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Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)

BACKGROUND: The aim of present study was to evaluate antileishmanial effects of Lavandula angustifolia (L. angustifolia) and Rosmarinus officinalis (R. officinalis) medicinal plants essential oils and nano-emulsions on Leishmania major (L. major). METHODS: The present study was performed in Leishman...

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Autores principales: SHOKRI, Azar, SAEEDI, Majid, FAKHAR, Mahdi, MORTEZA-SEMNANI, Katayoun, KEIGHOBADI, Masoud, HOSSEINI TESHNIZI, Saeed, KELIDARI, Hamid Reza, SADJADI, Siamak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756313/
https://www.ncbi.nlm.nih.gov/pubmed/29317888
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author SHOKRI, Azar
SAEEDI, Majid
FAKHAR, Mahdi
MORTEZA-SEMNANI, Katayoun
KEIGHOBADI, Masoud
HOSSEINI TESHNIZI, Saeed
KELIDARI, Hamid Reza
SADJADI, Siamak
author_facet SHOKRI, Azar
SAEEDI, Majid
FAKHAR, Mahdi
MORTEZA-SEMNANI, Katayoun
KEIGHOBADI, Masoud
HOSSEINI TESHNIZI, Saeed
KELIDARI, Hamid Reza
SADJADI, Siamak
author_sort SHOKRI, Azar
collection PubMed
description BACKGROUND: The aim of present study was to evaluate antileishmanial effects of Lavandula angustifolia (L. angustifolia) and Rosmarinus officinalis (R. officinalis) medicinal plants essential oils and nano-emulsions on Leishmania major (L. major). METHODS: The present study was performed in Leishmaniasis Reference Lab at Mazandaran University of Medical Sciences, Iran during 2016–2017. The IC50 values were calculated in both the promastigote and amastigote stages in J774 macrophage in comparison with meglumine antimoniate (MA) as positive control. In addition, cytotoxicity effects of essential oils and nano-emulsions prepared from both plants against macrophages were evaluated. RESULTS: Both essential oil and nano-emulsion of Lavander and Rosemary showed anti-leishmania activity on promastigote with IC(50)=0.11 μl/mL, IC(50)=0.26 μl/mL, and IC(50)=0.08 μl/mL respectively. Moreover, during amastigote assay, Lavander and Rosemary essential oils and nano-emulsion were effective at least in concentration of 0.12 μl/mL and 0.06 μl/mL (P=0.0001) respectively, on mean infected macrophages (MIR) and amastigotes in macrophages (P=0.0001). Additionally, cytotoxicity assay against macrophage revealed no toxicity on the host cells at IC(50) concentrations. CONCLUSION: The nano-emulsions of both plants were more effective than essential oil in both MIR and amastigote. However, in comparison with MA, the Lavander essential oil is more effective in reducing MIR. Rosemary nano-emulsion reduced MIR significantly more than MA in concentration of 0.25 μl/mL (P<0.001). Further investigations are recommended to evaluate the effect of these medicinal plants in murine models.
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spelling pubmed-57563132018-01-09 Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER) SHOKRI, Azar SAEEDI, Majid FAKHAR, Mahdi MORTEZA-SEMNANI, Katayoun KEIGHOBADI, Masoud HOSSEINI TESHNIZI, Saeed KELIDARI, Hamid Reza SADJADI, Siamak Iran J Parasitol Short Communication BACKGROUND: The aim of present study was to evaluate antileishmanial effects of Lavandula angustifolia (L. angustifolia) and Rosmarinus officinalis (R. officinalis) medicinal plants essential oils and nano-emulsions on Leishmania major (L. major). METHODS: The present study was performed in Leishmaniasis Reference Lab at Mazandaran University of Medical Sciences, Iran during 2016–2017. The IC50 values were calculated in both the promastigote and amastigote stages in J774 macrophage in comparison with meglumine antimoniate (MA) as positive control. In addition, cytotoxicity effects of essential oils and nano-emulsions prepared from both plants against macrophages were evaluated. RESULTS: Both essential oil and nano-emulsion of Lavander and Rosemary showed anti-leishmania activity on promastigote with IC(50)=0.11 μl/mL, IC(50)=0.26 μl/mL, and IC(50)=0.08 μl/mL respectively. Moreover, during amastigote assay, Lavander and Rosemary essential oils and nano-emulsion were effective at least in concentration of 0.12 μl/mL and 0.06 μl/mL (P=0.0001) respectively, on mean infected macrophages (MIR) and amastigotes in macrophages (P=0.0001). Additionally, cytotoxicity assay against macrophage revealed no toxicity on the host cells at IC(50) concentrations. CONCLUSION: The nano-emulsions of both plants were more effective than essential oil in both MIR and amastigote. However, in comparison with MA, the Lavander essential oil is more effective in reducing MIR. Rosemary nano-emulsion reduced MIR significantly more than MA in concentration of 0.25 μl/mL (P<0.001). Further investigations are recommended to evaluate the effect of these medicinal plants in murine models. Tehran University of Medical Sciences 2017 /pmc/articles/PMC5756313/ /pubmed/29317888 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
SHOKRI, Azar
SAEEDI, Majid
FAKHAR, Mahdi
MORTEZA-SEMNANI, Katayoun
KEIGHOBADI, Masoud
HOSSEINI TESHNIZI, Saeed
KELIDARI, Hamid Reza
SADJADI, Siamak
Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
title Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
title_full Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
title_fullStr Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
title_full_unstemmed Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
title_short Antileishmanial Activity of Lavandula angustifolia and Rosmarinus Officinalis Essential Oils and Nano-emulsions on Leishmania major (MRHO/IR/75/ER)
title_sort antileishmanial activity of lavandula angustifolia and rosmarinus officinalis essential oils and nano-emulsions on leishmania major (mrho/ir/75/er)
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756313/
https://www.ncbi.nlm.nih.gov/pubmed/29317888
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