 different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in 4.2%, while 8.9% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infections were detected in 37/40 (92.5%) and 3/40 (7.5%) samples, respectively. 530 ticks of 10 Ixodid species were collected from the same cattle. Hyalomma anatolicum was the most prevalent tick species (19.9%). An expected 520 bp fragment of Babesia spp. was generated in 22 (48.8%) of Rhpicephalus annulatus, 18 (40.0%) of R. bursa and 12 (30.0%) R. sanguineus sensu lato. The mPCR findings revealed that all infected ticks including R. annulatus, R. bursa and R. sanguineus were totally infected with B. bigemina. The DNA amplification of B. bovis and B. bigemina in egg samples showed that only B. bigemina was detected in two specimens of R. annulatus. It could be concluded that B. bigemina was the dominant causative agent in this region but the evidence of B. bovis infection of cattle in a few cases was noted, as well. The results suggested that B. bigemina and B. bovis could be detected in the DNA extracted from R. annulatus, R. bursa and R. sanguineus sensu lato confirming previous reports. Since B. bigemina is transmitted transovarially by R. annulatus, it might act as an important vector for B. bigemina. Urmia University 2017 2017-12-15 /pmc/articles/PMC5756249/ /pubmed/29326788 Text en © 2017 Urmia University. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rajabi, Sepideh
Esmaeilnejad, Bijan
Tavassoli, Mousa
A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
title A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
title_full A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
title_fullStr A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
title_full_unstemmed A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
title_short A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
title_sort molecular study on babesia spp. in cattle and ticks in west-azerbaijan province, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756249/
https://www.ncbi.nlm.nih.gov/pubmed/29326788
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