Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets

The DNA coding sequence of TaqStoffel polymerase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. The recombinant enzyme was purified and its enzymatic features were studied. The fusion protein (PfuDBDlig-T...

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Detalles Bibliográficos
Autores principales: Śpibida, Marta, Krawczyk, Beata, Zalewska-Piątek, Beata, Piątek, Rafał, Wysocka, Magdalena, Olszewski, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Biotechnologically Relevant Enzymes and Proteins
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756566/
https://www.ncbi.nlm.nih.gov/pubmed/29103168
http://dx.doi.org/10.1007/s00253-017-8560-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756566/
https://www.ncbi.nlm.nih.gov/pubmed/29103168
http://dx.doi.org/10.1007/s00253-017-8560-6

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