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Metabolic capability and in situ activity of microorganisms in an oil reservoir
BACKGROUND: Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756336/ https://www.ncbi.nlm.nih.gov/pubmed/29304850 http://dx.doi.org/10.1186/s40168-017-0392-1 |
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author | Liu, Yi-Fan Galzerani, Daniela Domingos Mbadinga, Serge Maurice Zaramela, Livia S. Gu, Ji-Dong Mu, Bo-Zhong Zengler, Karsten |
author_facet | Liu, Yi-Fan Galzerani, Daniela Domingos Mbadinga, Serge Maurice Zaramela, Livia S. Gu, Ji-Dong Mu, Bo-Zhong Zengler, Karsten |
author_sort | Liu, Yi-Fan |
collection | PubMed |
description | BACKGROUND: Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microbes are actively participating in crude oil degradation in situ and to gain insight into what metabolic pathways they deploy. RESULTS: Here, we describe the metabolic potential and in situ activity of microbial communities obtained from the Jiangsu Oil Reservoir (China) by an integrated metagenomics and metatranscriptomics approach. Almost complete genome sequences obtained by differential binning highlight the distinct capability of different community members to degrade hydrocarbons under oxic or anoxic condition. Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. Furthermore, nutritional requirements based on amino acid and vitamin auxotrophies suggest a complex network of interactions and dependencies among active community members that go beyond classical syntrophic exchanges; this network defines community composition and microbial ecology in oil reservoirs undergoing secondary recovery. CONCLUSION: Our data expand current knowledge of the metabolic potential and role in hydrocarbon metabolism of individual members of thermophilic microbial communities from an oil reservoir. The study also reveals potential metabolic exchanges based on vitamin and amino acid auxotrophies indicating the presence of complex network of interactions between microbial taxa within the community. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40168-017-0392-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5756336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57563362018-01-08 Metabolic capability and in situ activity of microorganisms in an oil reservoir Liu, Yi-Fan Galzerani, Daniela Domingos Mbadinga, Serge Maurice Zaramela, Livia S. Gu, Ji-Dong Mu, Bo-Zhong Zengler, Karsten Microbiome Research BACKGROUND: Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microbes are actively participating in crude oil degradation in situ and to gain insight into what metabolic pathways they deploy. RESULTS: Here, we describe the metabolic potential and in situ activity of microbial communities obtained from the Jiangsu Oil Reservoir (China) by an integrated metagenomics and metatranscriptomics approach. Almost complete genome sequences obtained by differential binning highlight the distinct capability of different community members to degrade hydrocarbons under oxic or anoxic condition. Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. Furthermore, nutritional requirements based on amino acid and vitamin auxotrophies suggest a complex network of interactions and dependencies among active community members that go beyond classical syntrophic exchanges; this network defines community composition and microbial ecology in oil reservoirs undergoing secondary recovery. CONCLUSION: Our data expand current knowledge of the metabolic potential and role in hydrocarbon metabolism of individual members of thermophilic microbial communities from an oil reservoir. The study also reveals potential metabolic exchanges based on vitamin and amino acid auxotrophies indicating the presence of complex network of interactions between microbial taxa within the community. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40168-017-0392-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-05 /pmc/articles/PMC5756336/ /pubmed/29304850 http://dx.doi.org/10.1186/s40168-017-0392-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Liu, Yi-Fan Galzerani, Daniela Domingos Mbadinga, Serge Maurice Zaramela, Livia S. Gu, Ji-Dong Mu, Bo-Zhong Zengler, Karsten Metabolic capability and in situ activity of microorganisms in an oil reservoir |
title | Metabolic capability and in situ activity of microorganisms in an oil reservoir |
title_full | Metabolic capability and in situ activity of microorganisms in an oil reservoir |
title_fullStr | Metabolic capability and in situ activity of microorganisms in an oil reservoir |
title_full_unstemmed | Metabolic capability and in situ activity of microorganisms in an oil reservoir |
title_short | Metabolic capability and in situ activity of microorganisms in an oil reservoir |
title_sort | metabolic capability and in situ activity of microorganisms in an oil reservoir |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756336/ https://www.ncbi.nlm.nih.gov/pubmed/29304850 http://dx.doi.org/10.1186/s40168-017-0392-1 |
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